Swept-sine noise-induced damage as a hearing loss model for preclinical assays

Mouse models are key tools for studying cochlear alterations in noise-induced hearing loss (NIHL) and for evaluating new therapies. Stimuli used to induce deafness in mice are usually white and octave band noises that include very low frequencies, considering the large mouse auditory range. We desig...

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Autores: Sanz, Lorena, Murillo-Cuesta, Silvia, Cobo, Pedro, Cediel-Algovia, Rafael, Contreras, Julio, Rivera, Teresa, Varela-Nieto, Isabel, Avendaño Trueba, Carlos
Formato: artículo
Fecha de publicación:2015
País:España
Recursos:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/672359
Acesso em linha:http://hdl.handle.net/10486/672359
https://dx.doi.org/10.3389/fnagi.2015.00007
Access Level:acceso abierto
Palavra-chave:Cytocochleogram
Hair cells
Hearing loss
Transtympanic
TGF-b inhibition
Violet noise
Medicina
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spelling Swept-sine noise-induced damage as a hearing loss model for preclinical assaysSanz, LorenaMurillo-Cuesta, SilviaCobo, PedroCediel-Algovia, RafaelContreras, JulioRivera, TeresaVarela-Nieto, IsabelAvendaño Trueba, CarlosCytocochleogramHair cellsHearing lossTranstympanicTGF-b inhibitionViolet noiseMedicinaMouse models are key tools for studying cochlear alterations in noise-induced hearing loss (NIHL) and for evaluating new therapies. Stimuli used to induce deafness in mice are usually white and octave band noises that include very low frequencies, considering the large mouse auditory range. We designed different sound stimuli, enriched in frequencies up to 20 kHz (“violet” noises) to examine their impact on hearing thresholds and cochlear cytoarchitecture after short exposure. In addition, we developed a cytocochleogram to quantitatively assess the ensuing structural degeneration and its functional correlation. Finally, we used this mouse model and cochleogram procedure to evaluate the potential therapeutic effect of transforming growth factor b1 (TGF-b1) inhibitors P17 and P144 on NIHL. CBA mice were exposed to violet swept-sine noise (VS) with different frequency ranges (2–20 or 9–13 kHz) and levels (105 or 120 dB SPL) for 30 min. Mice were evaluated by auditory brainstem response (ABR) and otoacoustic emission tests prior to and 2, 14 and 28 days after noise exposure. Cochlear pathology was assessed with gross histology; hair cell number was estimated by a stereological counting method. Our results indicate that functional and morphological changes induced by VS depend on the sound level and frequency composition. Partial hearing recovery followed the exposure to 105 dB SPL, whereas permanent cochlear damage resulted from the exposure to 120 dB SPL. Exposure to 9–13 kHz noise caused an auditory threshold shift (TS) in those frequencies that correlated with hair cell loss in the corresponding areas of the cochlea that were spotted on the cytocochleogram. In summary, we present mouse models of NIHL, which depending on the sound properties of the noise, cause different degrees of cochlear damage, and could therefore be used to study molecules which are potential players in hearing loss protection and repairThis work was supported by grants from MINECO (SAF2011- 24391), Fundación de Investigación Médica Mutua Madrileña (FMM2012), European FP7-INNOVA2-AFHELO and FP7- PEOPLE-IAPP-TARGEAR to IVN and FIS PI10/00394 for TR. S.M.-C. holds a CIBERER (ISCIII) postdoctoral contract.Frontiers MediaDepartamento de Anatomía, Histología y NeurocienciaFacultad de MedicinaInstituto de Investigaciones Biomédicas "Alberto Sols" (IIBM)20152015-01-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/672359https://dx.doi.org/10.3389/fnagi.2015.00007reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6723592026-06-23T12:46:27Z
dc.title.none.fl_str_mv Swept-sine noise-induced damage as a hearing loss model for preclinical assays
