Survey of Conductive Polymers for the Fabrication of Conformation Switching Nucleic Acid-Based Electrochemical Biosensors

Electrochemical biosensors are a continuously evolving technology with great potential for applications in human health. With the continuous glucose monitor as an example, these sensors are capable of accurately determining molecular concentrations directly in the human body. A specific class of bio...

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Detalhes bibliográficos
Autores: Shaver, Alexander, Mallires, Kyle, Harris, Jonathan, Kavner, Jonathan, Wang, Bo, Gottlieb, Rebecca, Lión Villar, Juan, Herranz Astudillo, María Ángeles, Martín León, Nazario, Arroyo-Currás, Netzahualcóyotl
Formato: artículo
Fecha de publicación:2023
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/120031
Acesso em linha:https://hdl.handle.net/20.500.14352/120031
Access Level:acceso abierto
Palavra-chave:54
Electrochemical sensor
Nucleic acids
Redox reporter
Thiophene
Ciencias
23 Química
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spelling Survey of Conductive Polymers for the Fabrication of Conformation Switching Nucleic Acid-Based Electrochemical BiosensorsShaver, AlexanderMallires, KyleHarris, JonathanKavner, JonathanWang, BoGottlieb, RebeccaLión Villar, JuanHerranz Astudillo, María ÁngelesMartín León, NazarioArroyo-Currás, Netzahualcóyotl54Electrochemical sensorNucleic acidsRedox reporterThiopheneThiopheneCiencias23 QuímicaElectrochemical biosensors are a continuously evolving technology with great potential for applications in human health. With the continuous glucose monitor as an example, these sensors are capable of accurately determining molecular concentrations directly in the human body. A specific class of biosensors, termed conformation switching nucleic acid-based electrochemical sensors (NBEs), relies on the affinity of oligonucleotides for molecular recognition and their conformational dynamics upon target binding for signal generation. Currently, most NBEs are fabricated via the self-assembly of alkylthiol monolayers on Au electrodes. However, this architecture is limited in terms of stability and the breadth of supporting materials with which it is compatible. Here, to explore alternative material options for the fabrication of NBE sensors, we form conductive polymers of aromatic amines, thiophenes, and pyrroles on platinum electrodes. Altering many parameters throughout the study, we determine the extent to which the polymers passivate the electrode surface and then couple redox reporters or reporter-modified nucleic acids. We discuss the limitations and benefits of each polymer for the formation of NBE sensors and provide future directions to continue the quest for alternative sensor materials.American Chemical SocietyUniversidad Complutense de Madrid20232023-12-2820232023-12-28journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://hdl.handle.net/20.500.14352/120031reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1200312026-06-02T12:44:21Z
dc.title.none.fl_str_mv Survey of Conductive Polymers for the Fabrication of Conformation Switching Nucleic Acid-Based Electrochemical Biosensors
title Survey of Conductive Polymers for the Fabrication of Conformation Switching Nucleic Acid-Based Electrochemical Biosensors
spellingShingle Survey of Conductive Polymers for the Fabrication of Conformation Switching Nucleic Acid-Based Electrochemical Biosensors
Shaver, Alexander
54
Electrochemical sensor
Nucleic acids
Redox reporter
Thiophene
Thiophene
Ciencias
23 Química
title_short Survey of Conductive Polymers for the Fabrication of Conformation Switching Nucleic Acid-Based Electrochemical Biosensors
title_full Survey of Conductive Polymers for the Fabrication of Conformation Switching Nucleic Acid-Based Electrochemical Biosensors
title_fullStr Survey of Conductive Polymers for the Fabrication of Conformation Switching Nucleic Acid-Based Electrochemical Biosensors
title_full_unstemmed Survey of Conductive Polymers for the Fabrication of Conformation Switching Nucleic Acid-Based Electrochemical Biosensors
title_sort Survey of Conductive Polymers for the Fabrication of Conformation Switching Nucleic Acid-Based Electrochemical Biosensors
dc.creator.none.fl_str_mv Shaver, Alexander
Mallires, Kyle
Harris, Jonathan
Kavner, Jonathan
Wang, Bo
Gottlieb, Rebecca
Lión Villar, Juan
Herranz Astudillo, María Ángeles
Martín León, Nazario
Arroyo-Currás, Netzahualcóyotl
author Shaver, Alexander
author_facet Shaver, Alexander
Mallires, Kyle
Harris, Jonathan
Kavner, Jonathan
Wang, Bo
Gottlieb, Rebecca
Lión Villar, Juan
Herranz Astudillo, María Ángeles
Martín León, Nazario
Arroyo-Currás, Netzahualcóyotl
author_role author
author2 Mallires, Kyle
Harris, Jonathan
Kavner, Jonathan
Wang, Bo
Gottlieb, Rebecca
Lión Villar, Juan
Herranz Astudillo, María Ángeles
Martín León, Nazario
Arroyo-Currás, Netzahualcóyotl
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 54
Electrochemical sensor
Nucleic acids
Redox reporter
Thiophene
Thiophene
Ciencias
23 Química
topic 54
Electrochemical sensor
Nucleic acids
Redox reporter
Thiophene
Thiophene
Ciencias
23 Química
description Electrochemical biosensors are a continuously evolving technology with great potential for applications in human health. With the continuous glucose monitor as an example, these sensors are capable of accurately determining molecular concentrations directly in the human body. A specific class of biosensors, termed conformation switching nucleic acid-based electrochemical sensors (NBEs), relies on the affinity of oligonucleotides for molecular recognition and their conformational dynamics upon target binding for signal generation. Currently, most NBEs are fabricated via the self-assembly of alkylthiol monolayers on Au electrodes. However, this architecture is limited in terms of stability and the breadth of supporting materials with which it is compatible. Here, to explore alternative material options for the fabrication of NBE sensors, we form conductive polymers of aromatic amines, thiophenes, and pyrroles on platinum electrodes. Altering many parameters throughout the study, we determine the extent to which the polymers passivate the electrode surface and then couple redox reporters or reporter-modified nucleic acids. We discuss the limitations and benefits of each polymer for the formation of NBE sensors and provide future directions to continue the quest for alternative sensor materials.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-12-28
2023
2023-12-28
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/120031
url https://hdl.handle.net/20.500.14352/120031
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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