Mutual repression between Pax2 and Snail factors regulates the epithelial/mesenchymal state during intermediate mesoderm differentiation
The pronephros is the first renal structure in the embryo, arising by mesenchymal-to-epithelial transition of the intermediate mesoderm, where Pax2 induces epithelialization and differentiation of this mesenchyme. Here, we show in chick embryos that Snail1 is sufficient to keep the intermediate meso...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universidad de Jaén |
| Repositorio: | RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén |
| OAI Identifier: | oai:ruja.ujaen.es:10953/7297 |
| Acceso en línea: | https://hdl.handle.net/10953/7297 |
| Access Level: | acceso abierto |
| Palabra clave: | Intermediate mesoderm, Pax2, Snail1, Bi-stability domain, EMT, MET, Chick 2402 Desarrollo embrionario 2406 Biología molecular 2407 Biología celular |
| Sumario: | The pronephros is the first renal structure in the embryo, arising by mesenchymal-to-epithelial transition of the intermediate mesoderm, where Pax2 induces epithelialization and differentiation of this mesenchyme. Here, we show in chick embryos that Snail1 is sufficient to keep the intermediate mesoderm in an undifferentiated state by directly repressing Pax2 transcription. Reciprocally, Pax2 is sufficient and necessary to induce mesenchymal-to-epithelial transition by directly repressing Snail1 expression. We also show that BMP7 acts as an epithelialization signal by downregulating Snail1 and upregulating Pax2 expression. This, together with the Snail1/Pax2 reciprocal repression, establishes a regulatory loop within the bi-stability domain, a dynamic region of the anteroposterior axis where opposing retinoic acid/fibroblast growth factor gradients converge, and which has been found to regulate differentiation of the neural tube and somites. In conclusion, we show that the antagonism between Snail1 and Pax2 determines the epithelial/mesenchymal state during the differentiation of the intermediate mesoderm and propose that the bi-stability domain extends to the intermediatemesoderm, synchronizing the differentiation of all tissues aligned along the mediolateral embryonic axis. |
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