Regulation of transcription elongation in response to osmostress

Cells trigger massive changes in gene expression upon environmental fluctuations. The Hog1 stress-activated protein kinase (SAPK) is an important regulator of the transcriptional activation program that maximizes cell fitness when yeast cells are exposed to osmostress. Besides being associated with...

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Autores: Silva, Andrea, Cavero, Santiago, Begley, Victoria, Solé, Carme, Böttcher, René, Chávez, Sebastián, Posas Garriga, Francesc, Nadal Clanchet, Eulàlia de
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/34223
Acceso en línea:http://hdl.handle.net/10230/34223
http://dx.doi.org/10.1371/journal.pgen.1007090
Access Level:acceso abierto
Palabra clave:Phosphorylation
Osmotic shock
Transcriptional control
Gene expression regulation
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spelling Regulation of transcription elongation in response to osmostressSilva, AndreaCavero, SantiagoBegley, VictoriaSolé, CarmeBöttcher, RenéChávez, SebastiánPosas Garriga, FrancescNadal Clanchet, Eulàlia dePhosphorylationOsmotic shockTranscriptional controlGene expression regulationCells trigger massive changes in gene expression upon environmental fluctuations. The Hog1 stress-activated protein kinase (SAPK) is an important regulator of the transcriptional activation program that maximizes cell fitness when yeast cells are exposed to osmostress. Besides being associated with transcription factors bound at target promoters to stimulate transcriptional initiation, activated Hog1 behaves as a transcriptional elongation factor that is selective for stress-responsive genes. Here, we provide insights into how this signaling kinase functions in transcription elongation. Hog1 phosphorylates the Spt4 elongation factor at Thr42 and Ser43 and such phosphorylations are essential for the overall transcriptional response upon osmostress. The phosphorylation of Spt4 by Hog1 regulates RNA polymerase II processivity at stress-responsive genes, which is critical for cell survival under high osmostress conditions. Thus, the direct regulation of Spt4 upon environmental insults serves to stimulate RNA Pol II elongation efficiency.AS was a recipient of an EMBO Long-Term Fellowship (EMBO ALTF 1394-2012). This work was supported by grants from the Spanish Ministry of Economy and Competitiveness (BFU2015-64437-P and FEDER, BFU2014-52125-REDT, BFU2014-51672-REDC to FP; BFU2014-52333-P and FEDER to EdN; BFU2016-77728-C3-1-P and BFU2013-48643-C3-1-P to SC), the Junta de Andalucía (P12-BIO1938MO to SC) and the Catalan Government (2014 SGR 599 to FP and EdN). This project was also supported by Fundación Botín, by Banco Santander through its Santander Universities Global Division to FP. FP is recipient of an ICREA Acadèmia (Generalitat de Catalunya).Public Library of Science (PLoS)201820182017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/34223http://dx.doi.org/10.1371/journal.pgen.1007090reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésPLOS Genetics. 2017 Nov 20;13(11):e1007090info:eu-repo/grantAgreement/ES/1PE/BFU2015-64437-Pinfo:eu-repo/grantAgreement/ES/1PE/BFU2014-52125-REDTinfo:eu-repo/grantAgreement/ES/1PE/BFU2014-51672-REDCinfo:eu-repo/grantAgreement/ES/1PE/BFU2014-52333-Pinfo:eu-repo/grantAgreement/ES/1PE/BFU2016-77728-C3-1-Pinfo:eu-repo/grantAgreement/ES/1PE/BFU2013-48643-C3-1-P© 2017 Silva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/342232026-05-29T05:05:01Z
dc.title.none.fl_str_mv Regulation of transcription elongation in response to osmostress
title Regulation of transcription elongation in response to osmostress
spellingShingle Regulation of transcription elongation in response to osmostress
Silva, Andrea
Phosphorylation
Osmotic shock
Transcriptional control
Gene expression regulation
title_short Regulation of transcription elongation in response to osmostress
title_full Regulation of transcription elongation in response to osmostress
title_fullStr Regulation of transcription elongation in response to osmostress
title_full_unstemmed Regulation of transcription elongation in response to osmostress
title_sort Regulation of transcription elongation in response to osmostress
dc.creator.none.fl_str_mv Silva, Andrea
Cavero, Santiago
Begley, Victoria
Solé, Carme
Böttcher, René
Chávez, Sebastián
Posas Garriga, Francesc
Nadal Clanchet, Eulàlia de
author Silva, Andrea
author_facet Silva, Andrea
Cavero, Santiago
Begley, Victoria
Solé, Carme
Böttcher, René
Chávez, Sebastián
Posas Garriga, Francesc
Nadal Clanchet, Eulàlia de
author_role author
author2 Cavero, Santiago
Begley, Victoria
Solé, Carme
Böttcher, René
Chávez, Sebastián
Posas Garriga, Francesc
Nadal Clanchet, Eulàlia de
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Phosphorylation
Osmotic shock
Transcriptional control
Gene expression regulation
topic Phosphorylation
Osmotic shock
Transcriptional control
Gene expression regulation
description Cells trigger massive changes in gene expression upon environmental fluctuations. The Hog1 stress-activated protein kinase (SAPK) is an important regulator of the transcriptional activation program that maximizes cell fitness when yeast cells are exposed to osmostress. Besides being associated with transcription factors bound at target promoters to stimulate transcriptional initiation, activated Hog1 behaves as a transcriptional elongation factor that is selective for stress-responsive genes. Here, we provide insights into how this signaling kinase functions in transcription elongation. Hog1 phosphorylates the Spt4 elongation factor at Thr42 and Ser43 and such phosphorylations are essential for the overall transcriptional response upon osmostress. The phosphorylation of Spt4 by Hog1 regulates RNA polymerase II processivity at stress-responsive genes, which is critical for cell survival under high osmostress conditions. Thus, the direct regulation of Spt4 upon environmental insults serves to stimulate RNA Pol II elongation efficiency.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/34223
http://dx.doi.org/10.1371/journal.pgen.1007090
url http://hdl.handle.net/10230/34223
http://dx.doi.org/10.1371/journal.pgen.1007090
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PLOS Genetics. 2017 Nov 20;13(11):e1007090
info:eu-repo/grantAgreement/ES/1PE/BFU2015-64437-P
info:eu-repo/grantAgreement/ES/1PE/BFU2014-52125-REDT
info:eu-repo/grantAgreement/ES/1PE/BFU2014-51672-REDC
info:eu-repo/grantAgreement/ES/1PE/BFU2014-52333-P
info:eu-repo/grantAgreement/ES/1PE/BFU2016-77728-C3-1-P
info:eu-repo/grantAgreement/ES/1PE/BFU2013-48643-C3-1-P
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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