Exploring current tendencies in techniques and materials for immobilization of laccases – A review

Nanotechnology has transformed the science behind many biotechnological sectors, and applied bio-catalysis is not the exception. In 2017, the enzyme industry was valued at more than 7 billion USD and projected to 10.5 billion by 2024. The laccase enzyme is an oxidoreductase capable of oxidizing phen...

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Detalles Bibliográficos
Autores: Alvarado-Ramírez, Lynette, Rostro-Alanis, Magdalena, Rodríguez-Rodríguez, José, Castillo-Zacarías, Carlos, Sosa-Hernández, Juan Eduardo, Barceló, Damià, Iqbal, Hafiz M.N., Parra-Saldívar, Roberto
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/240382
Acceso en línea:http://hdl.handle.net/10261/240382
Access Level:acceso abierto
Palabra clave:Immobilization
Laccases
Bio-catalysis
Nanocarriers
Novel techniques
Biological macromolecules
Descripción
Sumario:Nanotechnology has transformed the science behind many biotechnological sectors, and applied bio-catalysis is not the exception. In 2017, the enzyme industry was valued at more than 7 billion USD and projected to 10.5 billion by 2024. The laccase enzyme is an oxidoreductase capable of oxidizing phenolic and non-phenolic compounds that have been considered an essential tool in the fields currently known as white biotechnology and green chemistry. Laccase is one of the most robust biocatalysts due to its wide applications in different environmental processes such as detecting and treating chemical pollutants and dyes and pharmaceutical removal. However, these biocatalytic processes are usually limited by the lack of stability of the enzyme, the half-life time, and the application feasibility at an industrial scale. Physical or chemical approaches have performed different laccase's immobilization methods to improve its catalytic properties and reuse. Emerging technologies have been proven to reduce the manufacturing process cost and increase application feasibility while looking for ecological and economical materials that can be used as support. Therefore, this review discusses the trends of enzyme immobilization recently studied, analyzing biomaterials and agro-industrial waste used for that intention, their advantages, and disadvantages. Finally, the work also highlights the performance obtained with these materials and current challenges and potential alternatives.