Strain-specific interspecies interactions between co-isolated pairs of Staphylococcus aureus and Pseudomonas aeruginosa from patients with tracheobronchitis or bronchial colonization

Dual species interactions in co-isolated pairs of Staphylococcus aureus and Pseudomonas aeruginosa from patients with tracheobronchitis or bronchial colonization were examined. The genetic and phenotypic diversity between the isolates was high making the interactions detected strain-specific. Despit...

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Autores: Gomes Fernandes, Meissiner|||0000-0002-9454-8848, Gómez Camacho, Andromeda Celeste|||0000-0002-0075-6903, Bravo, Marc|||0000-0002-3173-2242, Huedo Moreno, Pol|||0000-0002-2933-0586, Coves, Xavier|||0000-0002-8970-8907, Prat i Aymerich, Cristina|||0000-0001-6974-9165, Gibert, Isidre|||0000-0003-1442-2258, Lacoma, Alicia|||0000-0002-2049-3872, Yero, Daniel|||0000-0002-6234-4082
Tipo de documento: artigo
Data de publicação:2022
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:ddd.uab.cat:257290
Acesso em linha:https://ddd.uab.cat/record/257290
https://dx.doi.org/urn:doi:10.1038/s41598-022-07018-5
Access Level:Acceso aberto
Palavra-chave:Bacterial pathogenesis
Biofilms
Pathogens
Clinical microbiology
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spelling Strain-specific interspecies interactions between co-isolated pairs of Staphylococcus aureus and Pseudomonas aeruginosa from patients with tracheobronchitis or bronchial colonizationGomes Fernandes, Meissiner|||0000-0002-9454-8848Gómez Camacho, Andromeda Celeste|||0000-0002-0075-6903Bravo, Marc|||0000-0002-3173-2242Huedo Moreno, Pol|||0000-0002-2933-0586Coves, Xavier|||0000-0002-8970-8907Prat i Aymerich, Cristina|||0000-0001-6974-9165Gibert, Isidre|||0000-0003-1442-2258Lacoma, Alicia|||0000-0002-2049-3872Yero, Daniel|||0000-0002-6234-4082Bacterial pathogenesisBiofilmsPathogensClinical microbiologyDual species interactions in co-isolated pairs of Staphylococcus aureus and Pseudomonas aeruginosa from patients with tracheobronchitis or bronchial colonization were examined. The genetic and phenotypic diversity between the isolates was high making the interactions detected strain-specific. Despite this, and the clinical origin of the strains, some interactions were common between some co-isolated pairs. For most pairs, P. aeruginosa exoproducts affected biofilm formation and reduced growth in vitro in its S. aureus counterpart. Conversely, S. aureus did not impair biofilm formation and stimulated swarming motility in P. aeruginosa. Co-culture in a medium that mimics respiratory mucus promoted coexistence and favored mixed microcolony formation within biofilms. Under these conditions, key genes controlled by quorum sensing were differentially regulated in both species in an isolate-dependent manner. Finally, co-infection in the acute infection model in Galleria mellonella larvae showed an additive effect only in the co-isolated pair in which P. aeruginosa affected less S. aureus growth. This work contributes to understanding the complex interspecies interactions between P. aeruginosa and S. aureus by studying strains isolated during acute infection. 22022-01-0120222022-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/257290https://dx.doi.org/urn:doi:10.1038/s41598-022-07018-5reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengInstituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI17/01139Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1062Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-111364RB-I00open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2572902026-06-06T12:50:31Z
dc.title.none.fl_str_mv Strain-specific interspecies interactions between co-isolated pairs of Staphylococcus aureus and Pseudomonas aeruginosa from patients with tracheobronchitis or bronchial colonization
title Strain-specific interspecies interactions between co-isolated pairs of Staphylococcus aureus and Pseudomonas aeruginosa from patients with tracheobronchitis or bronchial colonization
spellingShingle Strain-specific interspecies interactions between co-isolated pairs of Staphylococcus aureus and Pseudomonas aeruginosa from patients with tracheobronchitis or bronchial colonization
