Infusion of Phagocytic Macrophages Overexpressing CPT1a Ameliorates Kidney Fibrosis in the UUO Model

Phagocytosis is an inherent function of tissue macrophages for the removal of apoptotic cells and cellular debris during acute and chronic injury; however, the dynamics of this event during fibrosis development is unknown. We aim to prove that during the development of kidney fibrosis in the unilate...

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Autores: Calle, Priscila, Játiva, Soraya, Torrico, Selene, Muñoz, Angeles, García, Miriam, Sola Martínez, Anna, Serra i Cucurull, Dolors, Mera Nanín, Paula, Herrero Rodríguez, Laura, Hotter Corripio, Georgina
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/179750
Acesso em linha:https://hdl.handle.net/2445/179750
Access Level:acceso abierto
Palavra-chave:Malalties del ronyó
Fagocitosi
Macròfags
Kidney diseases
Phagocytosis
Macrophages
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spelling Infusion of Phagocytic Macrophages Overexpressing CPT1a Ameliorates Kidney Fibrosis in the UUO ModelCalle, PriscilaJátiva, SorayaTorrico, SeleneMuñoz, AngelesGarcía, MiriamSola Martínez, AnnaSerra i Cucurull, DolorsMera Nanín, PaulaHerrero Rodríguez, LauraHotter Corripio, GeorginaMalalties del ronyóFagocitosiMacròfagsKidney diseasesPhagocytosisMacrophagesPhagocytosis is an inherent function of tissue macrophages for the removal of apoptotic cells and cellular debris during acute and chronic injury; however, the dynamics of this event during fibrosis development is unknown. We aim to prove that during the development of kidney fibrosis in the unilateral ureteral obstruction (UUO) model, there are some populations of macrophage with a reduced ability to phagocytose, and whether the infusion of a population of phagocytic macrophages could reduce fibrosis in the murine model UUO. For this purpose, we have identified the macrophage populations during the development of fibrosis and have characterized their phagocytic ability and their expression of CPT1a. Furthermore, we have evaluated the therapeutic effect of macrophages overexpressing CPT1a with high phagocytic skills. We evidenced that the macrophage population which exhibits high phagocytic ability (F4/80low-CD11b) in fibrotic animals decreases during the progression of fibrosis while the macrophage population with lower phagocytic ability (F4/80high-CD11b) in fibrotic conditions, conversely, increases and CPT1a macrophage cell therapy with a strengthening phagocytic ability is associated with a therapeutic effect on kidney fibrosis. We have developed a therapeutic approach to reduce fibrosis in the UUO model by enrichment of the kidney resident macrophage population with a higher proportion of exogenous phagocytic macrophages overexpressing CPT1a.MDPI AG2021202120212021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion15 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/179750Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/cells10071650Cells, 2021, vol. 10, num. 7, p. 1650https://doi.org/10.3390/cells10071650cc by (c) Calle, Priscila et al., 2021http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1797502026-05-29T05:05:01Z
dc.title.none.fl_str_mv Infusion of Phagocytic Macrophages Overexpressing CPT1a Ameliorates Kidney Fibrosis in the UUO Model
title Infusion of Phagocytic Macrophages Overexpressing CPT1a Ameliorates Kidney Fibrosis in the UUO Model
spellingShingle Infusion of Phagocytic Macrophages Overexpressing CPT1a Ameliorates Kidney Fibrosis in the UUO Model
Calle, Priscila
Malalties del ronyó
Fagocitosi
Macròfags
Kidney diseases
Phagocytosis
Macrophages
title_short Infusion of Phagocytic Macrophages Overexpressing CPT1a Ameliorates Kidney Fibrosis in the UUO Model
title_full Infusion of Phagocytic Macrophages Overexpressing CPT1a Ameliorates Kidney Fibrosis in the UUO Model
title_fullStr Infusion of Phagocytic Macrophages Overexpressing CPT1a Ameliorates Kidney Fibrosis in the UUO Model
title_full_unstemmed Infusion of Phagocytic Macrophages Overexpressing CPT1a Ameliorates Kidney Fibrosis in the UUO Model
title_sort Infusion of Phagocytic Macrophages Overexpressing CPT1a Ameliorates Kidney Fibrosis in the UUO Model
dc.creator.none.fl_str_mv Calle, Priscila
Játiva, Soraya
Torrico, Selene
Muñoz, Angeles
García, Miriam
Sola Martínez, Anna
Serra i Cucurull, Dolors
Mera Nanín, Paula
Herrero Rodríguez, Laura
Hotter Corripio, Georgina
author Calle, Priscila
author_facet Calle, Priscila
Játiva, Soraya
Torrico, Selene
Muñoz, Angeles
García, Miriam
Sola Martínez, Anna
Serra i Cucurull, Dolors
Mera Nanín, Paula
Herrero Rodríguez, Laura
Hotter Corripio, Georgina
author_role author
author2 Játiva, Soraya
Torrico, Selene
Muñoz, Angeles
García, Miriam
Sola Martínez, Anna
Serra i Cucurull, Dolors
Mera Nanín, Paula
Herrero Rodríguez, Laura
Hotter Corripio, Georgina
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Malalties del ronyó
Fagocitosi
Macròfags
Kidney diseases
Phagocytosis
Macrophages
topic Malalties del ronyó
Fagocitosi
Macròfags
Kidney diseases
Phagocytosis
Macrophages
description Phagocytosis is an inherent function of tissue macrophages for the removal of apoptotic cells and cellular debris during acute and chronic injury; however, the dynamics of this event during fibrosis development is unknown. We aim to prove that during the development of kidney fibrosis in the unilateral ureteral obstruction (UUO) model, there are some populations of macrophage with a reduced ability to phagocytose, and whether the infusion of a population of phagocytic macrophages could reduce fibrosis in the murine model UUO. For this purpose, we have identified the macrophage populations during the development of fibrosis and have characterized their phagocytic ability and their expression of CPT1a. Furthermore, we have evaluated the therapeutic effect of macrophages overexpressing CPT1a with high phagocytic skills. We evidenced that the macrophage population which exhibits high phagocytic ability (F4/80low-CD11b) in fibrotic animals decreases during the progression of fibrosis while the macrophage population with lower phagocytic ability (F4/80high-CD11b) in fibrotic conditions, conversely, increases and CPT1a macrophage cell therapy with a strengthening phagocytic ability is associated with a therapeutic effect on kidney fibrosis. We have developed a therapeutic approach to reduce fibrosis in the UUO model by enrichment of the kidney resident macrophage population with a higher proportion of exogenous phagocytic macrophages overexpressing CPT1a.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/179750
url https://hdl.handle.net/2445/179750
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/cells10071650
Cells, 2021, vol. 10, num. 7, p. 1650
https://doi.org/10.3390/cells10071650
dc.rights.none.fl_str_mv cc by (c) Calle, Priscila et al., 2021
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Calle, Priscila et al., 2021
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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