Physiological performance of transplastomic tobacco plants overexpressing aquaporin AQP1 in chloroplast membranes

The leaf mesophyll CO2 conductance and the concentration of CO2 within the chloroplast are major factors affecting photosynthetic performance. Previous studies have shown that the aquaporin NtAQP1 (which localizes to the plasma membrane and chloroplast inner envelope membrane) is involved in CO2 per...

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Authors: Fernández San Millán, Alicia, Aranjuelo Michelena, Iker, Ancín Rípodas, María, Larraya Reta, Luis María, Farrán Blanch, Inmaculada, Veramendi Charola, Jon
Format: article
Status:Published version
Publication Date:2018
Country:España
Institution:Universidad Pública de Navarra
Repository:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/38629
Online Access:https://hdl.handle.net/2454/38629
Access Level:Open access
Keyword:Aquaporin
Chloroplast envelope
CO2 permeability
Plastid transformation
Protein targeting
Tobacco
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spelling Physiological performance of transplastomic tobacco plants overexpressing aquaporin AQP1 in chloroplast membranesFernández San Millán, AliciaAranjuelo Michelena, IkerAncín Rípodas, MaríaLarraya Reta, Luis MaríaFarrán Blanch, InmaculadaVeramendi Charola, JonAquaporinChloroplast envelopeCO2 permeabilityPlastid transformationProtein targetingTobaccoThe leaf mesophyll CO2 conductance and the concentration of CO2 within the chloroplast are major factors affecting photosynthetic performance. Previous studies have shown that the aquaporin NtAQP1 (which localizes to the plasma membrane and chloroplast inner envelope membrane) is involved in CO2 permeability in the chloroplast. Levels of NtAQP1 in plants genetically engineered to overexpress the protein correlated positively with leaf mesophyll CO2 conductance and photosynthetic rate. In these studies, the nuclear transformation method used led to changes in NtAQP1 levels in the plasma membrane and the chloroplast inner envelope membrane. In the present work, NtAQP1 levels were increased up to 16-fold in the chloroplast membranes alone by the overexpression of NtAQP1 from the plastid genome. Despite the high NtAQP1 levels achieved, transplastomic plants showed lower photosynthetic rates than wild-type plants. This result was associated with lower Rubisco maximum carboxylation rate and ribulose 1,5-bisphosphate regeneration. Transplastomic plants showed reduced mesophyll CO2 conductance but no changes in chloroplast CO2 concentration. The absence of differences in chloroplast CO2 concentration was associated with the lower CO2 fixation activity of the transplastomic plants. These findings suggest that non-functional pores of recombinant NtAQP1 may be produced in the chloroplast inner envelope membrane.MA is a recipient of a Formación de Profesorado Universitario fellowship from the Ministerio de Educación, Cultura y Deporte, Spain. MN was supported by a predoctoral fellowship BES2015-072578 from the Ministerio de Economía y Competitividad (MINECO), Spain, co-financed by the European Science Foundation.Oxford University PressAgronomia, Bioteknologia eta ElikaduraAgronomía, Biotecnología y AlimentaciónIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/38629reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglés© The Author(s) 2018. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/386292026-06-17T12:41:47Z
dc.title.none.fl_str_mv Physiological performance of transplastomic tobacco plants overexpressing aquaporin AQP1 in chloroplast membranes
title Physiological performance of transplastomic tobacco plants overexpressing aquaporin AQP1 in chloroplast membranes
spellingShingle Physiological performance of transplastomic tobacco plants overexpressing aquaporin AQP1 in chloroplast membranes
Fernández San Millán, Alicia
Aquaporin
Chloroplast envelope
CO2 permeability
Plastid transformation
Protein targeting
Tobacco
title_short Physiological performance of transplastomic tobacco plants overexpressing aquaporin AQP1 in chloroplast membranes
title_full Physiological performance of transplastomic tobacco plants overexpressing aquaporin AQP1 in chloroplast membranes
title_fullStr Physiological performance of transplastomic tobacco plants overexpressing aquaporin AQP1 in chloroplast membranes
title_full_unstemmed Physiological performance of transplastomic tobacco plants overexpressing aquaporin AQP1 in chloroplast membranes
title_sort Physiological performance of transplastomic tobacco plants overexpressing aquaporin AQP1 in chloroplast membranes
dc.creator.none.fl_str_mv Fernández San Millán, Alicia
Aranjuelo Michelena, Iker
Ancín Rípodas, María
Larraya Reta, Luis María
Farrán Blanch, Inmaculada
Veramendi Charola, Jon
author Fernández San Millán, Alicia
author_facet Fernández San Millán, Alicia
Aranjuelo Michelena, Iker
Ancín Rípodas, María
Larraya Reta, Luis María
Farrán Blanch, Inmaculada
Veramendi Charola, Jon
author_role author
author2 Aranjuelo Michelena, Iker
Ancín Rípodas, María
Larraya Reta, Luis María
Farrán Blanch, Inmaculada
Veramendi Charola, Jon
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Agronomia, Bioteknologia eta Elikadura
Agronomía, Biotecnología y Alimentación
IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
dc.subject.none.fl_str_mv Aquaporin
Chloroplast envelope
CO2 permeability
Plastid transformation
Protein targeting
Tobacco
topic Aquaporin
Chloroplast envelope
CO2 permeability
Plastid transformation
Protein targeting
Tobacco
description The leaf mesophyll CO2 conductance and the concentration of CO2 within the chloroplast are major factors affecting photosynthetic performance. Previous studies have shown that the aquaporin NtAQP1 (which localizes to the plasma membrane and chloroplast inner envelope membrane) is involved in CO2 permeability in the chloroplast. Levels of NtAQP1 in plants genetically engineered to overexpress the protein correlated positively with leaf mesophyll CO2 conductance and photosynthetic rate. In these studies, the nuclear transformation method used led to changes in NtAQP1 levels in the plasma membrane and the chloroplast inner envelope membrane. In the present work, NtAQP1 levels were increased up to 16-fold in the chloroplast membranes alone by the overexpression of NtAQP1 from the plastid genome. Despite the high NtAQP1 levels achieved, transplastomic plants showed lower photosynthetic rates than wild-type plants. This result was associated with lower Rubisco maximum carboxylation rate and ribulose 1,5-bisphosphate regeneration. Transplastomic plants showed reduced mesophyll CO2 conductance but no changes in chloroplast CO2 concentration. The absence of differences in chloroplast CO2 concentration was associated with the lower CO2 fixation activity of the transplastomic plants. These findings suggest that non-functional pores of recombinant NtAQP1 may be produced in the chloroplast inner envelope membrane.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/38629
url https://hdl.handle.net/2454/38629
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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