Microfluidics-Based Ionic Catch and Release Oligosaccharide Synthesis (ICROS-Microflow) to Expedite Glycosylation Chemistry

A continuous microfluidic glycosylation strategy that requires no chromatography between steps and significantly expedites glycosylation chemistry is described. This practical approach incorporates the advantages of imidazolium-based chromatography-free purification and in situ mass spectrometry rea...

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Detalles Bibliográficos
Autores: Zhang, YaoYao, Ghirardello, Mattia, Williams, Ryan, Silva-Díaz, Adrián, Rojo, Javier, Voglmeir, Josef, Ramos-Soriano, Javier, Galán, M. Carmen
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/381777
Acceso en línea:http://hdl.handle.net/10261/381777
https://api.elsevier.com/content/abstract/scopus_id/85206972435
Access Level:acceso abierto
Palabra clave:Oligosaccharide synthesis
ITag
ICROS
Glycosylation
Continuous flow
Descripción
Sumario:A continuous microfluidic glycosylation strategy that requires no chromatography between steps and significantly expedites glycosylation chemistry is described. This practical approach incorporates the advantages of imidazolium-based chromatography-free purification and in situ mass spectrometry reaction monitoring to achieve fast reaction optimization and shorter reaction times. We demonstrate the utility of this strategy in the synthesis of a series of glycoside targets, including an α-(1,6)-trimannoside and a branched Man5 oligomannoside, within 1 day.