Comparative proteomic study of Edwardsiella tarda strains with different degrees of virulence.

Edwardsiella tarda is an enteric opportunistic pathogen that causes a great loss in aquaculture. This species has been described as a phenotypical homogeneous group; in contrast, serological studies and molecular typing revealed a wide heterogeneity. In this work, a proteomic study of differential e...

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Detalles Bibliográficos
Autores: Buján, Noemí, Hernández Haro, Carolina, Monteoliva Díaz, Lucía, Gil , Concha, Magariños, Beatriz
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/24207
Acceso en línea:https://hdl.handle.net/20.500.14352/24207
Access Level:acceso abierto
Palabra clave:579
Edwardsiella tarda
DIGE
Comparative proteomics
Virulence
Fish disease
Microbiología (Farmacia)
3302.03 Microbiología Industrial
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repository_id_str
spelling Comparative proteomic study of Edwardsiella tarda strains with different degrees of virulence.Buján, NoemíHernández Haro, CarolinaMonteoliva Díaz, LucíaGil , ConchaMagariños, Beatriz579Edwardsiella tardaDIGEComparative proteomicsVirulenceFish diseaseMicrobiología (Farmacia)3302.03 Microbiología IndustrialEdwardsiella tarda is an enteric opportunistic pathogen that causes a great loss in aquaculture. This species has been described as a phenotypical homogeneous group; in contrast, serological studies and molecular typing revealed a wide heterogeneity. In this work, a proteomic study of differential expression of a virulent isolate from turbot cultured in the Norwest of Spain in comparison with an avirulent collection strain was performed in order to recognize proteins involved in virulence. One hundred and three proteins that presented different abundance were successfully identified and classified into 11 functional categories according to their biological processes: amino acid, carbohydrate and lipid metabolism, tricarboxylic cycle, stress response and protein fate, protein synthesis, biogenesis of cellular components, cell rescue defence and virulence, cell membrane and transport, signal transduction and purine and pyrimidine metabolism. Twenty three protein spots detected only in turbot isolate were identified. It was shown that the same proteins appeared in different spots in the two isolates. Mass spectra obtained by MALDITOF/TOF of some of these proteins and DNA sequencing explained the changes as a result of different amino acid sequences. Several proteins related with the virulence of E. tarda (FliC, ArnA or FeSODI) were only detected in the turbot European isolate.ElsevierUniversidad Complutense de Madrid20152015-09-0820152015-09-08journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/24207reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available AGL2012-31049 ESTUDIO GENOMICO Y PROTEOMICO DE EDWARDSIELLA TARDA, UN PATOGENO EN LOS CULTIVOS DE PECES PLANOSopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/242072026-06-02T12:44:21Z
dc.title.none.fl_str_mv Comparative proteomic study of Edwardsiella tarda strains with different degrees of virulence.
title Comparative proteomic study of Edwardsiella tarda strains with different degrees of virulence.
spellingShingle Comparative proteomic study of Edwardsiella tarda strains with different degrees of virulence.
Buján, Noemí
579
Edwardsiella tarda
DIGE
Comparative proteomics
Virulence
Fish disease
Microbiología (Farmacia)
3302.03 Microbiología Industrial
title_short Comparative proteomic study of Edwardsiella tarda strains with different degrees of virulence.
title_full Comparative proteomic study of Edwardsiella tarda strains with different degrees of virulence.
title_fullStr Comparative proteomic study of Edwardsiella tarda strains with different degrees of virulence.
title_full_unstemmed Comparative proteomic study of Edwardsiella tarda strains with different degrees of virulence.
title_sort Comparative proteomic study of Edwardsiella tarda strains with different degrees of virulence.
dc.creator.none.fl_str_mv Buján, Noemí
Hernández Haro, Carolina
Monteoliva Díaz, Lucía
Gil , Concha
Magariños, Beatriz
author Buján, Noemí
author_facet Buján, Noemí
Hernández Haro, Carolina
Monteoliva Díaz, Lucía
Gil , Concha
Magariños, Beatriz
author_role author
author2 Hernández Haro, Carolina
Monteoliva Díaz, Lucía
Gil , Concha
Magariños, Beatriz
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 579
Edwardsiella tarda
DIGE
Comparative proteomics
Virulence
Fish disease
Microbiología (Farmacia)
3302.03 Microbiología Industrial
topic 579
Edwardsiella tarda
DIGE
Comparative proteomics
Virulence
Fish disease
Microbiología (Farmacia)
3302.03 Microbiología Industrial
description Edwardsiella tarda is an enteric opportunistic pathogen that causes a great loss in aquaculture. This species has been described as a phenotypical homogeneous group; in contrast, serological studies and molecular typing revealed a wide heterogeneity. In this work, a proteomic study of differential expression of a virulent isolate from turbot cultured in the Norwest of Spain in comparison with an avirulent collection strain was performed in order to recognize proteins involved in virulence. One hundred and three proteins that presented different abundance were successfully identified and classified into 11 functional categories according to their biological processes: amino acid, carbohydrate and lipid metabolism, tricarboxylic cycle, stress response and protein fate, protein synthesis, biogenesis of cellular components, cell rescue defence and virulence, cell membrane and transport, signal transduction and purine and pyrimidine metabolism. Twenty three protein spots detected only in turbot isolate were identified. It was shown that the same proteins appeared in different spots in the two isolates. Mass spectra obtained by MALDITOF/TOF of some of these proteins and DNA sequencing explained the changes as a result of different amino acid sequences. Several proteins related with the virulence of E. tarda (FliC, ArnA or FeSODI) were only detected in the turbot European isolate.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-09-08
2015
2015-09-08
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/24207
url https://hdl.handle.net/20.500.14352/24207
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available AGL2012-31049 ESTUDIO GENOMICO Y PROTEOMICO DE EDWARDSIELLA TARDA, UN PATOGENO EN LOS CULTIVOS DE PECES PLANOS
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301629

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