Widening the pH Activity Profile of a Fungal Laccase by Directed Evolution
Unnatural selection: A fungal laccase was tailored by directed evolution to be active at neutral/alkaline pH. After five generations, the final mutant showed a broader pH profile while retaining 50 to 80 % of its activity at neutral pH.
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2013 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/183480 |
| Acceso en línea: | http://hdl.handle.net/10261/183480 |
| Access Level: | acceso abierto |
| Palabra clave: | Laccase Saccharomyces cerevisiae Enzymes Directed evolution C‐terminal plug Alkaline pH |
| Sumario: | Unnatural selection: A fungal laccase was tailored by directed evolution to be active at neutral/alkaline pH. After five generations, the final mutant showed a broader pH profile while retaining 50 to 80 % of its activity at neutral pH. |
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