Two novel cyanobacterial bioluminescent whole-cell bioreporters based on superoxide dismutases MnSod and FeSod to detect superoxide anion

This Accepted Manuscript is available for reuse under a CC BY-NC-ND 3.0 licence after the 24 month embargo period provided that all the terms of the licence are adhered to

Detalhes bibliográficos
Autores: Hurtado Gallego, Jara, Martín-Betancor, K., Rodea Palomares, Ismael, Leganés Nieto, Francisco, Rosal, R., Fernández Piñas, Francisca
Formato: artículo
Fecha de publicación:2018
País:España
Recursos:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/687344
Acesso em linha:http://hdl.handle.net/10486/687344
https://dx.doi.org/10.1016/j.chemosphere.2018.03.012
Access Level:acceso abierto
Palavra-chave:Bioluminescence
Cyanobacterial bioreporter
Fe-sod
Mn-sod
Superoxide anion
Superoxide dismutases
Biología y Biomedicina / Biología
Química
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spelling Two novel cyanobacterial bioluminescent whole-cell bioreporters based on superoxide dismutases MnSod and FeSod to detect superoxide anionHurtado Gallego, JaraMartín-Betancor, K.Rodea Palomares, IsmaelLeganés Nieto, FranciscoRosal, R.Fernández Piñas, FranciscaBioluminescenceCyanobacterial bioreporterFe-sodMn-sodSuperoxide anionSuperoxide dismutasesBiología y Biomedicina / BiologíaQuímicaThis Accepted Manuscript is available for reuse under a CC BY-NC-ND 3.0 licence after the 24 month embargo period provided that all the terms of the licence are adhered toLtd This work describes the construction of two novel self-luminescent bioreporter strains of the cyanobacterium Nostoc sp. PCC 7120 by fusing the promoter region of the sodA and sodB genes (encoding the superoxide dismutases MnSod and FeSod, respectively) to luxCDABE from Photorhabdus luminescens aimed at detecting pollutants that generate reactive oxygen species (ROS), particularly O2−. Bioreporters were tested against methyl viologen (MV) as the inducer of superoxide anion (O2−). Both bioreporters were specific for O2− and Limits of detection (LODs) and Maximum Permissive Concentrations (MPCs) were calculated: Nostoc sp. PCC 7120 pBG2154 (sodA) had a range of detection from 400 to 1000 pM of MV and for Nostoc sp. PCC 7120 pBG2165 (sodB) the range of detection was from 500 to 1800 pM of MV after 5 h-exposure. To further validate the bioreporters, they were tested with the emerging pollutant Triclosan which induced bioluminescence in both strains. Furthermore, the bioreporters performance was tested in two real environmental samples with different water matrix complexity, spiked with MV. Both bioreporters were induced by O2− in these environmental samples. In the case of the river water sample, the amount of bioavailable MV as calculated from the bioreporters output was similar to that nominally added. For the waste water sample, the bioavailable MV concentration detected by the bioreporters was one order of magnitude lower than nominal. These differences could be due to MV complexation with organic matter and/or co-occurring organic contaminants. These results confirm their high sensitivity to O2− and their suitability to detect oxidative stress-generating pollutants in fresh-watersThis research is supported by CTM2013-45775-C2-1-R and CTM2013-45775-C2-2-R grants for MINECO . JHG is working under FPI contract (MINECO-EU)ElsevierDepartamento de BiologíaFacultad de Ciencias20182018-03-03research articlehttp://purl.org/coar/resource_type/c_2df8fbb1AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/687344https://dx.doi.org/10.1016/j.chemosphere.2018.03.012reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6873442026-06-23T12:46:27Z
dc.title.none.fl_str_mv Two novel cyanobacterial bioluminescent whole-cell bioreporters based on superoxide dismutases MnSod and FeSod to detect superoxide anion
title Two novel cyanobacterial bioluminescent whole-cell bioreporters based on superoxide dismutases MnSod and FeSod to detect superoxide anion
spellingShingle Two novel cyanobacterial bioluminescent whole-cell bioreporters based on superoxide dismutases MnSod and FeSod to detect superoxide anion
Hurtado Gallego, Jara
Bioluminescence
Cyanobacterial bioreporter
Fe-sod
Mn-sod
Superoxide anion
Superoxide dismutases
Biología y Biomedicina / Biología
Química
title_short Two novel cyanobacterial bioluminescent whole-cell bioreporters based on superoxide dismutases MnSod and FeSod to detect superoxide anion
title_full Two novel cyanobacterial bioluminescent whole-cell bioreporters based on superoxide dismutases MnSod and FeSod to detect superoxide anion
title_fullStr Two novel cyanobacterial bioluminescent whole-cell bioreporters based on superoxide dismutases MnSod and FeSod to detect superoxide anion
title_full_unstemmed Two novel cyanobacterial bioluminescent whole-cell bioreporters based on superoxide dismutases MnSod and FeSod to detect superoxide anion
title_sort Two novel cyanobacterial bioluminescent whole-cell bioreporters based on superoxide dismutases MnSod and FeSod to detect superoxide anion
dc.creator.none.fl_str_mv Hurtado Gallego, Jara
Martín-Betancor, K.
Rodea Palomares, Ismael
Leganés Nieto, Francisco
Rosal, R.
Fernández Piñas, Francisca
author Hurtado Gallego, Jara
author_facet Hurtado Gallego, Jara
Martín-Betancor, K.
Rodea Palomares, Ismael
Leganés Nieto, Francisco
Rosal, R.
Fernández Piñas, Francisca
author_role author
author2 Martín-Betancor, K.
Rodea Palomares, Ismael
Leganés Nieto, Francisco
Rosal, R.
Fernández Piñas, Francisca
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Biología
Facultad de Ciencias
dc.subject.none.fl_str_mv Bioluminescence
Cyanobacterial bioreporter
Fe-sod
Mn-sod
Superoxide anion
Superoxide dismutases
Biología y Biomedicina / Biología
Química
topic Bioluminescence
Cyanobacterial bioreporter
Fe-sod
Mn-sod
Superoxide anion
Superoxide dismutases
Biología y Biomedicina / Biología
Química
description This Accepted Manuscript is available for reuse under a CC BY-NC-ND 3.0 licence after the 24 month embargo period provided that all the terms of the licence are adhered to
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-03-03
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/687344
https://dx.doi.org/10.1016/j.chemosphere.2018.03.012
url http://hdl.handle.net/10486/687344
https://dx.doi.org/10.1016/j.chemosphere.2018.03.012
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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