Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations

[Introduction]: Monitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care. Due to their immunophenotypic heterogeneity and biological plasticity, analysis of IMC populations typically requires large panels of markers. Curre...

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Autores: Pan, Kyra van der, Khatri, Indu, Jager, Anniek L. de, Louis, Alesha, Kassem, Sara, Naber, Brigitta A. E., Laat, Inge F. de, Hameetman, Marjolijn, Comans, Suzanne, Orfao, Alberto, Dongen, J. J. M. van, Díez, Paula, Teodosio, Cristina
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/348256
Acesso em linha:http://hdl.handle.net/10261/348256
Access Level:Acceso aberto
Palavra-chave:Spectral flow cytometry
Myeloid cells
Immunophenotyping
cyTOF
Mass cytometry
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network_name_str España
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dc.title.none.fl_str_mv Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations
title Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations
spellingShingle Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations
Pan, Kyra van der
Spectral flow cytometry
Myeloid cells
Immunophenotyping
cyTOF
Mass cytometry
title_short Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations
title_full Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations
title_fullStr Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations
title_full_unstemmed Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations
title_sort Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations
dc.creator.none.fl_str_mv Pan, Kyra van der
Khatri, Indu
Jager, Anniek L. de
Louis, Alesha
Kassem, Sara
Naber, Brigitta A. E.
Laat, Inge F. de
Hameetman, Marjolijn
Comans, Suzanne
Orfao, Alberto
Dongen, J. J. M. van
Díez, Paula
Teodosio, Cristina
author Pan, Kyra van der
author_facet Pan, Kyra van der
Khatri, Indu
Jager, Anniek L. de
Louis, Alesha
Kassem, Sara
Naber, Brigitta A. E.
Laat, Inge F. de
Hameetman, Marjolijn
Comans, Suzanne
Orfao, Alberto
Dongen, J. J. M. van
Díez, Paula
Teodosio, Cristina
author_role author
author2 Khatri, Indu
Jager, Anniek L. de
Louis, Alesha
Kassem, Sara
Naber, Brigitta A. E.
Laat, Inge F. de
Hameetman, Marjolijn
Comans, Suzanne
Orfao, Alberto
Dongen, J. J. M. van
Díez, Paula
Teodosio, Cristina
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Research Council
European Commission
Instituto de Salud Carlos III
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Spectral flow cytometry
Myeloid cells
Immunophenotyping
cyTOF
Mass cytometry
topic Spectral flow cytometry
Myeloid cells
Immunophenotyping
cyTOF
Mass cytometry
description [Introduction]: Monitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care. Due to their immunophenotypic heterogeneity and biological plasticity, analysis of IMC populations typically requires large panels of markers. Currently, two cytometry-based techniques allow for the simultaneous detection of ≥40 markers: spectral flow cytometry (SFC) and mass cytometry (MC). However, little is known about the comparability of SFC and MC in studying IMC populations.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/348256
url http://hdl.handle.net/10261/348256
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/695655
info:eu-repo/grantAgreement/EC/H2020/101034371
Pan, Kyra van der; Khatri, Indu; Jager, Anniek L. de; Louis, Alesha; Kassem, Sara; Naber, Brigitta A. E.; Laat, Inge F. de; Hameetman, Marjolijn; Comans, Suzanne; Orfao, Alberto; Dongen, J. J. M. van; Díez, Paula; Teodosio, Cristina; 2023; DataSheet_1_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.docx [Dataset]; Frontiers Media; https://doi.org/10.3389/fimmu.2023.1191992.s001
