Crystal organisation and material properties of Chama and Glycymeris myostraca and shells

Movement of bivalve hard and soft tissue requires muscular action. Despite diverse bivalve lifestyles and living environments, the myostracum, a specific hard tissue formed where muscles attach to the shell, appears similar in structure for species of many bivalve orders. We investigated myostracal...

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Detalles Bibliográficos
Autores: Hoerl, S., le Moine, T., Peter, N. J., Amini, Sajad Ghaed, Griesshaber, Erika, Wang, J., Salas, C., Checa, Antonio G., Schwaiger, R., Schmahl, Wolfgang W.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/372297
Acceso en línea:http://hdl.handle.net/10261/372297
Access Level:acceso abierto
Palabra clave:Backscattering
Hardness
Molluscs
Nanoindentation
Textures
Twinning
Bivalve
Crystal material
Crystal organization
EBSD
Hard tissues
Microstructure-texture
Muscular actions
Myostracum/muscle scar
Nano indentation
Soft tissue
Descripción
Sumario:Movement of bivalve hard and soft tissue requires muscular action. Despite diverse bivalve lifestyles and living environments, the myostracum, a specific hard tissue formed where muscles attach to the shell, appears similar in structure for species of many bivalve orders. We investigated myostracal and non-myostracal, valve, microstructure, texture and material properties of Chamidae and Glycymerididae species with electron-backscatter-diffraction, laser-confocal and backscatter electron imaging and nanoindentation testing. Chamidae and Glycymerididae follow different lifestyles and live in distinct environments. Chamidae are cemented to substrate and live in wave-swept, shallow, waters. Glycymerididae dwell in calm water and burrow into sandy/muddy sediment. We found that myostracal aragonite of all investigated species has a crystal assembly pattern that reflects crystal growth through growth competition. Aragonite is extensively twinned in the myostracum and non-myostracal, valve, layers, not in the calcitic ornamentation. For myostracal aragonite, we found cyclic twinning, for non-myostracal aragonite the twinning was polysynthetic or polycyclic. We show how twinning and crystallographic texture are transmitted between myostracal and non-myostracal, valve, layers. Relative to non-biological aragonite, myostracal and non-myostracal, valve, indentation elastic modulus is reduced by 10–15 % and 15–20 %, respectively; myostracal and valve hardness is increased by 15–20 % and 5–10 %, respectively. Comparing modulus and hardness between aragonitic microstructures, we found that, relative to other microstructures, myostracal modulus is increased by 5 % and myostracal hardness by 15 %. Hence, the myostracal material shows a unique and specific microstructure, texture, modulus, and hardness that might be necessary for muscle attachment to enable the lifestyle-controlled requirements posed onto the organism.