The CYP71A, NIT, AMI, and IAMH gene families are dispensable for indole-3-acetaldoxime-mediated auxin biosynthesis in Arabidopsis

[EN] The auxin indole-3-acetic acid (IAA) governs plant development and environmental responses. Although the indole-3-pyruvic acid (IPyA) pathway is the predominant route for IAA biosynthesis, other pathways have been proposed, such as the indole-3-acetaldoxime (IAOx) pathway. The IAOx pathway has...

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Autores: Fenech, Mario, Brumos, Javier, Pencik, Ales, Edwards, Brianne, Belcapo, Sara, Delacey, Jennifer, Patel, Arjun, Kater, Martin, Li, Xu, Ljung, Karin, Novak, Ondrej, Alonso, Jose M., Stepanova, Anna N.
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/230232
Acceso en línea:https://riunet.upv.es/handle/10251/230232
Access Level:acceso abierto
Palabra clave:Indole-3-acetic acid
Auxin biosynthesis
Indole-3-acetaldoxime pathway
Arabidopsis thaliana
Indole-3-acetonitrile
Indole-3-acetamide
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oai_identifier_str oai:riunet.upv.es:10251/230232
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv The CYP71A, NIT, AMI, and IAMH gene families are dispensable for indole-3-acetaldoxime-mediated auxin biosynthesis in Arabidopsis
title The CYP71A, NIT, AMI, and IAMH gene families are dispensable for indole-3-acetaldoxime-mediated auxin biosynthesis in Arabidopsis
spellingShingle The CYP71A, NIT, AMI, and IAMH gene families are dispensable for indole-3-acetaldoxime-mediated auxin biosynthesis in Arabidopsis
Fenech, Mario
Indole-3-acetic acid
Auxin biosynthesis
Indole-3-acetaldoxime pathway
Arabidopsis thaliana
Indole-3-acetonitrile
Indole-3-acetamide
title_short The CYP71A, NIT, AMI, and IAMH gene families are dispensable for indole-3-acetaldoxime-mediated auxin biosynthesis in Arabidopsis
title_full The CYP71A, NIT, AMI, and IAMH gene families are dispensable for indole-3-acetaldoxime-mediated auxin biosynthesis in Arabidopsis
title_fullStr The CYP71A, NIT, AMI, and IAMH gene families are dispensable for indole-3-acetaldoxime-mediated auxin biosynthesis in Arabidopsis
title_full_unstemmed The CYP71A, NIT, AMI, and IAMH gene families are dispensable for indole-3-acetaldoxime-mediated auxin biosynthesis in Arabidopsis
title_sort The CYP71A, NIT, AMI, and IAMH gene families are dispensable for indole-3-acetaldoxime-mediated auxin biosynthesis in Arabidopsis
dc.creator.none.fl_str_mv Fenech, Mario
Brumos, Javier
Pencik, Ales
Edwards, Brianne
Belcapo, Sara
Delacey, Jennifer
Patel, Arjun
Kater, Martin
Li, Xu
Ljung, Karin
Novak, Ondrej
Alonso, Jose M.
Stepanova, Anna N.
author Fenech, Mario
author_facet Fenech, Mario
Brumos, Javier
Pencik, Ales
Edwards, Brianne
Belcapo, Sara
Delacey, Jennifer
Patel, Arjun
Kater, Martin
Li, Xu
Ljung, Karin
Novak, Ondrej
Alonso, Jose M.
Stepanova, Anna N.
author_role author
author2 Brumos, Javier
Pencik, Ales
Edwards, Brianne
Belcapo, Sara
Delacey, Jennifer
Patel, Arjun
Kater, Martin
Li, Xu
Ljung, Karin
Novak, Ondrej
Alonso, Jose M.
Stepanova, Anna N.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Swedish Research Council
National Science Foundation, EEUU
Knut and Alice Wallenberg Foundation
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Indole-3-acetic acid
Auxin biosynthesis
Indole-3-acetaldoxime pathway
Arabidopsis thaliana
Indole-3-acetonitrile
Indole-3-acetamide
topic Indole-3-acetic acid
Auxin biosynthesis
Indole-3-acetaldoxime pathway
Arabidopsis thaliana
Indole-3-acetonitrile
Indole-3-acetamide
description [EN] The auxin indole-3-acetic acid (IAA) governs plant development and environmental responses. Although the indole-3-pyruvic acid (IPyA) pathway is the predominant route for IAA biosynthesis, other pathways have been proposed, such as the indole-3-acetaldoxime (IAOx) pathway. The IAOx pathway has garnered attention due to its supposed activation in auxin-overproducing mutants (e.g. sur1, sur2, ugt74b1) and the auxin-like responses triggered by exogenous application of its proposed intermediates IAOx, indole-3-acetonitrile (IAN), and indole-3-acetamide (IAM). However, despite the supporting evidence for individual steps of the IAOx pathway, its overall physiological relevance remains inconclusive. Here, using a comprehensive genetic approach combined with metabolic and phenotypic profiling, we demonstrate that mutating gene families proposed to function in the IAOx pathway in Arabidopsis (Arabidopsis thaliana) does not result in prominent auxin-deficient phenotypes, nor are these genes required for the high auxin production in the sur2 mutant. Our findings also challenge the previously postulated linear IAOx pathway. Exogenously provided IAOx, IAN, and IAM can be converted to IAA in vivo, but they do not act as precursors for each other. Finally, our findings question the physiological relevance of IAM and IAN as IAA precursors in plants and suggest the existence of a yet-uncharacterized route for IAA production in the sur2 mutant, likely involving IAOx as an intermediate. The identification of the metabolic steps and the corresponding genes in this pathway may uncover another IAA biosynthesis route in plants.; Research challenges previously postulated genetic pathways for the conversion of indole-3-acetaldoxime into indole-3-acetic acid in the auxin-overproducing sur2 mutant in Arabidopsis.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-11-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/230232
