Multi-walled carbon nanotubes (NM401) induce ROS-mediated HPRT mutations in Chinese hamster lung fibroblasts

Although there is an important set of data showing potential genotoxic effects of nanomaterials (NMs) at the DNA (comet assay) and chromosome (micronucleus test) levels, few studies have been conducted to analyze their potential mutagenic effects at gene level. We have determined the ability of mult...

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Autores: Rubio Lorente, Laura|||0000-0002-2088-3803, El Yamani, Naouale|||0000-0001-5456-8230, Kažimirova, Alena, Dusinska, Maria|||0000-0003-1358-1652, Marcos Dauder, Ricardo|||0000-0001-7891-357X
Formato: artículo
Fecha de publicación:2016
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:308391
Acesso em linha:https://ddd.uab.cat/record/308391
https://dx.doi.org/urn:doi:10.1016/j.envres.2016.01.004
Access Level:acceso abierto
Palavra-chave:Genotoxicity
HPRT gene
Multi-walled carbon nanotubes
Ros
V79 cells
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spelling Multi-walled carbon nanotubes (NM401) induce ROS-mediated HPRT mutations in Chinese hamster lung fibroblastsRubio Lorente, Laura|||0000-0002-2088-3803El Yamani, Naouale|||0000-0001-5456-8230Kažimirova, AlenaDusinska, Maria|||0000-0003-1358-1652Marcos Dauder, Ricardo|||0000-0001-7891-357XGenotoxicityHPRT geneMulti-walled carbon nanotubesRosV79 cellsAlthough there is an important set of data showing potential genotoxic effects of nanomaterials (NMs) at the DNA (comet assay) and chromosome (micronucleus test) levels, few studies have been conducted to analyze their potential mutagenic effects at gene level. We have determined the ability of multi-walled carbon nanotubes (MWCNT, NM401), to induce mutations in the HPRT gene in Chinese hamster lung (V79) fibroblasts. NM401, characterized in the EU NanoGenotox project, were further studied within the EU Framework Programme Seven (FP7) project NANoREG. From the proliferation assay data we selected a dose-range of 0.12 to 12 mu g/cm(2) At these range we have been able to observe significant cellular uptake of MWCNT by using transmission electron microscopy (TEM), as well as a concentration-dependent induction of intracellular reactive oxygen species. In addition, a clear concentration-dependent increase in the induction of HPRT mutations was also observed. Data support a potential genotoxic/ carcinogenic risk associated with MWCNT exposure. 22016-01-0120162016-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/308391https://dx.doi.org/urn:doi:10.1016/j.envres.2016.01.004reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengEuropean Commission https://doi.org/10.13039/501100000780 310584European Commission https://doi.org/10.13039/501100000780 262163open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3083912026-06-06T12:50:31Z
dc.title.none.fl_str_mv Multi-walled carbon nanotubes (NM401) induce ROS-mediated HPRT mutations in Chinese hamster lung fibroblasts
title Multi-walled carbon nanotubes (NM401) induce ROS-mediated HPRT mutations in Chinese hamster lung fibroblasts
spellingShingle Multi-walled carbon nanotubes (NM401) induce ROS-mediated HPRT mutations in Chinese hamster lung fibroblasts
Rubio Lorente, Laura|||0000-0002-2088-3803
Genotoxicity
HPRT gene
Multi-walled carbon nanotubes
Ros
V79 cells
title_short Multi-walled carbon nanotubes (NM401) induce ROS-mediated HPRT mutations in Chinese hamster lung fibroblasts
title_full Multi-walled carbon nanotubes (NM401) induce ROS-mediated HPRT mutations in Chinese hamster lung fibroblasts
title_fullStr Multi-walled carbon nanotubes (NM401) induce ROS-mediated HPRT mutations in Chinese hamster lung fibroblasts
title_full_unstemmed Multi-walled carbon nanotubes (NM401) induce ROS-mediated HPRT mutations in Chinese hamster lung fibroblasts
title_sort Multi-walled carbon nanotubes (NM401) induce ROS-mediated HPRT mutations in Chinese hamster lung fibroblasts
dc.creator.none.fl_str_mv Rubio Lorente, Laura|||0000-0002-2088-3803
El Yamani, Naouale|||0000-0001-5456-8230
Kažimirova, Alena
Dusinska, Maria|||0000-0003-1358-1652
Marcos Dauder, Ricardo|||0000-0001-7891-357X
author Rubio Lorente, Laura|||0000-0002-2088-3803
author_facet Rubio Lorente, Laura|||0000-0002-2088-3803
El Yamani, Naouale|||0000-0001-5456-8230
Kažimirova, Alena
Dusinska, Maria|||0000-0003-1358-1652
Marcos Dauder, Ricardo|||0000-0001-7891-357X
author_role author
author2 El Yamani, Naouale|||0000-0001-5456-8230
Kažimirova, Alena
Dusinska, Maria|||0000-0003-1358-1652
Marcos Dauder, Ricardo|||0000-0001-7891-357X
author2_role author
author
author
author
dc.subject.none.fl_str_mv Genotoxicity
HPRT gene
Multi-walled carbon nanotubes
Ros
V79 cells
topic Genotoxicity
HPRT gene
Multi-walled carbon nanotubes
Ros
V79 cells
description Although there is an important set of data showing potential genotoxic effects of nanomaterials (NMs) at the DNA (comet assay) and chromosome (micronucleus test) levels, few studies have been conducted to analyze their potential mutagenic effects at gene level. We have determined the ability of multi-walled carbon nanotubes (MWCNT, NM401), to induce mutations in the HPRT gene in Chinese hamster lung (V79) fibroblasts. NM401, characterized in the EU NanoGenotox project, were further studied within the EU Framework Programme Seven (FP7) project NANoREG. From the proliferation assay data we selected a dose-range of 0.12 to 12 mu g/cm(2) At these range we have been able to observe significant cellular uptake of MWCNT by using transmission electron microscopy (TEM), as well as a concentration-dependent induction of intracellular reactive oxygen species. In addition, a clear concentration-dependent increase in the induction of HPRT mutations was also observed. Data support a potential genotoxic/ carcinogenic risk associated with MWCNT exposure.
publishDate 2016
dc.date.none.fl_str_mv 2
2016-01-01
2016
2016-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/308391
https://dx.doi.org/urn:doi:10.1016/j.envres.2016.01.004
url https://ddd.uab.cat/record/308391
https://dx.doi.org/urn:doi:10.1016/j.envres.2016.01.004
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 310584
European Commission https://doi.org/10.13039/501100000780 262163
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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