Snake venom disintegrins: novel dimeric disintegrins and structural diversification by disulphide bond engineering

The final version of record is available at: http://www.biochemj.org/bj/default.htm

Detalles Bibliográficos
Autores: Calvete, Juan J., Moreno-Murciano, Paz, Theakston, R David G, Kisiel, Dariusz G, Marcinkiewicz, Cezary
Tipo de recurso: artículo
Fecha de publicación:2003
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/3631
Acceso en línea:http://dx.doi.org/10.1042/BJ20021739
http://hdl.handle.net/10261/3631
Access Level:acceso abierto
Palabra clave:Disintegrin
Disulphide bond
Evolution
Protein sequence
Snake venom protein
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spelling Snake venom disintegrins: novel dimeric disintegrins and structural diversification by disulphide bond engineeringCalvete, Juan J.Moreno-Murciano, PazTheakston, R David GKisiel, Dariusz GMarcinkiewicz, CezaryDisintegrinDisulphide bondEvolutionProtein sequenceSnake venom proteinThe final version of record is available at: http://www.biochemj.org/bj/default.htmWe report the isolation and amino acid sequences of six novel dimeric disintegrins from the venoms of Vipera lebetina obtusa (VLO), V. berus (VB), V. ammodytes (VA), Echis ocellatus (EO) and Echis multisquamatus (EMS). Disintegrins VLO4, VB7, VA6 and EO4 displayed the RGD motif and inhibited the adhesion of K562 cells, expressing the integrin alpha5beta1 to immobilized fibronectin. A second group of dimeric disintegrins (VLO5 and EO5) had MLD and VGD motifs in their subunits and blocked the adhesion of the alpha4beta1 integrin to vascular cell adhesion molecule 1 with high selectivity. On the other hand, disintegrin EMS11 inhibited both alpha5beta1 and alpha4beta1 integrins with almost the same degree of specificity. Comparison of the amino acid sequences of the dimeric disintegrins with those of other disintegrins by multiple-sequence alignment and phylogenetic analysis, in conjunction with current biochemical and genetic data, supports the view that the different disintegrin subfamilies evolved from a common ADAM (a disintegrin and metalloproteinase-like) scaffold and that structural diversification occurred through disulphide bond engineering.This work was financed by the Dirección General de Enseñanza Superior e Investigación Científica (Madrid, Spain) (grant no. BMC2001-3337; to J. J. C.) and the American Heart Association (Dallas, TX, U.S.A.; to C. M.).Peer reviewedBiochemical Society200820082003info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501510722 bytesapplication/pdfhttp://dx.doi.org/10.1042/BJ20021739http://hdl.handle.net/10261/3631reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/36312026-05-22T06:33:51Z
dc.title.none.fl_str_mv Snake venom disintegrins: novel dimeric disintegrins and structural diversification by disulphide bond engineering
title Snake venom disintegrins: novel dimeric disintegrins and structural diversification by disulphide bond engineering
spellingShingle Snake venom disintegrins: novel dimeric disintegrins and structural diversification by disulphide bond engineering
Calvete, Juan J.
Disintegrin
Disulphide bond
Evolution
Protein sequence
Snake venom protein
title_short Snake venom disintegrins: novel dimeric disintegrins and structural diversification by disulphide bond engineering
title_full Snake venom disintegrins: novel dimeric disintegrins and structural diversification by disulphide bond engineering
title_fullStr Snake venom disintegrins: novel dimeric disintegrins and structural diversification by disulphide bond engineering
title_full_unstemmed Snake venom disintegrins: novel dimeric disintegrins and structural diversification by disulphide bond engineering
title_sort Snake venom disintegrins: novel dimeric disintegrins and structural diversification by disulphide bond engineering
dc.creator.none.fl_str_mv Calvete, Juan J.
Moreno-Murciano, Paz
Theakston, R David G
Kisiel, Dariusz G
Marcinkiewicz, Cezary
author Calvete, Juan J.
author_facet Calvete, Juan J.
Moreno-Murciano, Paz
Theakston, R David G
Kisiel, Dariusz G
Marcinkiewicz, Cezary
author_role author
author2 Moreno-Murciano, Paz
Theakston, R David G
Kisiel, Dariusz G
Marcinkiewicz, Cezary
author2_role author
author
author
author
dc.subject.none.fl_str_mv Disintegrin
Disulphide bond
Evolution
Protein sequence
Snake venom protein
topic Disintegrin
Disulphide bond
Evolution
Protein sequence
Snake venom protein
description The final version of record is available at: http://www.biochemj.org/bj/default.htm
publishDate 2003
dc.date.none.fl_str_mv 2003
2008
2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://dx.doi.org/10.1042/BJ20021739
http://hdl.handle.net/10261/3631
url http://dx.doi.org/10.1042/BJ20021739
http://hdl.handle.net/10261/3631
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 510722 bytes
application/pdf
dc.publisher.none.fl_str_mv Biochemical Society
publisher.none.fl_str_mv Biochemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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