Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets
Understanding the interactions of graphene oxide (GO)-based materials with biological systems is critical due to the potential applications of these materials. Here, we investigate the extent to which single- to few-layer GO sheets of different controlled lateral dimensions translocate from the nose...
| Autores: | , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/308754 |
| Acceso en línea: | http://hdl.handle.net/10261/308754 https://api.elsevier.com/content/abstract/scopus_id/85093958679 |
| Access Level: | acceso abierto |
| Palabra clave: | 2D materials Biodegradation Biodistribution Brain Graphene oxide Inhalation Nanomaterials Nose-to-brain translocation |
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Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide NanosheetsNewman, LeonRodrigues, Artur FilipeJasim, DhifafVacchi, Isabella AnnaMénard‐Moyon, CéciliaBianco, AlbertoBussy, CyrillKostarelos, Kostas2D materialsBiodegradationBiodistributionBrainGraphene oxideInhalationNanomaterialsNose-to-brain translocationUnderstanding the interactions of graphene oxide (GO)-based materials with biological systems is critical due to the potential applications of these materials. Here, we investigate the extent to which single- to few-layer GO sheets of different controlled lateral dimensions translocate from the nose to the brain following intranasal instillation. We explore tissue location and in vivo biodegradability of the translocated materials using various techniques. Mass spectrometry and confocal Raman analyses indicate that trace amounts of GO undergo nose-to-brain translocation in a size-dependent manner. The smallest GO-sheet size category (us-GO, 10-550 nm) gains the greatest access to the brain in terms of quantity and coverage. Confocal Raman mapping and immunofluorescence combinations show that in vivo, us-GO resides in association with microglia. Point-and-shoot Raman spectroscopy shows that trace quantities of us-GO are maintained over 1 month, but undergo biodegradation-related changes. This study adds to growing awareness regarding the fate of graphene-based materials in biological systems.This work was partially supported by the UKRI Engineering and Physical Sciences Research Council (UKRI EPSRC) NowNano Centre for Doctoral Training programs (EP/K016946/1 and EP/M010619/1), and the European Union (EU) 7th and 8th Framework Programmes for Research and Technological Development, Graphene Flagship project (FP7-ICT-2013-FET-F-604391 and H2020-FET-696656–Graphene Core 1). We gratefully acknowledge the Centre National de la Recherche Scientifique (CNRS), the International Center for Frontier Research in Chemistry (icFRC), and financial support from the Agence Nationale de la Recherche (ANR) through the LabEx project Chemistry of Complex Systems (ANR-10-LABX-0026_CSC).Peer reviewedCell PressUK Research and InnovationEuropean CommissionCentre National de la Recherche Scientifique (France)Centre International de Recherche aux Frontières de la Chimie (France)Agence Nationale de la Recherche (France)Newman, Leon [0000-0003-2099-2660]Rodrigues, Artur Filipe [0000-0002-4078-3455]Jasim, Dhifaf [0000-0002-6433-4478]Bussy, Cyrill [0000-0001-8870-443X]Kostarelos, Kostas [0000-0002-2224-6672]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/308754https://api.elsevier.com/content/abstract/scopus_id/85093958679reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/604391info:eu-repo/grantAgreement/EC/H2020/696656https://doi.org/10.1016/j.xcrp.2020.100176Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3087542026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets |
| title |
Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets |
| spellingShingle |
Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets Newman, Leon 2D materials Biodegradation Biodistribution Brain Graphene oxide Inhalation Nanomaterials Nose-to-brain translocation |
| title_short |
Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets |
| title_full |
Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets |
| title_fullStr |
Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets |
| title_full_unstemmed |
Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets |
| title_sort |
Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets |
| dc.creator.none.fl_str_mv |
Newman, Leon Rodrigues, Artur Filipe Jasim, Dhifaf Vacchi, Isabella Anna Ménard‐Moyon, Cécilia Bianco, Alberto Bussy, Cyrill Kostarelos, Kostas |
| author |
Newman, Leon |
| author_facet |
Newman, Leon Rodrigues, Artur Filipe Jasim, Dhifaf Vacchi, Isabella Anna Ménard‐Moyon, Cécilia Bianco, Alberto Bussy, Cyrill Kostarelos, Kostas |
| author_role |
author |
| author2 |
Rodrigues, Artur Filipe Jasim, Dhifaf Vacchi, Isabella Anna Ménard‐Moyon, Cécilia Bianco, Alberto Bussy, Cyrill Kostarelos, Kostas |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
UK Research and Innovation European Commission Centre National de la Recherche Scientifique (France) Centre International de Recherche aux Frontières de la Chimie (France) Agence Nationale de la Recherche (France) Newman, Leon [0000-0003-2099-2660] Rodrigues, Artur Filipe [0000-0002-4078-3455] Jasim, Dhifaf [0000-0002-6433-4478] Bussy, Cyrill [0000-0001-8870-443X] Kostarelos, Kostas [0000-0002-2224-6672] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
2D materials Biodegradation Biodistribution Brain Graphene oxide Inhalation Nanomaterials Nose-to-brain translocation |
| topic |
2D materials Biodegradation Biodistribution Brain Graphene oxide Inhalation Nanomaterials Nose-to-brain translocation |
| description |
Understanding the interactions of graphene oxide (GO)-based materials with biological systems is critical due to the potential applications of these materials. Here, we investigate the extent to which single- to few-layer GO sheets of different controlled lateral dimensions translocate from the nose to the brain following intranasal instillation. We explore tissue location and in vivo biodegradability of the translocated materials using various techniques. Mass spectrometry and confocal Raman analyses indicate that trace amounts of GO undergo nose-to-brain translocation in a size-dependent manner. The smallest GO-sheet size category (us-GO, 10-550 nm) gains the greatest access to the brain in terms of quantity and coverage. Confocal Raman mapping and immunofluorescence combinations show that in vivo, us-GO resides in association with microglia. Point-and-shoot Raman spectroscopy shows that trace quantities of us-GO are maintained over 1 month, but undergo biodegradation-related changes. This study adds to growing awareness regarding the fate of graphene-based materials in biological systems. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2023 2023 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/308754 https://api.elsevier.com/content/abstract/scopus_id/85093958679 |
| url |
http://hdl.handle.net/10261/308754 https://api.elsevier.com/content/abstract/scopus_id/85093958679 |
| dc.language.none.fl_str_mv |
Inglés |
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Inglés |
| dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/FP7/604391 info:eu-repo/grantAgreement/EC/H2020/696656 https://doi.org/10.1016/j.xcrp.2020.100176 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Cell Press |
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Cell Press |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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