Gomes Fernandes, Meissiner|||0000-0002-9454-8848
Bacterial pathogenesis
Biofilms
Pathogens
Clinical microbiology
title_short Strain-specific interspecies interactions between co-isolated pairs of Staphylococcus aureus and Pseudomonas aeruginosa from patients with tracheobronchitis or bronchial colonization
title_full Strain-specific interspecies interactions between co-isolated pairs of Staphylococcus aureus and Pseudomonas aeruginosa from patients with tracheobronchitis or bronchial colonization
title_fullStr Strain-specific interspecies interactions between co-isolated pairs of Staphylococcus aureus and Pseudomonas aeruginosa from patients with tracheobronchitis or bronchial colonization
title_full_unstemmed Strain-specific interspecies interactions between co-isolated pairs of Staphylococcus aureus and Pseudomonas aeruginosa from patients with tracheobronchitis or bronchial colonization
title_sort Strain-specific interspecies interactions between co-isolated pairs of Staphylococcus aureus and Pseudomonas aeruginosa from patients with tracheobronchitis or bronchial colonization
dc.creator.none.fl_str_mv Gomes Fernandes, Meissiner|||0000-0002-9454-8848
Gómez Camacho, Andromeda Celeste|||0000-0002-0075-6903
Bravo, Marc|||0000-0002-3173-2242
Huedo Moreno, Pol|||0000-0002-2933-0586
Coves, Xavier|||0000-0002-8970-8907
Prat i Aymerich, Cristina|||0000-0001-6974-9165
Gibert, Isidre|||0000-0003-1442-2258
Lacoma, Alicia|||0000-0002-2049-3872
Yero, Daniel|||0000-0002-6234-4082
author Gomes Fernandes, Meissiner|||0000-0002-9454-8848
author_facet Gomes Fernandes, Meissiner|||0000-0002-9454-8848
Gómez Camacho, Andromeda Celeste|||0000-0002-0075-6903
Bravo, Marc|||0000-0002-3173-2242
Huedo Moreno, Pol|||0000-0002-2933-0586
Coves, Xavier|||0000-0002-8970-8907
Prat i Aymerich, Cristina|||0000-0001-6974-9165
Gibert, Isidre|||0000-0003-1442-2258
Lacoma, Alicia|||0000-0002-2049-3872
Yero, Daniel|||0000-0002-6234-4082
author_role author
author2 Gómez Camacho, Andromeda Celeste|||0000-0002-0075-6903
Bravo, Marc|||0000-0002-3173-2242
Huedo Moreno, Pol|||0000-0002-2933-0586
Coves, Xavier|||0000-0002-8970-8907
Prat i Aymerich, Cristina|||0000-0001-6974-9165
Gibert, Isidre|||0000-0003-1442-2258
Lacoma, Alicia|||0000-0002-2049-3872
Yero, Daniel|||0000-0002-6234-4082
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bacterial pathogenesis
Biofilms
Pathogens
Clinical microbiology
topic Bacterial pathogenesis
Biofilms
Pathogens
Clinical microbiology
description Dual species interactions in co-isolated pairs of Staphylococcus aureus and Pseudomonas aeruginosa from patients with tracheobronchitis or bronchial colonization were examined. The genetic and phenotypic diversity between the isolates was high making the interactions detected strain-specific. Despite this, and the clinical origin of the strains, some interactions were common between some co-isolated pairs. For most pairs, P. aeruginosa exoproducts affected biofilm formation and reduced growth in vitro in its S. aureus counterpart. Conversely, S. aureus did not impair biofilm formation and stimulated swarming motility in P. aeruginosa. Co-culture in a medium that mimics respiratory mucus promoted coexistence and favored mixed microcolony formation within biofilms. Under these conditions, key genes controlled by quorum sensing were differentially regulated in both species in an isolate-dependent manner. Finally, co-infection in the acute infection model in Galleria mellonella larvae showed an additive effect only in the co-isolated pair in which P. aeruginosa affected less S. aureus growth. This work contributes to understanding the complex interspecies interactions between P. aeruginosa and S. aureus by studying strains isolated during acute infection.
publishDate 2022
dc.date.none.fl_str_mv 2
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/257290
https://dx.doi.org/urn:doi:10.1038/s41598-022-07018-5
url https://ddd.uab.cat/record/257290
https://dx.doi.org/urn:doi:10.1038/s41598-022-07018-5
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI17/01139
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1062
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-111364RB-I00
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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