Isolated White Lupin Proteins Beneficially Modulate the Intestinal Microbiota Composition in Rats

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Detalles Bibliográficos
Autores: Rubio, Luis A., Chiesa, Giulia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/379529
Acceso en línea:http://hdl.handle.net/10261/379529
Access Level:acceso abierto
Palabra clave:Casein
Intestinal microbiota composition
Lactalbumin
Lupinus albus
Protein isolates
Rat
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spelling Isolated White Lupin Proteins Beneficially Modulate the Intestinal Microbiota Composition in RatsRubio, Luis A.Chiesa, GiuliaCaseinIntestinal microbiota compositionLactalbuminLupinus albusProtein isolatesRatThis is an early access version, the complete PDF, HTML, and XML versions will be available soon.[Background] Previous work has shown that the mostly beneficial modulation of intestinal microbiota generally found with legume-based diets is likely to be due, at least in part, to their constituent protein components. Objectives: The faecal microbiota composition was studied in rats fed diets differing only in their constituent proteins. Methods: Rats (n = 10/group) were fed for 28 days diets based in milk [(lactalbumin (LA), casein (CAS)], or white lupin (Lupinus albus) protein isolate (LPI). Results: Significant differences among the three groups in bacteria composition and functionality were found by both qPCR and Illumina sequencing analysis. Significant (p < 0.01) differences were found by ANOSIM and Discriminant Analysis among groups at the family, genus and species levels in both microbiota composition and functionality. A number of groups able to explain the differences between animal (casein, lactalbumin) and lupin proteins were revealed by LEfSe and PCA analysis. Specifically, feeding the CAS diet resulted in lower Bifidobacteria and Lactobacilli compared to the other diets, and the LPI diet gave place to lower Enterobacteria than CAS, and lower Escherichia/Shigella than LA and CAS. Differences in the LA group were attributable to Bifidobacterium spp., Collinsella spp. (in particular C. stercoris), Bacteroides spp., Eubacterium spp. (in particular E. dolichum), Roseburia spp. (in particular R. faecis), and Oscillospira spp. In the case of the CAS group, the organisms were Parabacteroides spp., Blautia spp., Enterobacteriaceae spp., Turicibacter spp., species from Christenellaceae, species from Alphaproteobacteria and Mogibacteriaceae, Coprobacillus spp. and Dorea spp. In the case of the LPI group, the organisms were Lactobacillus spp. (Lactobacillus spp. and L. reuteri), species from Clostridiaceae, species from Peptostreptococcaceae, species from Erysipelotrichaceae, and Adlercreutzia spp. Conclusions: Based on the results obtained, LPI is likely to beneficially modulate the intestinal microbiota composition in rats. Additionally, LA-based diet was associated to a healthier microbiota composition than CAS, although the CAS diet also modulated the intestinal microbiota to a composition compatible with improved bowel movement frequency and lipid metabolism.This research was funded by the Spanish CSIC, the Ministry of Science and Innovation (project RTI2018-100934-B-I00), and INIA (project no. RTA 03-202). The work was also partially supported by the Fondo Europeo de Desarrollo Regional (FEDER), Fondo Social Europeo (FSE), European Union’s Horizon 2020 research and innovation programme under the ERA-Net Cofund action no. 727565 and funds from the European Union (Fifth Framework Programme, Quality of Life and Management of Living Resources programme, Healthy-Profood QLRT 2001-2235).Peer reviewedMultidisciplinary Digital Publishing InstituteEuropean CommissionAgencia Estatal de Investigación (España)Rubio, Luis A. [0000-0002-7555-2913]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2025202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/379529reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/727565info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-100934-B-I00Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3795292026-05-22T06:33:51Z
dc.title.none.fl_str_mv Isolated White Lupin Proteins Beneficially Modulate the Intestinal Microbiota Composition in Rats
title Isolated White Lupin Proteins Beneficially Modulate the Intestinal Microbiota Composition in Rats
spellingShingle Isolated White Lupin Proteins Beneficially Modulate the Intestinal Microbiota Composition in Rats
Rubio, Luis A.
Casein
Intestinal microbiota composition
Lactalbumin
Lupinus albus
Protein isolates
Rat
title_short Isolated White Lupin Proteins Beneficially Modulate the Intestinal Microbiota Composition in Rats
title_full Isolated White Lupin Proteins Beneficially Modulate the Intestinal Microbiota Composition in Rats
title_fullStr Isolated White Lupin Proteins Beneficially Modulate the Intestinal Microbiota Composition in Rats
title_full_unstemmed Isolated White Lupin Proteins Beneficially Modulate the Intestinal Microbiota Composition in Rats
title_sort Isolated White Lupin Proteins Beneficially Modulate the Intestinal Microbiota Composition in Rats
dc.creator.none.fl_str_mv Rubio, Luis A.
Chiesa, Giulia
author Rubio, Luis A.
author_facet Rubio, Luis A.
Chiesa, Giulia
author_role author
author2 Chiesa, Giulia
author2_role author
dc.contributor.none.fl_str_mv European Commission
Agencia Estatal de Investigación (España)
Rubio, Luis A. [0000-0002-7555-2913]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Casein
Intestinal microbiota composition
Lactalbumin
Lupinus albus
Protein isolates
Rat
topic Casein
Intestinal microbiota composition
Lactalbumin
Lupinus albus
Protein isolates
Rat
description This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/379529
url http://hdl.handle.net/10261/379529
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/727565
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-100934-B-I00
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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repository.mail.fl_str_mv
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