Characterization of the lamin analogue NMCP2 in the monocot Allium cepa

26 p.-3 fig.-3 fig. supl.-1 tab. supl.

Detalles Bibliográficos
Autores: Ciska, Malgorzata, Masuda, Kiyoshi, Moreno Díaz de la Espina, Susana
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/159633
Acceso en línea:http://hdl.handle.net/10261/159633
Access Level:acceso abierto
Palabra clave:Allium cepa NMCP2 and NMCP1 proteins
Bioinformatic analysis
Immunofluorescence and immunoelectronmicroscopy
Phylogenetic analysis
Plant lamina
Protein analysis
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spelling Characterization of the lamin analogue NMCP2 in the monocot Allium cepaCiska, MalgorzataMasuda, KiyoshiMoreno Díaz de la Espina, SusanaAllium cepa NMCP2 and NMCP1 proteinsBioinformatic analysisImmunofluorescence and immunoelectronmicroscopyPhylogenetic analysisPlant laminaProtein analysis26 p.-3 fig.-3 fig. supl.-1 tab. supl.Nuclear lamina organization is similar in metazoan and plants though the latter lack orthologs of lamins, the main components of the metazoan lamina. Current evidence suggests that Nuclear Matrix Constituent Proteins (NMCPs) are the lamin analogues in plants as these proteins share several key features: higher-order secondary structure and domain layout, subnuclear distribution, and involvement in the regulation of nuclear shape and size, as well as in higher-order chromatin organization. Previously, we studied the NMCP family in flowering plants (angiosperms), in which it comprises two phylogenetic groups: NMCP1 and NMCP2. At present, in silico information about NMCP proteins in embryophytes is relatively advanced, though very few proteins, most of them of the NMCP1 type, have been extensively studied in vivo. We previously characterized the NCMP1 protein in the monocot Allium cepa. Here, we report the key features of a second protein of this species NMCP2, which presents a conserved sequence and domain layout. Immunofluorescence and immunoelectronmicroscopy evidence co-localization of endogenous AcNMCP2 and AcNMCP1 in the lamina, while Western blotting and immunoconfocal microscopy reveal a similar pattern of expression and distribution of both NMCP proteins in different root tissues. Our results provide novel insight about endogenous NMCP2-type proteins and complete the characterization of the NMCP family in A. cepa, thus advancing the current understanding of these structural proteins constituting the plant lamina.This work was supported by the Spanish Ministry of Science and Innovation [BFU2010-15900] and the Spanish National Research Council (CSIC) [PIE 201020E019].Peer reviewedSpringer NatureMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/159633reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1007/s00412-017-0649-2Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1596332026-05-22T06:33:51Z
dc.title.none.fl_str_mv Characterization of the lamin analogue NMCP2 in the monocot Allium cepa
title Characterization of the lamin analogue NMCP2 in the monocot Allium cepa
spellingShingle Characterization of the lamin analogue NMCP2 in the monocot Allium cepa
Ciska, Malgorzata
Allium cepa NMCP2 and NMCP1 proteins
Bioinformatic analysis
Immunofluorescence and immunoelectronmicroscopy
Phylogenetic analysis
Plant lamina
Protein analysis
title_short Characterization of the lamin analogue NMCP2 in the monocot Allium cepa
title_full Characterization of the lamin analogue NMCP2 in the monocot Allium cepa
title_fullStr Characterization of the lamin analogue NMCP2 in the monocot Allium cepa
title_full_unstemmed Characterization of the lamin analogue NMCP2 in the monocot Allium cepa
title_sort Characterization of the lamin analogue NMCP2 in the monocot Allium cepa
dc.creator.none.fl_str_mv Ciska, Malgorzata
Masuda, Kiyoshi
Moreno Díaz de la Espina, Susana
author Ciska, Malgorzata
author_facet Ciska, Malgorzata
Masuda, Kiyoshi
Moreno Díaz de la Espina, Susana
author_role author
author2 Masuda, Kiyoshi
Moreno Díaz de la Espina, Susana
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Allium cepa NMCP2 and NMCP1 proteins
Bioinformatic analysis
Immunofluorescence and immunoelectronmicroscopy
Phylogenetic analysis
Plant lamina
Protein analysis
topic Allium cepa NMCP2 and NMCP1 proteins
Bioinformatic analysis
Immunofluorescence and immunoelectronmicroscopy
Phylogenetic analysis
Plant lamina
Protein analysis
description 26 p.-3 fig.-3 fig. supl.-1 tab. supl.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/159633
url http://hdl.handle.net/10261/159633
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1007/s00412-017-0649-2
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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