The release of catecholamines to the cytosol and the exocytosis of secretory vesicles triggered by IP3 in chromaffin cells
The aim of the present study was to investigate the secretory responses elicited by inositol 1,4,5-trisphosphate (IP3) and their regulation by Ca2+ from different sources. Fura-2, carbon fiber amperometry, and plasma membrane capacitance recordings were performed in mouse chromaffin cells to evaluat...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universidad Autónoma de Madrid |
| Repositorio: | Biblos-e Archivo. Repositorio Institucional de la UAM |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.uam.es:10486/731800 |
| Acceso en línea: | https://hdl.handle.net/10486/731800 https://dx.doi.org/10.1152/ajpcell.00328.2025 |
| Access Level: | acceso abierto |
| Palabra clave: | amperometry calcium capacitance catecholamine IP3 Medicina Farmacia |
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The release of catecholamines to the cytosol and the exocytosis of secretory vesicles triggered by IP3 in chromaffin cellsSanz Lázaro, SaraJiménez Pompa, AmandaHernández Vivanco, AliciaCarmona Hidalgo, BeatrizGarcía Magro, NuriaPérez Álvarez, AlbertoCaba González, José CarlosRueda Ruzafa, LolaAlbillos Martínez, María AlmudenaamperometrycalciumcapacitancecatecholamineIP3MedicinaFarmaciaThe aim of the present study was to investigate the secretory responses elicited by inositol 1,4,5-trisphosphate (IP3) and their regulation by Ca2+ from different sources. Fura-2, carbon fiber amperometry, and plasma membrane capacitance recordings were performed in mouse chromaffin cells to evaluate cytosolic Ca2+ changes, catecholamine release, and exocytosis, respectively. Amperometric recordings revealed that IP3 triggered the continuous release of catecholamines to the cytosol with a plateau shape, either applied independently or in combination with the V-ATPase blocker bafilomycin A1, without exhibiting additive effects, which suggests that V-ATPase blockade might be a potential mechanism of action. The catecholamine release elicited by IP3 can take place in the absence of cytosolic Ca2+; however, it may be also regulated by it through a bell-shaped mechanism, with the contribution of Ca2+ stored in intracellular organelles. Furthermore, plasma membrane capacitance recordings showed that IP3 could also elicit exocytosis of secretory vesicles with the participation of intracellular organelle Ca2+ stores. This exocytosis could be regulated by vesicular or cytosolic Ca2+, as shown in experiments with bafilomycin A1 or the Ca2+ chelator BAPTA-AM, respectively, and by kaempferol, an activator of the mitochondrial Ca2+ uniporter, suggesting that mitochondria may exert physiologically this Ca2+ regulatory mechanism. Therefore, in the IP3-mediated secretion, Ca2+ from different sources control the different steps of catecholamine release from the secretory vesicle to the cytosol and then finally to the extracellular spaceThis work was supported by grants from the Spanish Ministry of Science and Innovation to A.A. (grant numbers BFU2008-01382/BFI, BFU2011-27690, and BFU2015-69092)ElsevierDepartamento de FarmacologíaFacultad de MedicinaGobierno de EspañaAgencia Estatal de Investigación20252025-12-08research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10486/731800https://dx.doi.org/10.1152/ajpcell.00328.202541196165reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7318002026-06-23T12:46:27Z |
| dc.title.none.fl_str_mv |
The release of catecholamines to the cytosol and the exocytosis of secretory vesicles triggered by IP3 in chromaffin cells |
| title |
The release of catecholamines to the cytosol and the exocytosis of secretory vesicles triggered by IP3 in chromaffin cells |
| spellingShingle |
The release of catecholamines to the cytosol and the exocytosis of secretory vesicles triggered by IP3 in chromaffin cells Sanz Lázaro, Sara amperometry calcium capacitance catecholamine IP3 Medicina Farmacia |
| title_short |
The release of catecholamines to the cytosol and the exocytosis of secretory vesicles triggered by IP3 in chromaffin cells |
| title_full |
The release of catecholamines to the cytosol and the exocytosis of secretory vesicles triggered by IP3 in chromaffin cells |
