Mechanical unfolding of long human telomeric RNA (TERRA)

[EN] We report the first single molecule investigation of TERRA molecules. By using optical-tweezers and other biophysical techniques, we have found that long RNA constructions of up to 25 GGGUUA repeats form higher order structures comprised of single parallel G-quadruplex blocks, which unfold at l...

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Detalles Bibliográficos
Autores: Garavís, M., Bocanegra, R., Herrero-Galán, E., González, C., Villasante, A., Arias-Gonzalez, J. R.|||0000-0001-6802-0874
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/152715
Acceso en línea:https://riunet.upv.es/handle/10251/152715
Access Level:acceso abierto
Palabra clave:G-quadruplex
TERRA
Single-molecule
Optical tweezers
Unfolding
Stability
FISICA APLICADA
Descripción
Sumario:[EN] We report the first single molecule investigation of TERRA molecules. By using optical-tweezers and other biophysical techniques, we have found that long RNA constructions of up to 25 GGGUUA repeats form higher order structures comprised of single parallel G-quadruplex blocks, which unfold at lower forces than their DNA counterparts.