RPL30 regulation of splicing reveals distinct roles for Cbp80 in U1 and U2 snRNP cotranscriptional recruitment

This article is Open Access.

Detalles Bibliográficos
Autores: Bragulat, Mireia, Meyer, Markus, Macías, Sara, Camats, Maria, Labrador, Mireia, Vilardell, Josep
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/87341
Acceso en línea:http://hdl.handle.net/10261/87341
Access Level:acceso abierto
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spelling RPL30 regulation of splicing reveals distinct roles for Cbp80 in U1 and U2 snRNP cotranscriptional recruitmentBragulat, MireiaMeyer, MarkusMacías, SaraCamats, MariaLabrador, MireiaVilardell, JosepThis article is Open Access.Pre-mRNA splicing is catalyzed by the spliceosome, and its control is essential for correct gene expression. While splicing repressors typically interfere with transcript recognition by spliceosomal components, the yeast protein L30 blocks spliceosomal rearrangements required for the engagement of U2 snRNP (small ribonucleoprotein particle) to its own transcript RPL30. Using a mutation in the RPL30 binding site that disrupts this repression, we have taken a genetic approach to reveal that regulation of splicing is restored in this mutant by deletion of the cap-binding complex (CBC) component Cbp80. Indeed, our data indicate that Cbp80 plays distinct roles in the recognition of the intron by U1 and U2 snRNP. It promotes the initial 5′ splice site recognition by U1 and, independently, facilitates U2 recruitment, depending on sequences located in the vicinity of the 5′ splice site. These results reveal a novel function for CBC in splicing and imply that these molecular events can be the target of a splicing regulator.This research has been supported by the MEC (BFU grants), an EU-MC Contract (#510183), and Agaur, S.M. and j.V. has been supported by a Ramón y Cajal/IP3 contract (MEC).Peer ReviewedCold Spring Harbor Laboratory Press2013201320102013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/87341reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1261/rna.2366310info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/873412026-05-22T06:33:51Z
dc.title.none.fl_str_mv RPL30 regulation of splicing reveals distinct roles for Cbp80 in U1 and U2 snRNP cotranscriptional recruitment
title RPL30 regulation of splicing reveals distinct roles for Cbp80 in U1 and U2 snRNP cotranscriptional recruitment
spellingShingle RPL30 regulation of splicing reveals distinct roles for Cbp80 in U1 and U2 snRNP cotranscriptional recruitment
Bragulat, Mireia
title_short RPL30 regulation of splicing reveals distinct roles for Cbp80 in U1 and U2 snRNP cotranscriptional recruitment
title_full RPL30 regulation of splicing reveals distinct roles for Cbp80 in U1 and U2 snRNP cotranscriptional recruitment
title_fullStr RPL30 regulation of splicing reveals distinct roles for Cbp80 in U1 and U2 snRNP cotranscriptional recruitment
title_full_unstemmed RPL30 regulation of splicing reveals distinct roles for Cbp80 in U1 and U2 snRNP cotranscriptional recruitment
title_sort RPL30 regulation of splicing reveals distinct roles for Cbp80 in U1 and U2 snRNP cotranscriptional recruitment
dc.creator.none.fl_str_mv Bragulat, Mireia
Meyer, Markus
Macías, Sara
Camats, Maria
Labrador, Mireia
Vilardell, Josep
author Bragulat, Mireia
author_facet Bragulat, Mireia
Meyer, Markus
Macías, Sara
Camats, Maria
Labrador, Mireia
Vilardell, Josep
author_role author
author2 Meyer, Markus
Macías, Sara
Camats, Maria
Labrador, Mireia
Vilardell, Josep
author2_role author
author
author
author
author
description This article is Open Access.
publishDate 2010
dc.date.none.fl_str_mv 2010
2013
2013
2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/87341
url http://hdl.handle.net/10261/87341
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1261/rna.2366310
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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