TXRF spectrometry in the bioanalytical sciences: A brief review
This work describes the basic principles of Total-reflection X-Ray Fluorescence (TXRF) spectrometry and some of its most outstanding applications for investigating metal traces, mainly in biological and biomedical samples. TXRF is an analytical technique that combines the versatility of elemental an...
| Autor: | |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universidad Autónoma de Madrid |
| Repositorio: | Biblos-e Archivo. Repositorio Institucional de la UAM |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.uam.es:10486/699939 |
| Acceso en línea: | http://hdl.handle.net/10486/699939 https://dx.doi.org/10.1002/xrs.3243 |
| Access Level: | acceso abierto |
| Palabra clave: | X-Ray fluorescence (TXRF) X-ray spectrometric technique Biología y Biomedicina / Biología |
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TXRF spectrometry in the bioanalytical sciences: A brief reviewFernández-Ruiz, RamónX-Ray fluorescence (TXRF)X-ray spectrometric techniqueBiología y Biomedicina / BiologíaThis work describes the basic principles of Total-reflection X-Ray Fluorescence (TXRF) spectrometry and some of its most outstanding applications for investigating metal traces, mainly in biological and biomedical samples. TXRF is an analytical technique that combines the versatility of elemental analysis by X-ray energy dispersion with a low detection limit in the order of picograms of absolute mass or a few ppb (μg L−1) as concentration. The microanalytical capacity of TXRF allows the study of small sample quantities. The TXRF can perform qualitative and mass ratio analyses of the elements present in a sample of only a few hundred nanograms. These properties are of great importance in the research of metal traces in biological systems, as they allow fast, precise, and accurate characterisation of the elemental fingerprint at trace level. Its successful application in studies of the coordination kinetics of new platinum-based antitumor drugs with DNA, its application in the study profile of the metal in healthy and cancerous human tissues, or even its application in the study of the processes of metal diffusion through cell membranes are just a few examples of TXRF capabilities in the biomedical sciences. This brief review's main objective is to provide an overview of the physical principles and possibilities of TXRF spectrometry. It also highlights some of the most outstanding applications that have been developed so far, mainly in the biological field, so that the reader can evaluate their potential applicationsThis article is into the frame of the COST Action CA18130 ENFORCE TXRF, supported by COST (European Cooperation in Science and Technology)WileyOtras Unidades de la UAM20212021-06-02research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/699939https://dx.doi.org/10.1002/xrs.3243reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6999392026-06-23T12:46:27Z |
| dc.title.none.fl_str_mv |
TXRF spectrometry in the bioanalytical sciences: A brief review |
| title |
TXRF spectrometry in the bioanalytical sciences: A brief review |
| spellingShingle |
TXRF spectrometry in the bioanalytical sciences: A brief review Fernández-Ruiz, Ramón X-Ray fluorescence (TXRF) X-ray spectrometric technique Biología y Biomedicina / Biología |
| title_short |
TXRF spectrometry in the bioanalytical sciences: A brief review |
| title_full |
TXRF spectrometry in the bioanalytical sciences: A brief review |
| title_fullStr |
TXRF spectrometry in the bioanalytical sciences: A brief review |
| title_full_unstemmed |
TXRF spectrometry in the bioanalytical sciences: A brief review |
| title_sort |
TXRF spectrometry in the bioanalytical sciences: A brief review |
| dc.creator.none.fl_str_mv |
Fernández-Ruiz, Ramón |
| author |
Fernández-Ruiz, Ramón |
| author_facet |
Fernández-Ruiz, Ramón |
| author_role |
author |
| dc.contributor.none.fl_str_mv |
Otras Unidades de la UAM |
| dc.subject.none.fl_str_mv |
X-Ray fluorescence (TXRF) X-ray spectrometric technique Biología y Biomedicina / Biología |
| topic |
X-Ray fluorescence (TXRF) X-ray spectrometric technique Biología y Biomedicina / Biología |
| description |
This work describes the basic principles of Total-reflection X-Ray Fluorescence (TXRF) spectrometry and some of its most outstanding applications for investigating metal traces, mainly in biological and biomedical samples. TXRF is an analytical technique that combines the versatility of elemental analysis by X-ray energy dispersion with a low detection limit in the order of picograms of absolute mass or a few ppb (μg L−1) as concentration. The microanalytical capacity of TXRF allows the study of small sample quantities. The TXRF can perform qualitative and mass ratio analyses of the elements present in a sample of only a few hundred nanograms. These properties are of great importance in the research of metal traces in biological systems, as they allow fast, precise, and accurate characterisation of the elemental fingerprint at trace level. Its successful application in studies of the coordination kinetics of new platinum-based antitumor drugs with DNA, its application in the study profile of the metal in healthy and cancerous human tissues, or even its application in the study of the processes of metal diffusion through cell membranes are just a few examples of TXRF capabilities in the biomedical sciences. This brief review's main objective is to provide an overview of the physical principles and possibilities of TXRF spectrometry. It also highlights some of the most outstanding applications that have been developed so far, mainly in the biological field, so that the reader can evaluate their potential applications |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2021-06-02 |
| dc.type.none.fl_str_mv |
research article http://purl.org/coar/resource_type/c_2df8fbb1 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
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article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10486/699939 https://dx.doi.org/10.1002/xrs.3243 |
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http://hdl.handle.net/10486/699939 https://dx.doi.org/10.1002/xrs.3243 |
| dc.language.none.fl_str_mv |
Inglés eng |
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Inglés |
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eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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Wiley |
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Wiley |
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reponame:Biblos-e Archivo. Repositorio Institucional de la UAM instname:Universidad Autónoma de Madrid |
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Universidad Autónoma de Madrid |
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Biblos-e Archivo. Repositorio Institucional de la UAM |
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Biblos-e Archivo. Repositorio Institucional de la UAM |
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