Transcriptomic analysis of calcium remodeling in colorectal cancer

This article belongs to the Special Issue Latest Advances in Colorectal Cancer Research: Molecular Mechanisms and Therapies.

Detalles Bibliográficos
Autores: Pérez-Riesgo, Enrique, Gutiérrez, Lucía G., Ubierna, Daniel, Acedo, Alberto, Moyer, Mary P., Núñez, Lucía, Villalobos, Carlos
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/158104
Acceso en línea:http://hdl.handle.net/10261/158104
Access Level:acceso abierto
Palabra clave:RNA-sequencing
Transcriptomics
Ca2+ remodeling
Colorectal cancer
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spelling Transcriptomic analysis of calcium remodeling in colorectal cancerPérez-Riesgo, EnriqueGutiérrez, Lucía G.Ubierna, DanielAcedo, AlbertoMoyer, Mary P.Núñez, LucíaVillalobos, CarlosRNA-sequencingTranscriptomicsCa2+ remodelingColorectal cancerThis article belongs to the Special Issue Latest Advances in Colorectal Cancer Research: Molecular Mechanisms and Therapies.Colorectal cancer (CRC) cells undergo the remodeling of intracellular Ca homeostasis, which contributes to cancer hallmarks such as enhanced proliferation, invasion and survival. Ca remodeling includes critical changes in store-operated Ca entry (SOCE) and Ca store content. Some changes have been investigated at the molecular level. However, since nearly 100 genes are involved in intracellular Ca transport, a comprehensive view of Ca remodeling in CRC is lacking. We have used Next Generation Sequencing (NGS) to investigate differences in expression of 77 selected gene transcripts involved in intracellular Ca transport in CRC. To this end, mRNA from normal human colonic NCM460 cells and human colon cancer HT29 cells was isolated and used as a template for transcriptomic sequencing and expression analysis using Ion Torrent technology. After data transformation and filtering, exploratory analysis revealed that both cell types were well segregated. In addition, differential gene expression using R and bioconductor packages show significant differences in expression of selected voltage-operated Ca channels and store-operated Ca entry players, transient receptor potential (TRP) channels, Ca release channels, Ca pumps, Na/Ca exchanger isoforms and genes involved in mitochondrial Ca transport. These data provide the first comprehensive transcriptomic analysis of Ca remodeling in CRC.This work was supported by grants BFU2015-70131-R from Ministry of Economy and Competitivity of Spain to Carlos Villalobos and VA145U13. from Regional Government of Castilla y León, Spain to Lucía Núñez. Enrique Pérez-Riesgo is supported by a fellowship from the Spanish Association Against Cancer (AECC). Daniel Ubierna is supported by predoctoral fellowships from Junta de Castilla y León, Spain and the EU Social Fund. Lucía G. Gutiérrez is supported by a predoctoral fellowship from the University of Valladolid, SpainWe acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI).Peer ReviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Asociación Española Contra el CáncerEuropean CommissionJunta de Castilla y LeónUniversidad de ValladolidConsejo Superior de Investigaciones Científicas (España)CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720172017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/158104reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-70131-Rhttp://dx.doi.org/10.3390/ijms18050922Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1581042026-05-22T06:33:51Z
dc.title.none.fl_str_mv Transcriptomic analysis of calcium remodeling in colorectal cancer
title Transcriptomic analysis of calcium remodeling in colorectal cancer
spellingShingle Transcriptomic analysis of calcium remodeling in colorectal cancer
Pérez-Riesgo, Enrique
RNA-sequencing
Transcriptomics
Ca2+ remodeling
Colorectal cancer
title_short Transcriptomic analysis of calcium remodeling in colorectal cancer
title_full Transcriptomic analysis of calcium remodeling in colorectal cancer
title_fullStr Transcriptomic analysis of calcium remodeling in colorectal cancer
title_full_unstemmed Transcriptomic analysis of calcium remodeling in colorectal cancer
title_sort Transcriptomic analysis of calcium remodeling in colorectal cancer
dc.creator.none.fl_str_mv Pérez-Riesgo, Enrique
Gutiérrez, Lucía G.
Ubierna, Daniel
Acedo, Alberto
Moyer, Mary P.
Núñez, Lucía
Villalobos, Carlos
author Pérez-Riesgo, Enrique
author_facet Pérez-Riesgo, Enrique
Gutiérrez, Lucía G.
Ubierna, Daniel
Acedo, Alberto
Moyer, Mary P.
Núñez, Lucía
Villalobos, Carlos
author_role author
author2 Gutiérrez, Lucía G.
Ubierna, Daniel
Acedo, Alberto
Moyer, Mary P.
Núñez, Lucía
Villalobos, Carlos
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Asociación Española Contra el Cáncer
European Commission
Junta de Castilla y León
Universidad de Valladolid
Consejo Superior de Investigaciones Científicas (España)
CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv RNA-sequencing
Transcriptomics
Ca2+ remodeling
Colorectal cancer
topic RNA-sequencing
Transcriptomics
Ca2+ remodeling
Colorectal cancer
description This article belongs to the Special Issue Latest Advances in Colorectal Cancer Research: Molecular Mechanisms and Therapies.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/158104
url http://hdl.handle.net/10261/158104
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-70131-R
http://dx.doi.org/10.3390/ijms18050922
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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