Estudio cuantitativo de lipofuscina mediante microscopía de fluorescencia en cerebros de crustáceos peneidos: aplicación a la determinación de la edad en animales salvajes Parapenaeus longirostris (Lucas, 1846) y Aristeus antennatus ...
The knowledge on the age structure in exploited crustacean populations is essential not only to undertand their population dynamics but also for an effective stock assessment and management. In the present work, a novel methodology based on the quantification of the agepigment lipofuscin has been us...
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| Tipo de recurso: | tesis doctoral |
| Fecha de publicación: | 2005 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/318972 |
| Acceso en línea: | http://hdl.handle.net/10261/318972 |
| Access Level: | acceso abierto |
| Palabra clave: | Lipofuscina Medio Marino Estimación de la edad Crustaceos Marsupenaeus japonicus Parapenaeus longirostris Aristeus antennatus Dinámica de poblaciones |
| Sumario: | The knowledge on the age structure in exploited crustacean populations is essential not only to undertand their population dynamics but also for an effective stock assessment and management. In the present work, a novel methodology based on the quantification of the agepigment lipofuscin has been used for crustacean ageing became the difficulties found when trying to obtain reliable age estimates from conventional methods (either from studies on cultured specimens, tagging and recapture experiments, or size-frequency data from wild oppulations). Lipofuscin is an autofluorescent lipopigment that it is progressively accumulated with age in secondary lysosomes in post-mytotic tissues as a result of a cellular metabolism. A quantitative study of the lipofuscin content has been carried aut in known-age, pondreared prawns Marsupenaeus japonicus in order to assess the applicability of the lipofuscin method as an estimator of the physiological age in penaeids. Furthermore, the population age structure of two economically important species off the spanish waters, deep-water rose shrimp Parapenaeus longirotris and deep-water red 0000000shrimp Aristeus antennatus, was estimated from lipofuscin analysis. Quantification of lipofuscin content was carried out from brains´ histological sections, specifically from the olfactory lobe cell mass (OLCM), by fluorescence microscopy and image analysis techniques. Three differents lipofuscin measurements were recorded: % area fraction, granule density (nº gran./100µm²) and mean granule size (µm). In M. japonicus, lipofuscin levels increased significatly with age, and the accumulation rate with age showing sex-dependent. The relationship between age and lipofuscin concentration (area fraction and granule density) was best described by a seasonalized von Bertalanffy growth function, since the accumulation rate of the pigment dramatically slowed down, probably as a result of a reduced seasonal metabolism. In P. longirostris and A. antennatus, the relationship between lipofuscin concentration and size (expressed as carapace length) was best fitted to a linear model. A modal progression analysis of both size- and lipofuscin concentration-frequency distributions showed that the age structure of the population was better resolved from lipofuscin concentration-frequency data than it was from length .frequency data. The results confirm the potential of the lipofuscin method in the estimation of the physiological age in penaeids and suggest that the application of this methodology can be useful in studies on age structure in wild populations. |
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