Urinary phoretograms performed by capillary electrophoresis in dogs with chronic disease with or without Leishmania infantum infection

Introduction: The study of early markers to detect kidney malfunction has increased in recent years since serum markers, such as creatinine increase when there is a 75% loss of renal mass. Urinary capillary electrophoresis (UCE) is an available laboratory technique that provides an easily interpreta...

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Autores: Navarro Martínez, Paula Fátima, Fernández-Barredo del Amo, Salceda, Gil Vicente, Laura
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Católica de Valencia San Vicente Mártir
Repositorio:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
Idioma:inglés
OAI Identifier:oai:riucv.ucv.es:20.500.12466/4377
Acceso en línea:http://hdl.handle.net/20.500.12466/4377
Access Level:acceso abierto
Palabra clave:Leishmania infantum
Urinary phoretogram
Canine leishmaniasis
Proteinuria
Chronic kidney disease
Renal
Urinary
Capillary electrophoresis
3109 Ciencias Veterinarias
3109.04 Medicina Interna
3205.06 Nefrología
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spelling Urinary phoretograms performed by capillary electrophoresis in dogs with chronic disease with or without Leishmania infantum infectionNavarro Martínez, Paula FátimaFernández-Barredo del Amo, SalcedaGil Vicente, LauraLeishmania infantumUrinary phoretogramCanine leishmaniasisProteinuriaChronic kidney diseaseRenalUrinaryCapillary electrophoresis3109 Ciencias Veterinarias3109.04 Medicina Interna3205.06 NefrologíaIntroduction: The study of early markers to detect kidney malfunction has increased in recent years since serum markers, such as creatinine increase when there is a 75% loss of renal mass. Urinary capillary electrophoresis (UCE) is an available laboratory technique that provides an easily interpretable electrophoretic pattern. This pattern in our study has been divided into five fractions as it is done in serum: fraction 1 migrating in the albumin zone, fraction 2 in the alpha1-globulins zone, fraction 3 in the alpha2-globulins zone, fraction 4 in the beta-globulins zone, and fraction 5 in the gamma globulins zone. UCE can be useful in the early diagnosis of renal disease. Material and methods: In this study, UCE was performed in dogs with azotemia and proteinuria due to chronic kidney disease (CKD) not related to Leishmania infantum (L. infantum) infection (G1, n = 11) and dogs with CKD related to L. infantum infection (G2, n = 17) and compared with reference intervals from healthy dogs (G0, n = 123), with the aim of comparing their phoretograms and assessing changes in the fractions of the phoretograms based on the health status of individuals. Results: Fraction 2 was statistically augmented in dogs with CKD (G1) when compared with the healthy population (G0) and dogs infected by L. infantum (G2). Fraction 3 was statistically increased in dogs with CKD (G1) and dogs infected by L. infantum (G2) compared with G0. Fraction 4 was found to be statistically decreased in dogs with CKD (G1) and dogs infected by L. infantum (G2) compared with G0. Fraction 5 was statistically higher in dogs with L. infantum (G2) compared with G0 and dogs with CKD (G1). No statistical relationship was found between the protein to creatinine ratio and different fractions from the urinary phoretogram in the study population. No statistical relationship was found between serum and urine fractions in the study population. Discussion: The results of the present study suggest that UCE is a promising non-invasive technique that might be used as a part of the diagnostic and follow-up in dogs with kidney disease due to different pathologies.20242024-06-1720222022-11-1820222022-11-18journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12466/4377reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártirinstname:Universidad Católica de Valencia San Vicente MártirInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riucv.ucv.es:20.500.12466/43772026-06-19T08:32:07Z
dc.title.none.fl_str_mv Urinary phoretograms performed by capillary electrophoresis in dogs with chronic disease with or without Leishmania infantum infection
title Urinary phoretograms performed by capillary electrophoresis in dogs with chronic disease with or without Leishmania infantum infection
spellingShingle Urinary phoretograms performed by capillary electrophoresis in dogs with chronic disease with or without Leishmania infantum infection
Navarro Martínez, Paula Fátima
