Conidia production of the entomopathogenic fungus Beauveria bassiana using packed-bed bioreactor
This work presents the scale-up of the conidia production of the entomopathogenic fungus Beauveria bassiana using two different wastes, coupled with concentration and virulence tests of the produced conidia against the pest Tenebrio molitor. Beauveria bassiana CECT 20374 was used in solid state ferm...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:274808 |
| Acceso en línea: | https://ddd.uab.cat/record/274808 https://dx.doi.org/urn:doi:10.1016/j.jenvman.2023.118059 |
| Access Level: | acceso abierto |
| Palabra clave: | Solid state fermentation Substrate biodegradability Virulence Beauveria bassiana Tenebrio molitor |
| Sumario: | This work presents the scale-up of the conidia production of the entomopathogenic fungus Beauveria bassiana using two different wastes, coupled with concentration and virulence tests of the produced conidia against the pest Tenebrio molitor. Beauveria bassiana CECT 20374 was used in solid state fermentation (SSF) operating under batch strategy. Two substrates with different biodegradability (rice husk and beer draff) were tested, successfully scaling from 1.5 L to 22 L bioreactors. Higher conidia production was reached using beer draff as substrate (2.5 × 109 and 6.0 × 108 conidia g-1 dry matter in 1.5 and 22 L reactors respectively) highlighting air free porosity relevance as scale-up parameter. Concentration and dose-response tests against larvae and adult Tenebrio molitor were performed to compare strain CECT 20374 with control strain KVL 13-39 (a B. bassiana strain previously tested against T. molitor). Virulence effect of the 22 L fermentation product of strain CECT using rice husk or beer draff was tested against T. molitor adult stage. However, quality loses between conidia produced in agar plates and fermented products were observed (from 75 to 80% mortality in plates to 40% in rice husk and 50-60% in beer draff fermented products respectively). The differences between plate and fermented samples also indicated fermentation process, extraction and conservation steps as possible causes for quality losses, highlighting the need to optimize them to maximize virulence maintenance. |
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