The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last steps

The 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway provides the precursors for the biosynthesis of plastidial isoprenoids, which include the carotenoid pigments of many fruits. We have analysed the genes encoding the seven enzymes of the MEP pathway in melon (Cucumis melo L.) and determined that...

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Autores: Saladié, Montserrat|||0000-0002-0088-7233, Wright, Louwrance P., Garcia-Mas, Jordi|||0000-0001-7101-9049, Rodríguez Concepción, Manuel|||0000-0002-1280-2305, Phillips, Michael A.
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:217725
Acceso en línea:https://ddd.uab.cat/record/217725
https://dx.doi.org/urn:doi:10.1093/jxb/eru275
Access Level:acceso abierto
Palabra clave:2-C-methylerythritol 4-phosphate
Carotenoids
Fruit development
Isoprenoid biosynthesis
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spelling The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last stepsSaladié, Montserrat|||0000-0002-0088-7233Wright, Louwrance P.Garcia-Mas, Jordi|||0000-0001-7101-9049Rodríguez Concepción, Manuel|||0000-0002-1280-2305Phillips, Michael A.2-C-methylerythritol 4-phosphateCarotenoidsFruit developmentIsoprenoid biosynthesisThe 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway provides the precursors for the biosynthesis of plastidial isoprenoids, which include the carotenoid pigments of many fruits. We have analysed the genes encoding the seven enzymes of the MEP pathway in melon (Cucumis melo L.) and determined that the first one, 1-deoxyxylulose 5-phosphate synthase (DXS), and the last one, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (HDR), are represented in the genome as a small gene family and paralogous pair, respectively. In the case of DXS, three genes encode functional DXS activities which fall into previously established type I (CmDXS1) and II (CmDXS2a and CmDXS2b) categories, while a fourth DXS-like gene belonging to the type III group did not encode a protein with DXS activity. Their expression patterns and phylogenies suggest that CmDXS1 is functionally specialized for developmental and photosynthetic processes, while CmDXS2a and CmDXS2b are induced in flowers and ripening fruit of orange- (but not white-) fleshed varieties, coinciding with β-carotene accumulation. This is the first instance connecting type II DXS genes to specialized isoprenoid biosynthesis in the fruit of an agronomically important species. Two HDR paralogues were shown to encode functional enzymes, although only CmHDR1 was highly expressed in the tissues and developmental stages tested. Phylogenetic analysis showed that in cucurbits such as melon, these HDR paralogues probably arose through individual gene duplications in a common angiosperm ancestor, mimicking a prior division in gymnosperms, while other flowering plants, including apple, soy, canola, and poplar, acquired HDR duplicates recently as homoeologues through large-scale genome duplications. We report the influence of gene duplication history on the regulation of the MEP pathway in melon and the role of specialized MEP-pathway isoforms in providing precursors for β-carotene production in orange-fleshed melon varieties. 22014-01-0120142014-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/217725https://dx.doi.org/urn:doi:10.1093/jxb/eru275reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 BIO2011-23680Ministerio de Educación y Ciencia https://doi.org/10.13039/501100004837 CSD2007-00036Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 GEN2006-27773-C2-1open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2177252026-06-06T12:50:31Z
dc.title.none.fl_str_mv The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last steps
title The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last steps
spellingShingle The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last steps
Saladié, Montserrat|||0000-0002-0088-7233
2-C-methylerythritol 4-phosphate
Carotenoids
Fruit development
Isoprenoid biosynthesis
title_short The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last steps
title_full The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last steps
title_fullStr The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last steps
title_full_unstemmed The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last steps
title_sort The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last steps
dc.creator.none.fl_str_mv Saladié, Montserrat|||0000-0002-0088-7233
Wright, Louwrance P.
Garcia-Mas, Jordi|||0000-0001-7101-9049
Rodríguez Concepción, Manuel|||0000-0002-1280-2305
Phillips, Michael A.
author Saladié, Montserrat|||0000-0002-0088-7233
author_facet Saladié, Montserrat|||0000-0002-0088-7233
Wright, Louwrance P.
Garcia-Mas, Jordi|||0000-0001-7101-9049
Rodríguez Concepción, Manuel|||0000-0002-1280-2305
Phillips, Michael A.
author_role author
author2 Wright, Louwrance P.
Garcia-Mas, Jordi|||0000-0001-7101-9049
Rodríguez Concepción, Manuel|||0000-0002-1280-2305
Phillips, Michael A.
author2_role author
author
author
author
dc.subject.none.fl_str_mv 2-C-methylerythritol 4-phosphate
Carotenoids
Fruit development
Isoprenoid biosynthesis
topic 2-C-methylerythritol 4-phosphate
Carotenoids
Fruit development
Isoprenoid biosynthesis
description The 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway provides the precursors for the biosynthesis of plastidial isoprenoids, which include the carotenoid pigments of many fruits. We have analysed the genes encoding the seven enzymes of the MEP pathway in melon (Cucumis melo L.) and determined that the first one, 1-deoxyxylulose 5-phosphate synthase (DXS), and the last one, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (HDR), are represented in the genome as a small gene family and paralogous pair, respectively. In the case of DXS, three genes encode functional DXS activities which fall into previously established type I (CmDXS1) and II (CmDXS2a and CmDXS2b) categories, while a fourth DXS-like gene belonging to the type III group did not encode a protein with DXS activity. Their expression patterns and phylogenies suggest that CmDXS1 is functionally specialized for developmental and photosynthetic processes, while CmDXS2a and CmDXS2b are induced in flowers and ripening fruit of orange- (but not white-) fleshed varieties, coinciding with β-carotene accumulation. This is the first instance connecting type II DXS genes to specialized isoprenoid biosynthesis in the fruit of an agronomically important species. Two HDR paralogues were shown to encode functional enzymes, although only CmHDR1 was highly expressed in the tissues and developmental stages tested. Phylogenetic analysis showed that in cucurbits such as melon, these HDR paralogues probably arose through individual gene duplications in a common angiosperm ancestor, mimicking a prior division in gymnosperms, while other flowering plants, including apple, soy, canola, and poplar, acquired HDR duplicates recently as homoeologues through large-scale genome duplications. We report the influence of gene duplication history on the regulation of the MEP pathway in melon and the role of specialized MEP-pathway isoforms in providing precursors for β-carotene production in orange-fleshed melon varieties.
publishDate 2014
dc.date.none.fl_str_mv 2
2014-01-01
2014
2014-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/217725
https://dx.doi.org/urn:doi:10.1093/jxb/eru275
url https://ddd.uab.cat/record/217725
https://dx.doi.org/urn:doi:10.1093/jxb/eru275
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 BIO2011-23680
Ministerio de Educación y Ciencia https://doi.org/10.13039/501100004837 CSD2007-00036
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 GEN2006-27773-C2-1
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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repository.mail.fl_str_mv
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