A new hormonal protocol supports early development of in vitro-produced embryos after transfer to anoestrus mares

The present study aimed to evaluate whether primed anoestrus mares are suitable recipients for embryos produced by intracytoplasmic sperm injection (ICSI). Anoestrus was confirmed in four mares and daily doses of oestradiol benzoate (6 mg in total) over 5 days were administered; after 3 days of rest...

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Detalles Bibliográficos
Autores: Muñoz García, Carmen C., Luis Calero, Marcos, Fernández Hernández, Pablo, Pizarro Hernández, Alba, Fernández González, Raúl, Gutiérrez Adán, Alfonso, Sánchez Calabuig, María Jesús, Cuervo Arango, Juan, González Fernández, Lauro, Macías García, Beatriz
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/109800
Acceso en línea:https://hdl.handle.net/20.500.14352/109800
Access Level:acceso abierto
Palabra clave:636.09
Anoestrus recipient
In vitro embryo
Mare
Transfer
Veterinaria
3109 Ciencias Veterinarias
Descripción
Sumario:The present study aimed to evaluate whether primed anoestrus mares are suitable recipients for embryos produced by intracytoplasmic sperm injection (ICSI). Anoestrus was confirmed in four mares and daily doses of oestradiol benzoate (6 mg in total) over 5 days were administered; after 3 days of rest, oral altrenogest was administered at 0.088 mg/kg and embryos (1 to 5 embryos per mare; 15 in total) were transferred 3.5 days after progesterone onset. Uterine lavage was conducted 48 h after transfer. The results revealed an 80% embryo recovery rate, and among the retrieved embryos, 67% showed evident intrauterine development. Hence, ICSI-derived embryos can be successfully transferred to primed anoestrus mares, but more studies are required to ensure further embryo development and foaling.