Volatile Transference and Antimicrobial Activity of Cheeses Made with Ewes’ Milk Fortified with Essential Oils

During the last decades, essential oils (EOs) have been proven to be a natural alternativeto additives or pasteurization for the prevention of microbial spoilage in several food matrices.In this work, we tested the antimicrobial activity of EOs from Melissa ocinalis, Ocimum basilicum,and Thymus vulg...

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Detalles Bibliográficos
Autores: Licón, Carmen C., Moro Peña, Armando, Librán, Celia, Molina Casanova, Ana María, Zalacain Aramburu, Amaya, Berruga Fernández, María Isabel, Carmona Delgado, Manuel
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad de Castilla-La Mancha
Repositorio:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/40125
Acceso en línea:https://doi.org/10.3390/foods9010035
https://www.mdpi.com/2304-8158/9/1/35/htm
https://hdl.handle.net/10578/40125
Access Level:acceso abierto
Palabra clave:Antimicrobial
Cheese
Clostridium tyrobutyricum
Escherichia coli
Essential oils
Penicillium verrucosum
Descripción
Sumario:During the last decades, essential oils (EOs) have been proven to be a natural alternativeto additives or pasteurization for the prevention of microbial spoilage in several food matrices.In this work, we tested the antimicrobial activity of EOs from Melissa ocinalis, Ocimum basilicum,and Thymus vulgaris against three dierent microorganisms: Escherichia coli, Clostridium tyrobutyricum,and Penicillium verrucosum. Pressed ewes cheese made from milk fortified with EOs (250 mg/kg) wasused as a model. The carryover eect of each oil was studied by analyzing the volatile fraction of dairysamples along the cheese making process using headspace stir bar sorptive extraction coupled to gaschromatography/mass spectrometry. Results showed that the EOs contained in T. vulgaris eectively reduced the counts of C. tyrobutyricum and inhibited completely the growth of P. verrucosum withoutaecting the natural flora present in the cheese. By contrast, the inhibitory eect of M. ocinalisagainst lactic acid bacteria starter cultures rendered this oil unsuitable for this matrix.