| title_fullStr |
The release of catecholamines to the cytosol and the exocytosis of secretory vesicles triggered by IP3 in chromaffin cells |
| title_full_unstemmed |
The release of catecholamines to the cytosol and the exocytosis of secretory vesicles triggered by IP3 in chromaffin cells |
| title_sort |
The release of catecholamines to the cytosol and the exocytosis of secretory vesicles triggered by IP3 in chromaffin cells |
| dc.creator.none.fl_str_mv |
Sanz Lázaro, Sara Jiménez Pompa, Amanda Hernández Vivanco, Alicia Carmona Hidalgo, Beatriz García Magro, Nuria Pérez Álvarez, Alberto Caba González, José Carlos Rueda Ruzafa, Lola Albillos Martínez, María Almudena |
| author |
Sanz Lázaro, Sara |
| author_facet |
Sanz Lázaro, Sara Jiménez Pompa, Amanda Hernández Vivanco, Alicia Carmona Hidalgo, Beatriz García Magro, Nuria Pérez Álvarez, Alberto Caba González, José Carlos Rueda Ruzafa, Lola Albillos Martínez, María Almudena |
| author_role |
author |
| author2 |
Jiménez Pompa, Amanda Hernández Vivanco, Alicia Carmona Hidalgo, Beatriz García Magro, Nuria Pérez Álvarez, Alberto Caba González, José Carlos Rueda Ruzafa, Lola Albillos Martínez, María Almudena |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Departamento de Farmacología Facultad de Medicina Gobierno de España Agencia Estatal de Investigación |
| dc.subject.none.fl_str_mv |
amperometry calcium capacitance catecholamine IP3 Medicina Farmacia |
| topic |
amperometry calcium capacitance catecholamine IP3 Medicina Farmacia |
| description |
The aim of the present study was to investigate the secretory responses elicited by inositol 1,4,5-trisphosphate (IP3) and their regulation by Ca2+ from different sources. Fura-2, carbon fiber amperometry, and plasma membrane capacitance recordings were performed in mouse chromaffin cells to evaluate cytosolic Ca2+ changes, catecholamine release, and exocytosis, respectively. Amperometric recordings revealed that IP3 triggered the continuous release of catecholamines to the cytosol with a plateau shape, either applied independently or in combination with the V-ATPase blocker bafilomycin A1, without exhibiting additive effects, which suggests that V-ATPase blockade might be a potential mechanism of action. The catecholamine release elicited by IP3 can take place in the absence of cytosolic Ca2+; however, it may be also regulated by it through a bell-shaped mechanism, with the contribution of Ca2+ stored in intracellular organelles. Furthermore, plasma membrane capacitance recordings showed that IP3 could also elicit exocytosis of secretory vesicles with the participation of intracellular organelle Ca2+ stores. This exocytosis could be regulated by vesicular or cytosolic Ca2+, as shown in experiments with bafilomycin A1 or the Ca2+ chelator BAPTA-AM, respectively, and by kaempferol, an activator of the mitochondrial Ca2+ uniporter, suggesting that mitochondria may exert physiologically this Ca2+ regulatory mechanism. Therefore, in the IP3-mediated secretion, Ca2+ from different sources control the different steps of catecholamine release from the secretory vesicle to the cytosol and then finally to the extracellular space |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2025 2025-12-08 |
| dc.type.none.fl_str_mv |
research article http://purl.org/coar/resource_type/c_2df8fbb1 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10486/731800 https://dx.doi.org/10.1152/ajpcell.00328.2025 41196165 |
| url |
https://hdl.handle.net/10486/731800 https://dx.doi.org/10.1152/ajpcell.00328.2025 |
| identifier_str_mv |
41196165 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
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reponame:Biblos-e Archivo. Repositorio Institucional de la UAM instname:Universidad Autónoma de Madrid |
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Universidad Autónoma de Madrid |
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Biblos-e Archivo. Repositorio Institucional de la UAM |
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Biblos-e Archivo. Repositorio Institucional de la UAM |
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