title Swept-sine noise-induced damage as a hearing loss model for preclinical assays
spellingShingle Swept-sine noise-induced damage as a hearing loss model for preclinical assays
Sanz, Lorena
Cytocochleogram
Hair cells
Hearing loss
Transtympanic
TGF-b inhibition
Violet noise
Medicina
title_short Swept-sine noise-induced damage as a hearing loss model for preclinical assays
title_full Swept-sine noise-induced damage as a hearing loss model for preclinical assays
title_fullStr Swept-sine noise-induced damage as a hearing loss model for preclinical assays
title_full_unstemmed Swept-sine noise-induced damage as a hearing loss model for preclinical assays
title_sort Swept-sine noise-induced damage as a hearing loss model for preclinical assays
dc.creator.none.fl_str_mv Sanz, Lorena
Murillo-Cuesta, Silvia
Cobo, Pedro
Cediel-Algovia, Rafael
Contreras, Julio
Rivera, Teresa
Varela-Nieto, Isabel
Avendaño Trueba, Carlos
author Sanz, Lorena
author_facet Sanz, Lorena
Murillo-Cuesta, Silvia
Cobo, Pedro
Cediel-Algovia, Rafael
Contreras, Julio
Rivera, Teresa
Varela-Nieto, Isabel
Avendaño Trueba, Carlos
author_role author
author2 Murillo-Cuesta, Silvia
Cobo, Pedro
Cediel-Algovia, Rafael
Contreras, Julio
Rivera, Teresa
Varela-Nieto, Isabel
Avendaño Trueba, Carlos
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Anatomía, Histología y Neurociencia
Facultad de Medicina
Instituto de Investigaciones Biomédicas "Alberto Sols" (IIBM)
dc.subject.none.fl_str_mv Cytocochleogram
Hair cells
Hearing loss
Transtympanic
TGF-b inhibition
Violet noise
Medicina
topic Cytocochleogram
Hair cells
Hearing loss
Transtympanic
TGF-b inhibition
Violet noise
Medicina
description Mouse models are key tools for studying cochlear alterations in noise-induced hearing loss (NIHL) and for evaluating new therapies. Stimuli used to induce deafness in mice are usually white and octave band noises that include very low frequencies, considering the large mouse auditory range. We designed different sound stimuli, enriched in frequencies up to 20 kHz (“violet” noises) to examine their impact on hearing thresholds and cochlear cytoarchitecture after short exposure. In addition, we developed a cytocochleogram to quantitatively assess the ensuing structural degeneration and its functional correlation. Finally, we used this mouse model and cochleogram procedure to evaluate the potential therapeutic effect of transforming growth factor b1 (TGF-b1) inhibitors P17 and P144 on NIHL. CBA mice were exposed to violet swept-sine noise (VS) with different frequency ranges (2–20 or 9–13 kHz) and levels (105 or 120 dB SPL) for 30 min. Mice were evaluated by auditory brainstem response (ABR) and otoacoustic emission tests prior to and 2, 14 and 28 days after noise exposure. Cochlear pathology was assessed with gross histology; hair cell number was estimated by a stereological counting method. Our results indicate that functional and morphological changes induced by VS depend on the sound level and frequency composition. Partial hearing recovery followed the exposure to 105 dB SPL, whereas permanent cochlear damage resulted from the exposure to 120 dB SPL. Exposure to 9–13 kHz noise caused an auditory threshold shift (TS) in those frequencies that correlated with hair cell loss in the corresponding areas of the cochlea that were spotted on the cytocochleogram. In summary, we present mouse models of NIHL, which depending on the sound properties of the noise, cause different degrees of cochlear damage, and could therefore be used to study molecules which are potential players in hearing loss protection and repair
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/672359
https://dx.doi.org/10.3389/fnagi.2015.00007
url http://hdl.handle.net/10486/672359
https://dx.doi.org/10.3389/fnagi.2015.00007
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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