PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice.

Immature neurons are maintained in cortical regions of the adult mammalian brain. In rodents, many of these immature neurons can be identified in the piriform cortex based on their high expression of early neuronal markers, such as doublecortin (DCX) and the polysialylated form of the neural cell ad...

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Authors: Coviello, Simona, Benedetti, Bruno, Jakubecova, Dominika, Belles, Maria, Klimczak, Patrycja, Gramuntell, Yaiza, Couillard-Despres, Sebastien, Nacher, Juan
Format: article
Status:Published version
Publication Date:2021
Country:España
Institution:INCLIVA
Repository:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
OAI Identifier:oai:incliva.fundanetsuite.com:p15727
Online Access:https://incliva.portalinvestigacion.com/publicaciones/15727
Access Level:Open access
Keyword:PSA-NCAM
doublecortin
neuronal maturation
neuronal precursors
olfactory cortex
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spelling PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice.Coviello, SimonaBenedetti, BrunoJakubecova, DominikaBelles, MariaKlimczak, PatrycjaGramuntell, YaizaCouillard-Despres, SebastienNacher, JuanPSA-NCAMdoublecortinneuronal maturationneuronal precursorsolfactory cortexImmature neurons are maintained in cortical regions of the adult mammalian brain. In rodents, many of these immature neurons can be identified in the piriform cortex based on their high expression of early neuronal markers, such as doublecortin (DCX) and the polysialylated form of the neural cell adhesion molecule (PSA-NCAM). This molecule plays critical roles in different neurodevelopmental events. Taking advantage of a DCX-CreERT2/Flox-EGFP reporter mice, we investigated the impact of targeted PSA enzymatic depletion in the piriform cortex on the fate of immature neurons. We report here that the removal of PSA accelerated the final development of immature neurons. This was revealed by a higher frequency of NeuN expression, an increase in the number of cells carrying an axon initial segment (AIS), and an increase in the number of dendrites and dendritic spines on the immature neurons. Taken together, our results demonstrated the crucial role of the PSA moiety in the protracted development of immature neurons residing outside of the neurogenic niches. More studies will be required to understand the intrinsic and extrinsic factors affecting PSA-NCAM expression to understand how the brain regulates the incorporation of these immature neurons to the established neuronal circuits of the adult brain.MDPI2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://incliva.portalinvestigacion.com/publicaciones/15727INTERNATIONAL JOURNAL OF MOLECULAR SCIENCESISSN: 16616596ISSNe: 14220067reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVAinstname:INCLIVAInglésinfo:eu-repo/semantics/openAccessoai:incliva.fundanetsuite.com:p157272026-06-07T16:35:31Z
dc.title.none.fl_str_mv PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice.
title PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice.
spellingShingle PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice.
Coviello, Simona
PSA-NCAM
doublecortin
neuronal maturation
neuronal precursors
olfactory cortex
title_short PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice.
title_full PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice.
title_fullStr PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice.
title_full_unstemmed PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice.
title_sort PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice.
dc.creator.none.fl_str_mv Coviello, Simona
Benedetti, Bruno
Jakubecova, Dominika
Belles, Maria
Klimczak, Patrycja
Gramuntell, Yaiza
Couillard-Despres, Sebastien
Nacher, Juan
author Coviello, Simona
author_facet Coviello, Simona
Benedetti, Bruno
Jakubecova, Dominika
Belles, Maria
Klimczak, Patrycja
Gramuntell, Yaiza
Couillard-Despres, Sebastien
Nacher, Juan
author_role author
author2 Benedetti, Bruno
Jakubecova, Dominika
Belles, Maria
Klimczak, Patrycja
Gramuntell, Yaiza
Couillard-Despres, Sebastien
Nacher, Juan
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PSA-NCAM
doublecortin
neuronal maturation
neuronal precursors
olfactory cortex
topic PSA-NCAM
doublecortin
neuronal maturation
neuronal precursors
olfactory cortex
description Immature neurons are maintained in cortical regions of the adult mammalian brain. In rodents, many of these immature neurons can be identified in the piriform cortex based on their high expression of early neuronal markers, such as doublecortin (DCX) and the polysialylated form of the neural cell adhesion molecule (PSA-NCAM). This molecule plays critical roles in different neurodevelopmental events. Taking advantage of a DCX-CreERT2/Flox-EGFP reporter mice, we investigated the impact of targeted PSA enzymatic depletion in the piriform cortex on the fate of immature neurons. We report here that the removal of PSA accelerated the final development of immature neurons. This was revealed by a higher frequency of NeuN expression, an increase in the number of cells carrying an axon initial segment (AIS), and an increase in the number of dendrites and dendritic spines on the immature neurons. Taken together, our results demonstrated the crucial role of the PSA moiety in the protracted development of immature neurons residing outside of the neurogenic niches. More studies will be required to understand the intrinsic and extrinsic factors affecting PSA-NCAM expression to understand how the brain regulates the incorporation of these immature neurons to the established neuronal circuits of the adult brain.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://incliva.portalinvestigacion.com/publicaciones/15727
url https://incliva.portalinvestigacion.com/publicaciones/15727
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN: 16616596
ISSNe: 14220067
reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
instname:INCLIVA
instname_str INCLIVA
reponame_str r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
collection r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869409628279799808
score 15,812429

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