Leishmania infantum
Urinary phoretogram
Canine leishmaniasis
Proteinuria
Chronic kidney disease
Renal
Urinary
Capillary electrophoresis
3109 Ciencias Veterinarias
3109.04 Medicina Interna
3205.06 Nefrología
title_short Urinary phoretograms performed by capillary electrophoresis in dogs with chronic disease with or without Leishmania infantum infection
title_full Urinary phoretograms performed by capillary electrophoresis in dogs with chronic disease with or without Leishmania infantum infection
title_fullStr Urinary phoretograms performed by capillary electrophoresis in dogs with chronic disease with or without Leishmania infantum infection
title_full_unstemmed Urinary phoretograms performed by capillary electrophoresis in dogs with chronic disease with or without Leishmania infantum infection
title_sort Urinary phoretograms performed by capillary electrophoresis in dogs with chronic disease with or without Leishmania infantum infection
dc.creator.none.fl_str_mv Navarro Martínez, Paula Fátima
Fernández-Barredo del Amo, Salceda
Gil Vicente, Laura
author Navarro Martínez, Paula Fátima
author_facet Navarro Martínez, Paula Fátima
Fernández-Barredo del Amo, Salceda
Gil Vicente, Laura
author_role author
author2 Fernández-Barredo del Amo, Salceda
Gil Vicente, Laura
author2_role author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Leishmania infantum
Urinary phoretogram
Canine leishmaniasis
Proteinuria
Chronic kidney disease
Renal
Urinary
Capillary electrophoresis
3109 Ciencias Veterinarias
3109.04 Medicina Interna
3205.06 Nefrología
topic Leishmania infantum
Urinary phoretogram
Canine leishmaniasis
Proteinuria
Chronic kidney disease
Renal
Urinary
Capillary electrophoresis
3109 Ciencias Veterinarias
3109.04 Medicina Interna
3205.06 Nefrología
description Introduction: The study of early markers to detect kidney malfunction has increased in recent years since serum markers, such as creatinine increase when there is a 75% loss of renal mass. Urinary capillary electrophoresis (UCE) is an available laboratory technique that provides an easily interpretable electrophoretic pattern. This pattern in our study has been divided into five fractions as it is done in serum: fraction 1 migrating in the albumin zone, fraction 2 in the alpha1-globulins zone, fraction 3 in the alpha2-globulins zone, fraction 4 in the beta-globulins zone, and fraction 5 in the gamma globulins zone. UCE can be useful in the early diagnosis of renal disease. Material and methods: In this study, UCE was performed in dogs with azotemia and proteinuria due to chronic kidney disease (CKD) not related to Leishmania infantum (L. infantum) infection (G1, n = 11) and dogs with CKD related to L. infantum infection (G2, n = 17) and compared with reference intervals from healthy dogs (G0, n = 123), with the aim of comparing their phoretograms and assessing changes in the fractions of the phoretograms based on the health status of individuals. Results: Fraction 2 was statistically augmented in dogs with CKD (G1) when compared with the healthy population (G0) and dogs infected by L. infantum (G2). Fraction 3 was statistically increased in dogs with CKD (G1) and dogs infected by L. infantum (G2) compared with G0. Fraction 4 was found to be statistically decreased in dogs with CKD (G1) and dogs infected by L. infantum (G2) compared with G0. Fraction 5 was statistically higher in dogs with L. infantum (G2) compared with G0 and dogs with CKD (G1). No statistical relationship was found between the protein to creatinine ratio and different fractions from the urinary phoretogram in the study population. No statistical relationship was found between serum and urine fractions in the study population. Discussion: The results of the present study suggest that UCE is a promising non-invasive technique that might be used as a part of the diagnostic and follow-up in dogs with kidney disease due to different pathologies.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-11-18
2022
2022-11-18
2024
2024-06-17
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12466/4377
url http://hdl.handle.net/20.500.12466/4377
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
instname:Universidad Católica de Valencia San Vicente Mártir
instname_str Universidad Católica de Valencia San Vicente Mártir
reponame_str RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
collection RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
repository.name.fl_str_mv
repository.mail.fl_str_mv
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