url https://riunet.upv.es/handle/10251/230232
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv National Science Foundation, China https://doi.org/10.13039/100000001 IOS 1444561
National Science Foundation, China https://doi.org/10.13039/100000001 2327912
National Science Foundation, China https://doi.org/10.13039/100000001 1750006
National Science Foundation, China https://doi.org/10.13039/100000001 1624613
National Science Foundation, China https://doi.org/10.13039/100000001 MCB-0923727
National Science Foundation, China https://doi.org/10.13039/100000001 MCB-1158181
National Science Foundation, China https://doi.org/10.13039/100000001 0519869
Knut and Alice Wallenberg Foundation Knut and Alice Wallenberg Foundation 2016.0352
Knut and Alice Wallenberg Foundation Knut and Alice Wallenberg Foundation 2020.0240
Swedish Research Council https://doi.org/10.13039/501100004359 2021-04938
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologists
publisher.none.fl_str_mv American Society of Plant Biologists
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869409852196913152
spelling The CYP71A, NIT, AMI, and IAMH gene families are dispensable for indole-3-acetaldoxime-mediated auxin biosynthesis in ArabidopsisFenech, MarioBrumos, JavierPencik, AlesEdwards, BrianneBelcapo, SaraDelacey, JenniferPatel, ArjunKater, MartinLi, XuLjung, KarinNovak, OndrejAlonso, Jose M.Stepanova, Anna N.Indole-3-acetic acidAuxin biosynthesisIndole-3-acetaldoxime pathwayArabidopsis thalianaIndole-3-acetonitrileIndole-3-acetamide[EN] The auxin indole-3-acetic acid (IAA) governs plant development and environmental responses. Although the indole-3-pyruvic acid (IPyA) pathway is the predominant route for IAA biosynthesis, other pathways have been proposed, such as the indole-3-acetaldoxime (IAOx) pathway. The IAOx pathway has garnered attention due to its supposed activation in auxin-overproducing mutants (e.g. sur1, sur2, ugt74b1) and the auxin-like responses triggered by exogenous application of its proposed intermediates IAOx, indole-3-acetonitrile (IAN), and indole-3-acetamide (IAM). However, despite the supporting evidence for individual steps of the IAOx pathway, its overall physiological relevance remains inconclusive. Here, using a comprehensive genetic approach combined with metabolic and phenotypic profiling, we demonstrate that mutating gene families proposed to function in the IAOx pathway in Arabidopsis (Arabidopsis thaliana) does not result in prominent auxin-deficient phenotypes, nor are these genes required for the high auxin production in the sur2 mutant. Our findings also challenge the previously postulated linear IAOx pathway. Exogenously provided IAOx, IAN, and IAM can be converted to IAA in vivo, but they do not act as precursors for each other. Finally, our findings question the physiological relevance of IAM and IAN as IAA precursors in plants and suggest the existence of a yet-uncharacterized route for IAA production in the sur2 mutant, likely involving IAOx as an intermediate. The identification of the metabolic steps and the corresponding genes in this pathway may uncover another IAA biosynthesis route in plants.; Research challenges previously postulated genetic pathways for the conversion of indole-3-acetaldoxime into indole-3-acetic acid in the auxin-overproducing sur2 mutant in Arabidopsis.This work was supported by the National Science Foundation (NSF) grants 0923727, 1444561, and 2327912 to J.M.A. and A.N.S.; 1158181 and 0519869 to J.M.A.; and 1750006 to A.N.S., and leveraged microscopy equipment available at the NCSU s Cellular and Molecular Imaging Facility (NSF grant 1624613). S.B. was supported by EVOFRULAND H20MC_RISE21LCOLO_02 CUP G45F21002810006. K.L. acknowledges grants from the Knut and Alice Wallenberg Foundation (KAW 2016.0352, KAW 2020.0240) and the Swedish Research Council (VR 2021-04938).American Society of Plant BiologistsSwedish Research CouncilNational Science Foundation, EEUUKnut and Alice Wallenberg FoundationRepositorio Institucional de la Universitat Politècnica de València Riunet20252025-11-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/230232reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengNational Science Foundation, China https://doi.org/10.13039/100000001 IOS 1444561National Science Foundation, China https://doi.org/10.13039/100000001 2327912National Science Foundation, China https://doi.org/10.13039/100000001 1750006National Science Foundation, China https://doi.org/10.13039/100000001 1624613National Science Foundation, China https://doi.org/10.13039/100000001 MCB-0923727National Science Foundation, China https://doi.org/10.13039/100000001 MCB-1158181National Science Foundation, China https://doi.org/10.13039/100000001 0519869Knut and Alice Wallenberg Foundation Knut and Alice Wallenberg Foundation 2016.0352Knut and Alice Wallenberg Foundation Knut and Alice Wallenberg Foundation 2020.0240Swedish Research Council https://doi.org/10.13039/501100004359 2021-04938open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2302322026-06-13T07:49:27Z
score 15,811543

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