Peripheral nervous system effects in the PS19 tau transgenic mouse model of tauopathy

It is well known that transgenic mice overexpressing human tau protein with P301S mutation driven by the mouse prion protein promoter show clasping and limb retraction, hunched back and paralysis, followed by inability to feed that results in death around 12 months of age. To understand these motor...

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Detalles Bibliográficos
Autores: Merchán-Rubira, Jesús, Sebastián-Serrano, Álvaro, Diaz-Hernandez, Miguel, Ávila, Jesús, Hernández Pérez, Félix
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/717608
Acceso en línea:http://hdl.handle.net/10486/717608
https://dx.doi.org/10.1016/j.neulet.2019.01.031
Access Level:acceso abierto
Palabra clave:Neurodegeneration
sciatic nerve
tauopathies
Biología y Biomedicina / Biología
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spelling Peripheral nervous system effects in the PS19 tau transgenic mouse model of tauopathyMerchán-Rubira, JesúsSebastián-Serrano, ÁlvaroDiaz-Hernandez, MiguelÁvila, JesúsHernández Pérez, FélixNeurodegenerationsciatic nervetauopathiesBiología y Biomedicina / BiologíaIt is well known that transgenic mice overexpressing human tau protein with P301S mutation driven by the mouse prion protein promoter show clasping and limb retraction, hunched back and paralysis, followed by inability to feed that results in death around 12 months of age. To understand these motor deficits, we have carried out rotarod tests on PS19 line and demonstrated how they worsened during aging. Then, we have analyzed if these phenotypic characteristics correlate with sciatic nerve degeneration. We first demonstrated by western blot and immunohistochemistry that the sciatic nerve expresses the transgenic tau protein; then, electron microscopy studies showed alterations in myelin, mainly a detachment of myelin lamellae at Schmidt-Lanterman clefts. Similar motor deficits and myelin alterations have been previously reported in tau knockout and overexpressing transgenic mice; taking into account that PS19 model is widely used to study tauopathies, we suggest that analyzing the expression of transgenic tau protein and myelin abnormalities in the sciatic nerve should be considered when studying some features as motor performance or survivalThis study was funded by grants from Spanish Ministry of Economy and Competitiveness (BFU2016-77885-P), Comunidad de Madrid co-financed with the Structural Funds of the European Union (S2017/BMD-3700 (NEUROMETAB-CM)), Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED, ISCIII), and from an institutional grant from the Fundación R. Areces . J M-R has a fellowship from Fundación La Caixa.ElsevierFacultad de Ciencias20192019-04-17research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/717608https://dx.doi.org/10.1016/j.neulet.2019.01.031reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7176082026-06-23T12:46:27Z
dc.title.none.fl_str_mv Peripheral nervous system effects in the PS19 tau transgenic mouse model of tauopathy
title Peripheral nervous system effects in the PS19 tau transgenic mouse model of tauopathy
spellingShingle Peripheral nervous system effects in the PS19 tau transgenic mouse model of tauopathy
Merchán-Rubira, Jesús
Neurodegeneration
sciatic nerve
tauopathies
Biología y Biomedicina / Biología
title_short Peripheral nervous system effects in the PS19 tau transgenic mouse model of tauopathy
title_full Peripheral nervous system effects in the PS19 tau transgenic mouse model of tauopathy
title_fullStr Peripheral nervous system effects in the PS19 tau transgenic mouse model of tauopathy
title_full_unstemmed Peripheral nervous system effects in the PS19 tau transgenic mouse model of tauopathy
title_sort Peripheral nervous system effects in the PS19 tau transgenic mouse model of tauopathy
dc.creator.none.fl_str_mv Merchán-Rubira, Jesús
Sebastián-Serrano, Álvaro
Diaz-Hernandez, Miguel
Ávila, Jesús
Hernández Pérez, Félix
author Merchán-Rubira, Jesús
author_facet Merchán-Rubira, Jesús
Sebastián-Serrano, Álvaro
Diaz-Hernandez, Miguel
Ávila, Jesús
Hernández Pérez, Félix
author_role author
author2 Sebastián-Serrano, Álvaro
Diaz-Hernandez, Miguel
Ávila, Jesús
Hernández Pérez, Félix
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Facultad de Ciencias
dc.subject.none.fl_str_mv Neurodegeneration
sciatic nerve
tauopathies
Biología y Biomedicina / Biología
topic Neurodegeneration
sciatic nerve
tauopathies
Biología y Biomedicina / Biología
description It is well known that transgenic mice overexpressing human tau protein with P301S mutation driven by the mouse prion protein promoter show clasping and limb retraction, hunched back and paralysis, followed by inability to feed that results in death around 12 months of age. To understand these motor deficits, we have carried out rotarod tests on PS19 line and demonstrated how they worsened during aging. Then, we have analyzed if these phenotypic characteristics correlate with sciatic nerve degeneration. We first demonstrated by western blot and immunohistochemistry that the sciatic nerve expresses the transgenic tau protein; then, electron microscopy studies showed alterations in myelin, mainly a detachment of myelin lamellae at Schmidt-Lanterman clefts. Similar motor deficits and myelin alterations have been previously reported in tau knockout and overexpressing transgenic mice; taking into account that PS19 model is widely used to study tauopathies, we suggest that analyzing the expression of transgenic tau protein and myelin abnormalities in the sciatic nerve should be considered when studying some features as motor performance or survival
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-04-17
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/717608
https://dx.doi.org/10.1016/j.neulet.2019.01.031
url http://hdl.handle.net/10486/717608
https://dx.doi.org/10.1016/j.neulet.2019.01.031
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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