Type I interferon response is delayed in human astrovirus infections

Type I interferon (IFN) activation and its subsequent effects are important in the response to viral infections. Here we show that human astroviruses (HAstVs), which are important agents of acute gastroenteritis in children, induce a mild and delayed IFN response upon infecting CaCo-2 cells. Althoug...

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Autores: Guix Arnau, Susana, Pérez Bosque, Anna, Miró, Luïsa, Moretó, Miquel, 1950-, Bosch, Albert, Pintó Solé, Rosa María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/64987
Acceso en línea:https://hdl.handle.net/2445/64987
Access Level:acceso abierto
Palabra clave:Malalties víriques
Interferó
Virus diseases
Interferon
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spelling Type I interferon response is delayed in human astrovirus infectionsGuix Arnau, SusanaPérez Bosque, AnnaMiró, LuïsaMoretó, Miquel, 1950-Bosch, AlbertPintó Solé, Rosa MaríaMalalties víriquesInterferóVirus diseasesInterferonType I interferon (IFN) activation and its subsequent effects are important in the response to viral infections. Here we show that human astroviruses (HAstVs), which are important agents of acute gastroenteritis in children, induce a mild and delayed IFN response upon infecting CaCo-2 cells. Although IFN-β mRNA is detected within infected cells and supernatant from infected cells show antiviral activity against the replication of other well-known IFN-sensitive viruses, these responses occur at late stages of infection once genome replication has taken place. On the other hand, HAstV replication can be partially reduced by the addition of exogenous IFN, and inhibition of IFN activation by BX795 enhances viral replication, indicating that HAstVs are IFN-sensitive viruses. Finally, different levels of IFN response were observed in cells infected with different HAstV mutants with changes in the hypervariable region of nsP1a/4, suggesting that nsP1a/4 genotype may potentially have clinical implications due to its correlation with the viral replication phenotype and the antiviral responses induced within infected cells.Public Library of Science (PLoS)2015201520152015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion18 p.application/pdfhttps://hdl.handle.net/2445/64987Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0123087PLoS One, 2015, vol. 10, num. , p. e0123087- e0123087http://dx.doi.org/10.1371/journal.pone.0123087cc-by (c) Guix Arnau, Susana et al., 2015http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/649872026-05-29T05:05:01Z
dc.title.none.fl_str_mv Type I interferon response is delayed in human astrovirus infections
title Type I interferon response is delayed in human astrovirus infections
spellingShingle Type I interferon response is delayed in human astrovirus infections
Guix Arnau, Susana
Malalties víriques
Interferó
Virus diseases
Interferon
title_short Type I interferon response is delayed in human astrovirus infections
title_full Type I interferon response is delayed in human astrovirus infections
title_fullStr Type I interferon response is delayed in human astrovirus infections
title_full_unstemmed Type I interferon response is delayed in human astrovirus infections
title_sort Type I interferon response is delayed in human astrovirus infections
dc.creator.none.fl_str_mv Guix Arnau, Susana
Pérez Bosque, Anna
Miró, Luïsa
Moretó, Miquel, 1950-
Bosch, Albert
Pintó Solé, Rosa María
author Guix Arnau, Susana
author_facet Guix Arnau, Susana
Pérez Bosque, Anna
Miró, Luïsa
Moretó, Miquel, 1950-
Bosch, Albert
Pintó Solé, Rosa María
author_role author
author2 Pérez Bosque, Anna
Miró, Luïsa
Moretó, Miquel, 1950-
Bosch, Albert
Pintó Solé, Rosa María
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Malalties víriques
Interferó
Virus diseases
Interferon
topic Malalties víriques
Interferó
Virus diseases
Interferon
description Type I interferon (IFN) activation and its subsequent effects are important in the response to viral infections. Here we show that human astroviruses (HAstVs), which are important agents of acute gastroenteritis in children, induce a mild and delayed IFN response upon infecting CaCo-2 cells. Although IFN-β mRNA is detected within infected cells and supernatant from infected cells show antiviral activity against the replication of other well-known IFN-sensitive viruses, these responses occur at late stages of infection once genome replication has taken place. On the other hand, HAstV replication can be partially reduced by the addition of exogenous IFN, and inhibition of IFN activation by BX795 enhances viral replication, indicating that HAstVs are IFN-sensitive viruses. Finally, different levels of IFN response were observed in cells infected with different HAstV mutants with changes in the hypervariable region of nsP1a/4, suggesting that nsP1a/4 genotype may potentially have clinical implications due to its correlation with the viral replication phenotype and the antiviral responses induced within infected cells.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/64987
url https://hdl.handle.net/2445/64987
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0123087
PLoS One, 2015, vol. 10, num. , p. e0123087- e0123087
http://dx.doi.org/10.1371/journal.pone.0123087
dc.rights.none.fl_str_mv cc-by (c) Guix Arnau, Susana et al., 2015
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Guix Arnau, Susana et al., 2015
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 18 p.
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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