Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232

Objectives: Genomic characterization of the internationally spread sequence type (ST) 16 carbapenemresistant Klebsiella pneumoniae. Methods: The complete genomes of three carbapenem producing ST16 K. pneumoniae from Italian patients were analysed by single-nucleotide polymorphism-based phylogeny, co...

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Autores: Espinal, P, Nucleo, E, Caltagirone, M, Marchetti, VM, Fernandes, MR, Biscaro, V, Rigoli, R, Carattoli, A, Migliavacca, R, Villa, L
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
Repositorio:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
OAI Identifier:oai:iibsantpau.fundanetsuite.com:p2945
Acceso en línea:https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=2945
Access Level:acceso abierto
Palabra clave:Antimicrobial resistance
Carbapenemase
Healthcare-associated infections
IncF
Outbreak
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spelling Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232Espinal, PNucleo, ECaltagirone, MMarchetti, VMFernandes, MRBiscaro, VRigoli, RCarattoli, AMigliavacca, RVilla, LAntimicrobial resistanceCarbapenemaseHealthcare-associated infectionsIncFOutbreakObjectives: Genomic characterization of the internationally spread sequence type (ST) 16 carbapenemresistant Klebsiella pneumoniae. Methods: The complete genomes of three carbapenem producing ST16 K. pneumoniae from Italian patients were analysed by single-nucleotide polymorphism-based phylogeny, core genome multilocus sequence typing, resistance, plasmid, and virulence content and compared with ten genomes of ST16 strains isolated in other countries. Plasmids carrying blaNDM-1 or blaOXA-232 carbapenemase genes were assembled and sequences were analysed. Results: The internationally spread ST16 K. pneumoniae clone showed variability in terms of distribution of NDM-1 and OXA-232 type carbapenemases. In some ST16 strains, up to six plasmids can be simultaneously present in the same cell, including ColE-like plasmids carrying blaOXA-232 and IncF plasmids carrying blaNDM-1. The differences observed in plasmid, resistance, and virulence content and core genome suggested that there is not a unique, highly conserved ST16 clone, but instead different variants of this lineage circulate worldwide. Conclusions: The ST16 K. pneumoniae clone has spread worldwide and may become a high-risk clone. (c) 2018 Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases.ELSEVIER SCI LTD2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=2945CLINICAL MICROBIOLOGY AND INFECTIONISSN: 1198743XISSNe: 14690691reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pauinstname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)Inglésinfo:eu-repo/semantics/openAccessoai:iibsantpau.fundanetsuite.com:p29452026-06-14T12:41:47Z
dc.title.none.fl_str_mv Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232
title Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232
spellingShingle Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232
Espinal, P
Antimicrobial resistance
Carbapenemase
Healthcare-associated infections
IncF
Outbreak
title_short Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232
title_full Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232
title_fullStr Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232
title_full_unstemmed Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232
title_sort Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232
dc.creator.none.fl_str_mv Espinal, P
Nucleo, E
Caltagirone, M
Marchetti, VM
Fernandes, MR
Biscaro, V
Rigoli, R
Carattoli, A
Migliavacca, R
Villa, L
author Espinal, P
author_facet Espinal, P
Nucleo, E
Caltagirone, M
Marchetti, VM
Fernandes, MR
Biscaro, V
Rigoli, R
Carattoli, A
Migliavacca, R
Villa, L
author_role author
author2 Nucleo, E
Caltagirone, M
Marchetti, VM
Fernandes, MR
Biscaro, V
Rigoli, R
Carattoli, A
Migliavacca, R
Villa, L
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Antimicrobial resistance
Carbapenemase
Healthcare-associated infections
IncF
Outbreak
topic Antimicrobial resistance
Carbapenemase
Healthcare-associated infections
IncF
Outbreak
description Objectives: Genomic characterization of the internationally spread sequence type (ST) 16 carbapenemresistant Klebsiella pneumoniae. Methods: The complete genomes of three carbapenem producing ST16 K. pneumoniae from Italian patients were analysed by single-nucleotide polymorphism-based phylogeny, core genome multilocus sequence typing, resistance, plasmid, and virulence content and compared with ten genomes of ST16 strains isolated in other countries. Plasmids carrying blaNDM-1 or blaOXA-232 carbapenemase genes were assembled and sequences were analysed. Results: The internationally spread ST16 K. pneumoniae clone showed variability in terms of distribution of NDM-1 and OXA-232 type carbapenemases. In some ST16 strains, up to six plasmids can be simultaneously present in the same cell, including ColE-like plasmids carrying blaOXA-232 and IncF plasmids carrying blaNDM-1. The differences observed in plasmid, resistance, and virulence content and core genome suggested that there is not a unique, highly conserved ST16 clone, but instead different variants of this lineage circulate worldwide. Conclusions: The ST16 K. pneumoniae clone has spread worldwide and may become a high-risk clone. (c) 2018 Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=2945
url https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=2945
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ELSEVIER SCI LTD
publisher.none.fl_str_mv ELSEVIER SCI LTD
dc.source.none.fl_str_mv CLINICAL MICROBIOLOGY AND INFECTION
ISSN: 1198743X
ISSNe: 14690691
reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
instname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
instname_str Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
reponame_str r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
collection r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
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