Unravelling turbot (Scophthalmus maximus) resistance to Aeromonas salmonicida: transcriptomic insights from two full-sibling families with divergent susceptibility

24 pages, 11 figures, 2 tables

Detalles Bibliográficos
Autores: Pereiro, Patricia, Tur, R., García, Miguel, Figueras Huerta, Antonio, Novoa, Beatriz
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/376106
Acceso en línea:http://hdl.handle.net/10261/376106
Access Level:acceso abierto
Palabra clave:Turbot families
Aeromonas salmonicida
Furunculosis
Disease resistance
Transcriptome sequencing
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spelling Unravelling turbot (Scophthalmus maximus) resistance to Aeromonas salmonicida: transcriptomic insights from two full-sibling families with divergent susceptibilityPereiro, PatriciaTur, R.García, MiguelFigueras Huerta, AntonioNovoa, BeatrizTurbot familiesAeromonas salmonicidaFurunculosisDisease resistanceTranscriptome sequencing24 pages, 11 figures, 2 tables[Introduction] Furunculosis, caused by the gram-negative bacterium Aeromonas salmonicida subsp. salmonicida, remains a significant threat to turbot (Scophthalmus maximus) aquaculture. Identifying genetic backgrounds with enhanced disease resistance is critical for improving aquaculture health management, reducing antibiotic dependency, and mitigating economic losses. [Methods] In this study, five full-sibling turbot families were challenged with A. salmonicida, which revealed one family with significantly greater resistance. Transcriptomic analyses (RNA-Seq) were performed on resistant and susceptible families, examining both naïve and 24-h postinfection (hpi) samples from head kidney and liver tissues.[Results] In the absence of infection, differentially expressed genes (DEGs) were identified predominantly in the liver. Following infection, a marked increase in DEGs was observed in the head kidney, with many genes linked to immune functions. Interestingly, the resistant family displayed a more controlled inflammatory response and upregulation of genes related to antigen presentation and T-cell activity in the head kidney at early infection stages, which may have contributed to its increased survival rate. In the liver, transcriptomic differences between the families were associated mainly with cytoskeletal organization, cell cycle regulation, and metabolic processes, including insulin signalling and lipid metabolism, regardless of infection status. Additionally, many DEGs overlapped with previously identified quantitative trait loci (QTLs) associated with resistance to A. salmonicida, providing further insights into the genetic basis of disease resistance. [Discussion] This study represents the first RNA-Seq analysis comparing resistant and susceptible turbot families and contributes valuable knowledge for the development of selective breeding programs targeting disease resistance in turbot and other aquaculture species susceptible to A. salmonicidaThis research was financially supported by projects PID2023-148810OB-C21 from the Spanish Ministerio de Ciencia, Innovación y Universidades (MCIU), the Agencia Estatal de Investigación (AEI) and the European Regional Development Fund (ERDF) (MCIU/AEI/10.13039/501100011033/FEDER, UE), and MetDisFish from the Spanish Ministerio de Agricultura, Pesca y Alimentación (MAPA) and the European Maritime, Fisheries and Aquaculture Fund (EMFAF)Peer reviewedFrontiers MediaMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionMinisterio de Agricultura, Pesca y Alimentación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/376106reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-148810OB-C21The underlying dataset has been published as supplementary material of the article in the publishers platform at DOI https://doi.org/10.3389/fimmu.2024.1522666https://doi.org/10.3389/fimmu.2024.1522666Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3761062026-05-22T06:33:51Z
dc.title.none.fl_str_mv Unravelling turbot (Scophthalmus maximus) resistance to Aeromonas salmonicida: transcriptomic insights from two full-sibling families with divergent susceptibility
title Unravelling turbot (Scophthalmus maximus) resistance to Aeromonas salmonicida: transcriptomic insights from two full-sibling families with divergent susceptibility
spellingShingle Unravelling turbot (Scophthalmus maximus) resistance to Aeromonas salmonicida: transcriptomic insights from two full-sibling families with divergent susceptibility
Pereiro, Patricia
Turbot families
Aeromonas salmonicida
Furunculosis
Disease resistance
Transcriptome sequencing
title_short Unravelling turbot (Scophthalmus maximus) resistance to Aeromonas salmonicida: transcriptomic insights from two full-sibling families with divergent susceptibility
title_full Unravelling turbot (Scophthalmus maximus) resistance to Aeromonas salmonicida: transcriptomic insights from two full-sibling families with divergent susceptibility
title_fullStr Unravelling turbot (Scophthalmus maximus) resistance to Aeromonas salmonicida: transcriptomic insights from two full-sibling families with divergent susceptibility
title_full_unstemmed Unravelling turbot (Scophthalmus maximus) resistance to Aeromonas salmonicida: transcriptomic insights from two full-sibling families with divergent susceptibility
title_sort Unravelling turbot (Scophthalmus maximus) resistance to Aeromonas salmonicida: transcriptomic insights from two full-sibling families with divergent susceptibility
dc.creator.none.fl_str_mv Pereiro, Patricia
Tur, R.
García, Miguel
Figueras Huerta, Antonio
Novoa, Beatriz
author Pereiro, Patricia
author_facet Pereiro, Patricia
Tur, R.
García, Miguel
Figueras Huerta, Antonio
Novoa, Beatriz
author_role author
author2 Tur, R.
García, Miguel
Figueras Huerta, Antonio
Novoa, Beatriz
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Ministerio de Agricultura, Pesca y Alimentación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Turbot families
Aeromonas salmonicida
Furunculosis
Disease resistance
Transcriptome sequencing
topic Turbot families
Aeromonas salmonicida
Furunculosis
Disease resistance
Transcriptome sequencing
description 24 pages, 11 figures, 2 tables
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/376106
url http://hdl.handle.net/10261/376106
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-148810OB-C21
The underlying dataset has been published as supplementary material of the article in the publishers platform at DOI https://doi.org/10.3389/fimmu.2024.1522666
https://doi.org/10.3389/fimmu.2024.1522666
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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