Evaluation of the allergenicity of goat milk, cow milk, and their lactosera in a guinea pig model

The object of this study was to determine the allergenicity of goat milk (GM) and cow milk (CM) and that of their respective lactosera (GML and CML), by in vivo and in vitro assays. Two systemic tests for anaphylaxis were carried out in guinea pigs, the animals being sensitized orally with the 2 typ...

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Autores: Sanz Ceballos, Laura, Sanz-Sampelayo, María Remedios, Gil Extremera, Francisca, Rodríguez Osorio, Matilde
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/275540
Acceso en línea:http://hdl.handle.net/10261/275540
Access Level:acceso abierto
Palabra clave:Allergenicity
Serology
Goat and cow milk
Goat and cow milk lactosera
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spelling Evaluation of the allergenicity of goat milk, cow milk, and their lactosera in a guinea pig modelSanz Ceballos, LauraSanz-Sampelayo, María RemediosGil Extremera, FranciscaRodríguez Osorio, MatildeAllergenicitySerologyGoat and cow milkGoat and cow milk lactoseraThe object of this study was to determine the allergenicity of goat milk (GM) and cow milk (CM) and that of their respective lactosera (GML and CML), by in vivo and in vitro assays. Two systemic tests for anaphylaxis were carried out in guinea pigs, the animals being sensitized orally with the 2 types of milk and lactosera. Sera were taken from the orbital sinus of the experimental animals at 0 and 22 d of the experiment to perform the serological study and the passive cutaneous anaphylaxis test. For the latter, the guinea pigs were sensitized passively with antibodies against the 4 antigen solutions. Enzyme-linked immunosorbent assay and Western blot were used to determine the specific antibodies of the isotypes immunoglobulin G1 and immunoglobulin G(Fc) developed against the same 4 antigen solutions. From these anaphylaxis and antibody-production tests, it was concluded that GM is hypoallergenic when compared with CM. The lactosera produced more closely grouped results, with values always below those of the corresponding milk. None of the proteins in the 4 immunizing solutions were identified as being their main allergen. These results show the hypoallergenicity of GM versus CM, and also that both casein and lactoserum proteins may be responsible for allergy in each case. To analyze the possibility of producing an innocuous food for those allergic to milk proteins, it would be of interest to identify the epitope(s) responsible for such allergenicity.This study was supported financially by the Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía, Spain (project: C03-045).Peer reviewedElsevierJunta de AndalucíaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222009info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/275540reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3168/jds.2008-1125Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2755402026-05-22T06:33:51Z
dc.title.none.fl_str_mv Evaluation of the allergenicity of goat milk, cow milk, and their lactosera in a guinea pig model
title Evaluation of the allergenicity of goat milk, cow milk, and their lactosera in a guinea pig model
spellingShingle Evaluation of the allergenicity of goat milk, cow milk, and their lactosera in a guinea pig model
Sanz Ceballos, Laura
Allergenicity
Serology
Goat and cow milk
Goat and cow milk lactosera
title_short Evaluation of the allergenicity of goat milk, cow milk, and their lactosera in a guinea pig model
title_full Evaluation of the allergenicity of goat milk, cow milk, and their lactosera in a guinea pig model
title_fullStr Evaluation of the allergenicity of goat milk, cow milk, and their lactosera in a guinea pig model
title_full_unstemmed Evaluation of the allergenicity of goat milk, cow milk, and their lactosera in a guinea pig model
title_sort Evaluation of the allergenicity of goat milk, cow milk, and their lactosera in a guinea pig model
dc.creator.none.fl_str_mv Sanz Ceballos, Laura
Sanz-Sampelayo, María Remedios
Gil Extremera, Francisca
Rodríguez Osorio, Matilde
author Sanz Ceballos, Laura
author_facet Sanz Ceballos, Laura
Sanz-Sampelayo, María Remedios
Gil Extremera, Francisca
Rodríguez Osorio, Matilde
author_role author
author2 Sanz-Sampelayo, María Remedios
Gil Extremera, Francisca
Rodríguez Osorio, Matilde
author2_role author
author
author
dc.contributor.none.fl_str_mv Junta de Andalucía
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Allergenicity
Serology
Goat and cow milk
Goat and cow milk lactosera
topic Allergenicity
Serology
Goat and cow milk
Goat and cow milk lactosera
description The object of this study was to determine the allergenicity of goat milk (GM) and cow milk (CM) and that of their respective lactosera (GML and CML), by in vivo and in vitro assays. Two systemic tests for anaphylaxis were carried out in guinea pigs, the animals being sensitized orally with the 2 types of milk and lactosera. Sera were taken from the orbital sinus of the experimental animals at 0 and 22 d of the experiment to perform the serological study and the passive cutaneous anaphylaxis test. For the latter, the guinea pigs were sensitized passively with antibodies against the 4 antigen solutions. Enzyme-linked immunosorbent assay and Western blot were used to determine the specific antibodies of the isotypes immunoglobulin G1 and immunoglobulin G(Fc) developed against the same 4 antigen solutions. From these anaphylaxis and antibody-production tests, it was concluded that GM is hypoallergenic when compared with CM. The lactosera produced more closely grouped results, with values always below those of the corresponding milk. None of the proteins in the 4 immunizing solutions were identified as being their main allergen. These results show the hypoallergenicity of GM versus CM, and also that both casein and lactoserum proteins may be responsible for allergy in each case. To analyze the possibility of producing an innocuous food for those allergic to milk proteins, it would be of interest to identify the epitope(s) responsible for such allergenicity.
publishDate 2009
dc.date.none.fl_str_mv 2009
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/275540
url http://hdl.handle.net/10261/275540
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3168/jds.2008-1125
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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