Singlet oxygen triggers chloroplast rupture and cell death in the zeaxanthin epoxidase defective mutant aba1 of Arabidopsis thaliana under high light stress

44 páginas, 6 figuras, 1 tabla. -- The definitive version is available at http://www.elsevier.com

Detalles Bibliográficos
Autores: Sánchez Corrionero, Álvaro, Sánchez Vicente, Inmaculada, González Pérez, Sergio, Corrales, Ascensión, Krieger-Liszkay, Anja, Lorenzo, Óscar, Arellano, Juan B.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/153694
Acceso en línea:http://hdl.handle.net/10261/153694
Access Level:acceso abierto
Palabra clave:AAA-ATPase
aba1
Chloroplast rupture
JAZ repressors
Cell death
Singlet oxygen
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spelling Singlet oxygen triggers chloroplast rupture and cell death in the zeaxanthin epoxidase defective mutant aba1 of Arabidopsis thaliana under high light stressSánchez Corrionero, ÁlvaroSánchez Vicente, InmaculadaGonzález Pérez, SergioCorrales, AscensiónKrieger-Liszkay, AnjaLorenzo, ÓscarArellano, Juan B.AAA-ATPaseaba1Chloroplast ruptureJAZ repressorsCell deathSinglet oxygen44 páginas, 6 figuras, 1 tabla. -- The definitive version is available at http://www.elsevier.comThe two Arabidopsis thaliana mutants, aba1 and max4, were previously identified as sharing a number of co-regulated genes with both the flu mutant and Arabidopsis cell suspension cultures exposed to high light (HL). On this basis, we investigated whether aba1 and max4 were generating high amounts of singlet oxygen (1O2) and activating 1O2-mediated cell death. Thylakoids of aba1 produced twice as much 1O2 as thylakoids of max4 and wild type (WT) plants when illuminated with strong red light. 1O2 was measured using the spin probe 2,2,6,6-tetramethyl-4-piperidone hydrochloride. 77-K chlorophyll fluorescence emission spectra of thylakoids revealed lower aggregation of the light harvesting complex II in aba1. This was rationalized as a loss of connectivity between photosystem II (PSII) units and as the main cause for the high yield of 1O2 generation in aba1. Up-regulation of the 1O2 responsive gene AAA-ATPase was only observed with statistical significant in aba1 under HL. Two early jasmonate (JA)-responsive genes, JAZ1 and JAZ5, encoding for two repressor proteins involved in the negative feedback regulation of JA signalling, were not up-regulated to the WT plant levels. Chloroplast aggregation followed by chloroplast rupture and eventual cell death was observed by confocal imaging of the fluorescence emission of leaf cells of transgenic aba1 plants expressing the chimeric fusion protein SSU-GFP. Cell death was not associated with direct 1O2 cytotoxicity in aba1, but rather with a delayed stress response. In contrast, max4 did not show evidence of 1O2-mediated cell death. In conclusion, aba1 may serve as an alternative model to other 1O2-overproducing mutants of Arabidopsis for investigating 1O2-mediated cell death.This work was supported by MINECO (AGL2013-41363-R, ERDF) and Junta de Castilla y León (CSI002A10-2 and CSI083U16, ERDF) to J.B.A., ERC.KBBE.2012.1.1-01 (EcoSeed-311840) to Ó.L and A. K.-L., and MINECO (BIO2014-57107-R) and Junta de Castilla y León (SA239U13 and SA093U16) to Ó.L.Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Junta de Castilla y LeónConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201720172017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/153694reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.jplph.2017.06.009Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1536942026-05-22T06:33:51Z
dc.title.none.fl_str_mv Singlet oxygen triggers chloroplast rupture and cell death in the zeaxanthin epoxidase defective mutant aba1 of Arabidopsis thaliana under high light stress
title Singlet oxygen triggers chloroplast rupture and cell death in the zeaxanthin epoxidase defective mutant aba1 of Arabidopsis thaliana under high light stress
spellingShingle Singlet oxygen triggers chloroplast rupture and cell death in the zeaxanthin epoxidase defective mutant aba1 of Arabidopsis thaliana under high light stress
Sánchez Corrionero, Álvaro
AAA-ATPase
aba1
Chloroplast rupture
JAZ repressors
Cell death
Singlet oxygen
title_short Singlet oxygen triggers chloroplast rupture and cell death in the zeaxanthin epoxidase defective mutant aba1 of Arabidopsis thaliana under high light stress
title_full Singlet oxygen triggers chloroplast rupture and cell death in the zeaxanthin epoxidase defective mutant aba1 of Arabidopsis thaliana under high light stress
title_fullStr Singlet oxygen triggers chloroplast rupture and cell death in the zeaxanthin epoxidase defective mutant aba1 of Arabidopsis thaliana under high light stress
title_full_unstemmed Singlet oxygen triggers chloroplast rupture and cell death in the zeaxanthin epoxidase defective mutant aba1 of Arabidopsis thaliana under high light stress
title_sort Singlet oxygen triggers chloroplast rupture and cell death in the zeaxanthin epoxidase defective mutant aba1 of Arabidopsis thaliana under high light stress
dc.creator.none.fl_str_mv Sánchez Corrionero, Álvaro
Sánchez Vicente, Inmaculada
González Pérez, Sergio
Corrales, Ascensión
Krieger-Liszkay, Anja
Lorenzo, Óscar
Arellano, Juan B.
author Sánchez Corrionero, Álvaro
author_facet Sánchez Corrionero, Álvaro
Sánchez Vicente, Inmaculada
González Pérez, Sergio
Corrales, Ascensión
Krieger-Liszkay, Anja
Lorenzo, Óscar
Arellano, Juan B.
author_role author
author2 Sánchez Vicente, Inmaculada
González Pérez, Sergio
Corrales, Ascensión
Krieger-Liszkay, Anja
Lorenzo, Óscar
Arellano, Juan B.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Junta de Castilla y León
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv AAA-ATPase
aba1
Chloroplast rupture
JAZ repressors
Cell death
Singlet oxygen
topic AAA-ATPase
aba1
Chloroplast rupture
JAZ repressors
Cell death
Singlet oxygen
description 44 páginas, 6 figuras, 1 tabla. -- The definitive version is available at http://www.elsevier.com
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/153694
url http://hdl.handle.net/10261/153694
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.jplph.2017.06.009
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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