Metabolic-inhibitor profiling links phenotype and transcriptome of Lachancea thermotolerans to wine fermentation chemistry

We applied targeted metabolic inhibitors to 145 Lachancea thermotolerans strains to uncover fermentation traits with direct relevance to wine quality. Oxamate, a lactate dehydrogenase inhibitor, reduced lactic acid and total titratable acidity by 21% and 26%, respectively, while increasing succinic...

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Detalles Bibliográficos
Autores: Jimena López, Samuel, Vicente Sánchez, Javier, Benito Sáez, Santiago, Marquina Díaz, Domingo, Santos de la Sen, Antonio
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/124626
Acceso en línea:https://hdl.handle.net/20.500.14352/124626
Access Level:acceso abierto
Palabra clave:579.67
577.15
Lachancea thermotolerans
Chemical composition
Metabolic inhibitors
Lactic fermentation
Lactic acid
Transcriptome
Microbiología (Biología)
Bioquímica (Biología)
Biotecnología
2414 Microbiología
3309.90 Microbiología de Alimentos
3309.92 Bioquímica y Microbiología de Los Procesos Fermentativos
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oai_identifier_str oai:docta.ucm.es:20.500.14352/124626
network_acronym_str ES
network_name_str España
repository_id_str
spelling Metabolic-inhibitor profiling links phenotype and transcriptome of Lachancea thermotolerans to wine fermentation chemistryJimena López, SamuelVicente Sánchez, JavierBenito Sáez, SantiagoMarquina Díaz, DomingoSantos de la Sen, Antonio579.67577.15Lachancea thermotoleransChemical compositionMetabolic inhibitorsLactic fermentationLactic acidTranscriptomeMicrobiología (Biología)Bioquímica (Biología)Biotecnología2414 Microbiología3309.90 Microbiología de Alimentos3309.92 Bioquímica y Microbiología de Los Procesos FermentativosWe applied targeted metabolic inhibitors to 145 Lachancea thermotolerans strains to uncover fermentation traits with direct relevance to wine quality. Oxamate, a lactate dehydrogenase inhibitor, reduced lactic acid and total titratable acidity by 21% and 26%, respectively, while increasing succinic acid and pH without affecting ethanol levels, offering a promising strategy to fine-tune wine freshness and balance. Notably, industrial grape-associated strains (clusters C4–C6) maintained robust growth under oxamate stress, unlike wild strains, positioning oxamate resistance as a practical marker for selecting high-performing, acidifying yeasts for winemaking. Additional inhibitors such as metformin shifted redox metabolism, significantly enhancing glycerol (+25%) and acetic acid (+319%) production. Transcriptomic analyses showed that OXA alone, and even more so the DSF + OXA combination, repressed LDH2 and upregulated GPD1 and oxidative phosphorylation genes, whereas MET caused only moderate changes. This integrated phenomic-transcriptomic approach not only provides valuable tools for yeast screening but also defines a roadmap for optimizing wine composition through the precision selection of L. thermotolerans strains.ElsevierUniversidad Complutense de Madrid20252025-09-0120252025-09-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/124626reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1246262026-06-02T12:44:21Z
dc.title.none.fl_str_mv Metabolic-inhibitor profiling links phenotype and transcriptome of Lachancea thermotolerans to wine fermentation chemistry
title Metabolic-inhibitor profiling links phenotype and transcriptome of Lachancea thermotolerans to wine fermentation chemistry
spellingShingle Metabolic-inhibitor profiling links phenotype and transcriptome of Lachancea thermotolerans to wine fermentation chemistry
Jimena López, Samuel
579.67
577.15
Lachancea thermotolerans
Chemical composition
Metabolic inhibitors
Lactic fermentation
Lactic acid
Transcriptome
Microbiología (Biología)
Bioquímica (Biología)
Biotecnología
2414 Microbiología
3309.90 Microbiología de Alimentos
3309.92 Bioquímica y Microbiología de Los Procesos Fermentativos
title_short Metabolic-inhibitor profiling links phenotype and transcriptome of Lachancea thermotolerans to wine fermentation chemistry
title_full Metabolic-inhibitor profiling links phenotype and transcriptome of Lachancea thermotolerans to wine fermentation chemistry
title_fullStr Metabolic-inhibitor profiling links phenotype and transcriptome of Lachancea thermotolerans to wine fermentation chemistry
title_full_unstemmed Metabolic-inhibitor profiling links phenotype and transcriptome of Lachancea thermotolerans to wine fermentation chemistry
title_sort Metabolic-inhibitor profiling links phenotype and transcriptome of Lachancea thermotolerans to wine fermentation chemistry
dc.creator.none.fl_str_mv Jimena López, Samuel
Vicente Sánchez, Javier
Benito Sáez, Santiago
Marquina Díaz, Domingo
Santos de la Sen, Antonio
author Jimena López, Samuel
author_facet Jimena López, Samuel
Vicente Sánchez, Javier
Benito Sáez, Santiago
Marquina Díaz, Domingo
Santos de la Sen, Antonio
author_role author
author2 Vicente Sánchez, Javier
Benito Sáez, Santiago
Marquina Díaz, Domingo
Santos de la Sen, Antonio
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 579.67
577.15
Lachancea thermotolerans
Chemical composition
Metabolic inhibitors
Lactic fermentation
Lactic acid
Transcriptome
Microbiología (Biología)
Bioquímica (Biología)
Biotecnología
2414 Microbiología
3309.90 Microbiología de Alimentos
3309.92 Bioquímica y Microbiología de Los Procesos Fermentativos
topic 579.67
577.15
Lachancea thermotolerans
Chemical composition
Metabolic inhibitors
Lactic fermentation
Lactic acid
Transcriptome
Microbiología (Biología)
Bioquímica (Biología)
Biotecnología
2414 Microbiología
3309.90 Microbiología de Alimentos
3309.92 Bioquímica y Microbiología de Los Procesos Fermentativos
description We applied targeted metabolic inhibitors to 145 Lachancea thermotolerans strains to uncover fermentation traits with direct relevance to wine quality. Oxamate, a lactate dehydrogenase inhibitor, reduced lactic acid and total titratable acidity by 21% and 26%, respectively, while increasing succinic acid and pH without affecting ethanol levels, offering a promising strategy to fine-tune wine freshness and balance. Notably, industrial grape-associated strains (clusters C4–C6) maintained robust growth under oxamate stress, unlike wild strains, positioning oxamate resistance as a practical marker for selecting high-performing, acidifying yeasts for winemaking. Additional inhibitors such as metformin shifted redox metabolism, significantly enhancing glycerol (+25%) and acetic acid (+319%) production. Transcriptomic analyses showed that OXA alone, and even more so the DSF + OXA combination, repressed LDH2 and upregulated GPD1 and oxidative phosphorylation genes, whereas MET caused only moderate changes. This integrated phenomic-transcriptomic approach not only provides valuable tools for yeast screening but also defines a roadmap for optimizing wine composition through the precision selection of L. thermotolerans strains.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-09-01
2025
2025-09-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/124626
url https://hdl.handle.net/20.500.14352/124626
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869409683273416704
score 15,812429

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