Fasting, crowding and temperature effects on the cardiac activity of European seabass during ice-slurry slaughtering procedure

Aquaculture production continues to expand rapidly, highlighting the need to improve welfare standards during harvesting procedures. Pre-slaughter operations such as fasting and crowding, along with slaughter procedures, represent critical points with significant welfare implications. In this study,...

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Autores: Hoyo-Albarez, E., Cabrera-Álvarez, M.J., Arechavala-Lopez, P., Roque, Ana
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/4723
Acceso en línea:http://hdl.handle.net/20.500.12327/4723
https://doi.org/10.1016/j.aqrep.2025.102972
Access Level:acceso abierto
Palabra clave:637
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spelling Fasting, crowding and temperature effects on the cardiac activity of European seabass during ice-slurry slaughtering procedureHoyo-Albarez, E.Cabrera-Álvarez, M.J.Arechavala-Lopez, P.Roque, Ana637Aquaculture production continues to expand rapidly, highlighting the need to improve welfare standards during harvesting procedures. Pre-slaughter operations such as fasting and crowding, along with slaughter procedures, represent critical points with significant welfare implications. In this study, the effects of pre-slaughter protocols and seasonal temperature on cardiac responses during slaughter in European seabass (Dicentrarchus labrax) were evaluated. To monitor cardiac activity, biologgers were surgically implanted in pre-adult seabass subjected to fasting periods and crowding protocols, followed by slaughter in ice slurry or ikejime (a rapid killing method involving brain destruction) at different seasonal temperatures (summer and winter). Results revealed that fasting significantly reduced the time to loss of cardiac function during ice slurry slaughter, particularly under warmer conditions, as fasted individuals lost cardiac activity 14 min earlier than controls, likely due to energy depletion. Crowding elevated heart rate during ice slurry slaughter, counteracting the expected bradycardic response to ice, particularly in summer, where crowded fish lost cardiac signal 11 min earlier than controls. Seasonal variation in water temperature strongly influenced physiological outcomes: winter trials resulted in markedly prolonged cardiac activity during ice exposure, with cessation of cardiac activity times up to 219 min in fasted fish, compared to 32 min in summer. Additionally, crowded fish in summer showed sustained high heart rates and faster cardiac cesssation, indicating acute stress despite shortened duration. These findings suggest that pre-slaughter stressors and environmental conditions interact to shape welfare outcomes. While fasting may improve slaughter efficiency during summer, crowding under high temperatures poses a welfare concern. From a welfare-oriented perspective, minimising crowding and tailoring fasting protocols to seasonal conditions are recommended to improve slaughter efficiency and reduce stress in seabass aquaculture.This study is part of the WELLSTUN project: "Improvement of the slaughter process in farmed fish: Welfare indicators and product quality", funded by the National Aquaculture Plan of the Ministry of Agriculture, Fisheries, and Food from the Spanish Government coordinated by AR. Additionally, EH-A was supported by a “SOIB-Recerca i Innovació” contract, P. A-L was supported by a Ramón y Cajal contract (RYC2020–029629-I) from the State Research Agency of the Spanish Government, and M.J.C-A received Portuguese national funds from FCT - Foundation for Science and Technology through projects UIDB/04326/2020 (DOI:10.54499/UIDB/04326/2020) and LA/P/0101/2020 (DOI:10.54499/LA/P/0101/2020). The authors acknowledge the staff of IRTA for their support and assistance during the experiments.info:eu-repo/semantics/publishedVersionElsevierProducció AnimalAqüicultura2025info:eu-repo/semantics/article8http://hdl.handle.net/20.500.12327/4723https://doi.org/10.1016/j.aqrep.2025.102972reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésAquaculture ReportsMAPA/ / /ES/Mejora del proceso de sacrificio de los peces de cultivo: indicadores de bienestar y calidad del producto/WELLSTUNMICINN/ /RYC2020-029629-I/ES/ /Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/47232026-05-29T05:05:01Z
dc.title.none.fl_str_mv Fasting, crowding and temperature effects on the cardiac activity of European seabass during ice-slurry slaughtering procedure
title Fasting, crowding and temperature effects on the cardiac activity of European seabass during ice-slurry slaughtering procedure
spellingShingle Fasting, crowding and temperature effects on the cardiac activity of European seabass during ice-slurry slaughtering procedure
Hoyo-Albarez, E.
637
title_short Fasting, crowding and temperature effects on the cardiac activity of European seabass during ice-slurry slaughtering procedure
title_full Fasting, crowding and temperature effects on the cardiac activity of European seabass during ice-slurry slaughtering procedure
title_fullStr Fasting, crowding and temperature effects on the cardiac activity of European seabass during ice-slurry slaughtering procedure
title_full_unstemmed Fasting, crowding and temperature effects on the cardiac activity of European seabass during ice-slurry slaughtering procedure
title_sort Fasting, crowding and temperature effects on the cardiac activity of European seabass during ice-slurry slaughtering procedure
dc.creator.none.fl_str_mv Hoyo-Albarez, E.
Cabrera-Álvarez, M.J.
Arechavala-Lopez, P.
Roque, Ana
author Hoyo-Albarez, E.
author_facet Hoyo-Albarez, E.
Cabrera-Álvarez, M.J.
Arechavala-Lopez, P.
Roque, Ana
author_role author
author2 Cabrera-Álvarez, M.J.
Arechavala-Lopez, P.
Roque, Ana
author2_role author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Aqüicultura
dc.subject.none.fl_str_mv 637
topic 637
description Aquaculture production continues to expand rapidly, highlighting the need to improve welfare standards during harvesting procedures. Pre-slaughter operations such as fasting and crowding, along with slaughter procedures, represent critical points with significant welfare implications. In this study, the effects of pre-slaughter protocols and seasonal temperature on cardiac responses during slaughter in European seabass (Dicentrarchus labrax) were evaluated. To monitor cardiac activity, biologgers were surgically implanted in pre-adult seabass subjected to fasting periods and crowding protocols, followed by slaughter in ice slurry or ikejime (a rapid killing method involving brain destruction) at different seasonal temperatures (summer and winter). Results revealed that fasting significantly reduced the time to loss of cardiac function during ice slurry slaughter, particularly under warmer conditions, as fasted individuals lost cardiac activity 14 min earlier than controls, likely due to energy depletion. Crowding elevated heart rate during ice slurry slaughter, counteracting the expected bradycardic response to ice, particularly in summer, where crowded fish lost cardiac signal 11 min earlier than controls. Seasonal variation in water temperature strongly influenced physiological outcomes: winter trials resulted in markedly prolonged cardiac activity during ice exposure, with cessation of cardiac activity times up to 219 min in fasted fish, compared to 32 min in summer. Additionally, crowded fish in summer showed sustained high heart rates and faster cardiac cesssation, indicating acute stress despite shortened duration. These findings suggest that pre-slaughter stressors and environmental conditions interact to shape welfare outcomes. While fasting may improve slaughter efficiency during summer, crowding under high temperatures poses a welfare concern. From a welfare-oriented perspective, minimising crowding and tailoring fasting protocols to seasonal conditions are recommended to improve slaughter efficiency and reduce stress in seabass aquaculture.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/4723
https://doi.org/10.1016/j.aqrep.2025.102972
url http://hdl.handle.net/20.500.12327/4723
https://doi.org/10.1016/j.aqrep.2025.102972
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Aquaculture Reports
MAPA/ / /ES/Mejora del proceso de sacrificio de los peces de cultivo: indicadores de bienestar y calidad del producto/WELLSTUN
MICINN/ /RYC2020-029629-I/ES/ /
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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