Pan, Kyra van der; Khatri, Indu; Jager, Anniek L. de; Louis, Alesha; Kassem, Sara; Naber, Brigitta A. E.; Laat, Inge F. de; Hameetman, Marjolijn; Comans, Suzanne; Orfao, Alberto; Dongen, J. J. M. van; Díez, Paula; Teodosio, Cristina; 2023; Image_1_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]; Frontiers Media; https://doi.org/10.3389/fimmu.2023.1191992.s002
Pan, Kyra van der; Khatri, Indu; Jager, Anniek L. de; Louis, Alesha; Kassem, Sara; Naber, Brigitta A. E.; Laat, Inge F. de; Hameetman, Marjolijn; Comans, Suzanne; Orfao, Alberto; Dongen, J. J. M. van; Díez, Paula; Teodosio, Cristina; 2023; Image_2_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]; Frontiers Media; https://doi.org/10.3389/fimmu.2023.1191992.s003
Pan, Kyra van der; Khatri, Indu; Jager, Anniek L. de; Louis, Alesha; Kassem, Sara; Naber, Brigitta A. E.; Laat, Inge F. de; Hameetman, Marjolijn; Comans, Suzanne; Orfao, Alberto; Dongen, J. J. M. van; Díez, Paula; Teodosio, Cristina; 2023; Image_3_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]; Frontiers Media; https://doi.org/10.3389/fimmu.2023.1191992.s004
Pan, Kyra van der; Khatri, Indu; Jager, Anniek L. de; Louis, Alesha; Kassem, Sara; Naber, Brigitta A. E.; Laat, Inge F. de; Hameetman, Marjolijn; Comans, Suzanne; Orfao, Alberto; Dongen, J. J. M. van; Díez, Paula; Teodosio, Cristina; 2023; Image_4_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]; Frontiers Media; https://doi.org/10.3389/fimmu.2023.1191992.s005
Pan, Kyra van der; Khatri, Indu; Jager, Anniek L. de; Louis, Alesha; Kassem, Sara; Naber, Brigitta A. E.; Laat, Inge F. de; Hameetman, Marjolijn; Comans, Suzanne; Orfao, Alberto; Dongen, J. J. M. van; Díez, Paula; Teodosio, Cristina; 2023; Image_5_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]; Frontiers Media; https://doi.org/10.3389/fimmu.2023.1191992.s006
Pan, Kyra van der; Khatri, Indu; Jager, Anniek L. de; Louis, Alesha; Kassem, Sara; Naber, Brigitta A. E.; Laat, Inge F. de; Hameetman, Marjolijn; Comans, Suzanne; Orfao, Alberto; Dongen, J. J. M. van; Díez, Paula; Teodosio, Cristina; 2023; Image_6_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]; Frontiers Media; https://doi.org/10.3389/fimmu.2023.1191992.s007
https://doi.org/10.3389/fimmu.2023.1191992
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
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spelling Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populationsPan, Kyra van derKhatri, InduJager, Anniek L. deLouis, AleshaKassem, SaraNaber, Brigitta A. E.Laat, Inge F. deHameetman, MarjolijnComans, SuzanneOrfao, AlbertoDongen, J. J. M. vanDíez, PaulaTeodosio, CristinaSpectral flow cytometryMyeloid cellsImmunophenotypingcyTOFMass cytometry[Introduction]: Monitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care. Due to their immunophenotypic heterogeneity and biological plasticity, analysis of IMC populations typically requires large panels of markers. Currently, two cytometry-based techniques allow for the simultaneous detection of ≥40 markers: spectral flow cytometry (SFC) and mass cytometry (MC). However, little is known about the comparability of SFC and MC in studying IMC populations.[Methods]: We evaluated the performance of two SFC and MC panels, which contained 21 common markers, for the identification and subsetting of blood IMC populations. Based on unsupervised clustering analysis, we systematically identified 24 leukocyte populations, including 21 IMC subsets, regardless of the cytometry technique.[Results]: Overall, comparable results were observed between the two technologies regarding the relative distribution of these cell populations and the staining resolution of individual markers (Pearson’s ρ=0.99 and 0.55, respectively). However, minor differences were observed between the two techniques regarding intra-measurement variability (median coefficient of variation of 42.5% vs. 68.0% in SFC and MC, respectively; p<0.0001) and reproducibility, which were most likely due to the significantly longer acquisition times (median 16 min vs. 159 min) and lower recovery rates (median 53.1% vs. 26.8%) associated with SFC vs. MC.