Composite nanofibrous membranes of PLGA/Aloe vera containing lipid nanoparticles for wound dressing applications

Electrospun nanofibrous dressings present suitable characteristics to be used in wound healing, such as high porosity and high surface area-to-volume ratio. In this study, a wound dressing based on PLGA and Aloe vera containing lipid nanoparticles (NLCs) was developed. NLCs were added in order to ad...

Descripción completa

Detalles Bibliográficos
Autores: García Orúe, Itxaso, Gainza Luzea, Garazi, García García, Patricia, Gutiérrez, Francisco Borja, Aguirre, José Javier, Hernández Martín, Rosa María, Delgado, Araceli, Igartua Olaechea, Manuela
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/71719
Acceso en línea:http://hdl.handle.net/10810/71719
Access Level:acceso abierto
Palabra clave:electrospinning
wound healing
aloe vera
PLGA
lipid nanoparticles
db/db mice
id ES_64552e3da996e81eaca4e4580997e400
oai_identifier_str oai:addi.ehu.eus:10810/71719
network_acronym_str ES
network_name_str España
repository_id_str
spelling Composite nanofibrous membranes of PLGA/Aloe vera containing lipid nanoparticles for wound dressing applicationsGarcía Orúe, ItxasoGainza Luzea, GaraziGarcía García, PatriciaGutiérrez, Francisco BorjaAguirre, José JavierHernández Martín, Rosa MaríaDelgado, AraceliIgartua Olaechea, Manuelaelectrospinningwound healingaloe veraPLGAlipid nanoparticlesdb/db miceElectrospun nanofibrous dressings present suitable characteristics to be used in wound healing, such as high porosity and high surface area-to-volume ratio. In this study, a wound dressing based on PLGA and Aloe vera containing lipid nanoparticles (NLCs) was developed. NLCs were added in order to add a lipid component that could avoid the adhesion of the dressing to the wound and improve its handling. Membranes with and without NLCs were composed of uniform fibers of about 1 µm in diameter. Their porosity was above 80 % and their thickness was about 160 µm. Both dressings showed similar water vapour transmission rate 1100 g/m2day. The formulation containing NLCs presented a higher ultimate tensile strength (2.61 ± 0.46 MPa) and a higher water uptake. Both formulations were biocompatible in vitro. Furthermore, the cell adhesion assay demonstrated that both membranes had a low adherence profile, although it was lower with the dressing containing NLCs. Finally, their efficacy was evaluated in a full thickness wound healing assay conducted in db/db mice, where both enhanced healing similarly. Accordingly, the PLGA-AV-NLC membrane might be a promising strategy for the treatment of chronic wounds, since it improved handling in comparison to the formulation without NLCs.I. García-Orue thanks the Basque Government for the fellowship grant. The authors thank for technical and human support provided by SGIker of UPV/EHU and European funding (ERDF and ESF). This project has been funded by the Basque Government (ELKARTEK 2015, Nanoplatform, KK-2015/0000036 and Consolidated Groups, IT-428-10 and IT-528-10).Elsevier202520252018info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/71719reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://doi.org/10.1016/j.ijpharm.2018.12.010info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/© 2018 Elsevier under CC BY-NC-ND licenseoai:addi.ehu.eus:10810/717192026-06-18T09:23:17Z
dc.title.none.fl_str_mv Composite nanofibrous membranes of PLGA/Aloe vera containing lipid nanoparticles for wound dressing applications
title Composite nanofibrous membranes of PLGA/Aloe vera containing lipid nanoparticles for wound dressing applications
spellingShingle Composite nanofibrous membranes of PLGA/Aloe vera containing lipid nanoparticles for wound dressing applications
García Orúe, Itxaso
electrospinning
wound healing
aloe vera
PLGA
lipid nanoparticles
db/db mice
title_short Composite nanofibrous membranes of PLGA/Aloe vera containing lipid nanoparticles for wound dressing applications
title_full Composite nanofibrous membranes of PLGA/Aloe vera containing lipid nanoparticles for wound dressing applications
title_fullStr Composite nanofibrous membranes of PLGA/Aloe vera containing lipid nanoparticles for wound dressing applications
title_full_unstemmed Composite nanofibrous membranes of PLGA/Aloe vera containing lipid nanoparticles for wound dressing applications
title_sort Composite nanofibrous membranes of PLGA/Aloe vera containing lipid nanoparticles for wound dressing applications
dc.creator.none.fl_str_mv García Orúe, Itxaso
Gainza Luzea, Garazi
García García, Patricia
Gutiérrez, Francisco Borja
Aguirre, José Javier
Hernández Martín, Rosa María
Delgado, Araceli
Igartua Olaechea, Manuela
author García Orúe, Itxaso
author_facet García Orúe, Itxaso
Gainza Luzea, Garazi
García García, Patricia
Gutiérrez, Francisco Borja
Aguirre, José Javier
Hernández Martín, Rosa María
Delgado, Araceli
Igartua Olaechea, Manuela
author_role author
author2 Gainza Luzea, Garazi
García García, Patricia
Gutiérrez, Francisco Borja
Aguirre, José Javier
Hernández Martín, Rosa María
Delgado, Araceli
Igartua Olaechea, Manuela
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv electrospinning
wound healing
aloe vera
PLGA
lipid nanoparticles
db/db mice
topic electrospinning
wound healing
aloe vera
PLGA
lipid nanoparticles
db/db mice
description Electrospun nanofibrous dressings present suitable characteristics to be used in wound healing, such as high porosity and high surface area-to-volume ratio. In this study, a wound dressing based on PLGA and Aloe vera containing lipid nanoparticles (NLCs) was developed. NLCs were added in order to add a lipid component that could avoid the adhesion of the dressing to the wound and improve its handling. Membranes with and without NLCs were composed of uniform fibers of about 1 µm in diameter. Their porosity was above 80 % and their thickness was about 160 µm. Both dressings showed similar water vapour transmission rate 1100 g/m2day. The formulation containing NLCs presented a higher ultimate tensile strength (2.61 ± 0.46 MPa) and a higher water uptake. Both formulations were biocompatible in vitro. Furthermore, the cell adhesion assay demonstrated that both membranes had a low adherence profile, although it was lower with the dressing containing NLCs. Finally, their efficacy was evaluated in a full thickness wound healing assay conducted in db/db mice, where both enhanced healing similarly. Accordingly, the PLGA-AV-NLC membrane might be a promising strategy for the treatment of chronic wounds, since it improved handling in comparison to the formulation without NLCs.
publishDate 2018
dc.date.none.fl_str_mv 2018
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/71719
url http://hdl.handle.net/10810/71719
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.ijpharm.2018.12.010
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
© 2018 Elsevier under CC BY-NC-ND license
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
© 2018 Elsevier under CC BY-NC-ND license
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869409638569476096
score 15.81155

Similares en LA Referencia