[Discussion]: Altogether, our results show a good correlation between SFC and MC for the identification, enumeration and characterization of IMC in blood, based on large panels (>20) of antibody reagents.The work here presented was funded by the European Research Council under the European Union’s Horizon 2020 Research and Innovation Programme with an ERC Advanced Grant (ERC-2015-AdG 695655, TiMaScan). CT is the recipient of an USAL4EXCELLENCE fellowship, funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 101034371. PD is supported by a Sara Borrell postdoctoral fellowship (Institute of Health Carlos III, Spanish Government, CD22/00009).Peer reviewedFrontiers MediaEuropean Research CouncilEuropean CommissionInstituto de Salud Carlos IIIConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/348256reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/695655info:eu-repo/grantAgreement/EC/H2020/101034371Pan, Kyra van der; Khatri, Indu; Jager, Anniek L. de; Louis, Alesha; Kassem, Sara; Naber, Brigitta A. E.; Laat, Inge F. de; Hameetman, Marjolijn; Comans, Suzanne; Orfao, Alberto; Dongen, J. J. M. van; Díez, Paula; Teodosio, Cristina; 2023; DataSheet_1_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.docx [Dataset]; Frontiers Media; https://doi.org/10.3389/fimmu.2023.1191992.s001Pan, Kyra van der; Khatri, Indu; Jager, Anniek L. de; Louis, Alesha; Kassem, Sara; Naber, Brigitta A. E.; Laat, Inge F. de; Hameetman, Marjolijn; Comans, Suzanne; Orfao, Alberto; Dongen, J. J. M. van; Díez, Paula; Teodosio, Cristina; 2023; Image_1_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]; Frontiers Media; https://doi.org/10.3389/fimmu.2023.1191992.s002Pan, Kyra van der; Khatri, Indu; Jager, Anniek L. de; Louis, Alesha; Kassem, Sara; Naber, Brigitta A. E.; Laat, Inge F. de; Hameetman, Marjolijn; Comans, Suzanne; Orfao, Alberto; Dongen, J. J. M. van; Díez, Paula; Teodosio, Cristina; 2023; Image_2_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]; Frontiers Media; https://doi.org/10.3389/fimmu.2023.1191992.s003Pan, Kyra van der; Khatri, Indu; Jager, Anniek L. de; Louis, Alesha; Kassem, Sara; Naber, Brigitta A. E.; Laat, Inge F. de; Hameetman, Marjolijn; Comans, Suzanne; Orfao, Alberto; Dongen, J. J. M. van; Díez, Paula; Teodosio, Cristina; 2023; Image_3_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]; Frontiers Media; https://doi.org/10.3389/fimmu.2023.1191992.s004Pan, Kyra van der; Khatri, Indu; Jager, Anniek L. de; Louis, Alesha; Kassem, Sara; Naber, Brigitta A. E.; Laat, Inge F. de; Hameetman, Marjolijn; Comans, Suzanne; Orfao, Alberto; Dongen, J. J. M. van; Díez, Paula; Teodosio, Cristina; 2023; Image_4_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]; Frontiers Media; https://doi.org/10.3389/fimmu.2023.1191992.s005Pan, Kyra van der; Khatri, Indu; Jager, Anniek L. de; Louis, Alesha; Kassem, Sara; Naber, Brigitta A. E.; Laat, Inge F. de; Hameetman, Marjolijn; Comans, Suzanne; Orfao, Alberto; Dongen, J. J. M. van; Díez, Paula; Teodosio, Cristina; 2023; Image_5_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]; Frontiers Media; https://doi.org/10.3389/fimmu.2023.1191992.s006Pan, Kyra van der; Khatri, Indu; Jager, Anniek L. de; Louis, Alesha; Kassem, Sara; Naber, Brigitta A. E.; Laat, Inge F. de; Hameetman, Marjolijn; Comans, Suzanne; Orfao, Alberto; Dongen, J. J. M. van; Díez, Paula; Teodosio, Cristina; 2023; Image_6_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif [Dataset]; Frontiers Media; https://doi.org/10.3389/fimmu.2023.1191992.s007https://doi.org/10.3389/fimmu.2023.1191992Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3482562026-05-22T06:33:51Z
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