Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration.

Biodiesel-derived glycerol was biologically converted to 2,3-butanediol, an organic compound with multiple industrial applications. Recent studies showed that Raoultella terrigena CECT 4519 is an effective biocatalyst for the bioprocess under conventional growing conditions. In the present work, a n...

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Authors: Ripoll Morales, Vanessa, Ladero, Miguel, Santos, Victoria E.
Format: article
Publication Date:2021
Country:España
Institution:Universidad Francisco de Vitoria
Repository:DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
Language:English
OAI Identifier:oai:ddfv.ufv.es:10641/2539
Online Access:http://hdl.handle.net/10641/2539
Access Level:Open access
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spelling Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration.Ripoll Morales, VanessaLadero, MiguelSantos, Victoria E.Biodiesel-derived glycerol was biologically converted to 2,3-butanediol, an organic compound with multiple industrial applications. Recent studies showed that Raoultella terrigena CECT 4519 is an effective biocatalyst for the bioprocess under conventional growing conditions. In the present work, a novel biocatalyst composed by R. terrigena resting cells was evaluated for the first time. Fluid dynamic conditions has been optimized to maximize 2,3-butanediol production in terms of titre, yield, and selectivity. Regarding the effect of initial glycerol concentration in batch runs, no substrate inhibition was detected in the studied conditions (concentrations between 45 and 250 g/L were used). Employing pure glycerol as carbon source, 82.0 g/L 2,3-butanediol titre was achieved, whereas 76.5 g/L were reached using raw glycerol. These numbers involve an achieved yield respect to maximal theoretic yield of 79% and 77%, respectively. A successful kinetic modelling of the bioprocess was developed and it is able to describe both the evolution of concentrations of relevant components with time and the rates calculated at experimental time values. Estimated specific 2,3-butanediol production rate was 0.034 g2,3-BDO/gX⋅h, while the estimated empirical pseudo-stoichiometric coefficient glycerol/2,3-butanediol was 2.52 gGly/g2,3-BDO. The obtained results showed that R. terrigena resting cells is a promising biocatalyst, which provides new opportunities for developing and scaling-up glycerol biorefineries.20212021-01-0120212021-01-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10641/2539reponame:DDFV. Repositorio Institucional de la Universidad Francisco de Vitoriainstname:Universidad Francisco de VitoriaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:ddfv.ufv.es:10641/25392026-06-11T12:44:57Z
dc.title.none.fl_str_mv Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration.
title Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration.
spellingShingle Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration.
Ripoll Morales, Vanessa
title_short Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration.
title_full Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration.
title_fullStr Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration.
title_full_unstemmed Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration.
title_sort Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration.
dc.creator.none.fl_str_mv Ripoll Morales, Vanessa
Ladero, Miguel
Santos, Victoria E.
author Ripoll Morales, Vanessa
author_facet Ripoll Morales, Vanessa
Ladero, Miguel
Santos, Victoria E.
author_role author
author2 Ladero, Miguel
Santos, Victoria E.
author2_role author
author
dc.contributor.none.fl_str_mv
description Biodiesel-derived glycerol was biologically converted to 2,3-butanediol, an organic compound with multiple industrial applications. Recent studies showed that Raoultella terrigena CECT 4519 is an effective biocatalyst for the bioprocess under conventional growing conditions. In the present work, a novel biocatalyst composed by R. terrigena resting cells was evaluated for the first time. Fluid dynamic conditions has been optimized to maximize 2,3-butanediol production in terms of titre, yield, and selectivity. Regarding the effect of initial glycerol concentration in batch runs, no substrate inhibition was detected in the studied conditions (concentrations between 45 and 250 g/L were used). Employing pure glycerol as carbon source, 82.0 g/L 2,3-butanediol titre was achieved, whereas 76.5 g/L were reached using raw glycerol. These numbers involve an achieved yield respect to maximal theoretic yield of 79% and 77%, respectively. A successful kinetic modelling of the bioprocess was developed and it is able to describe both the evolution of concentrations of relevant components with time and the rates calculated at experimental time values. Estimated specific 2,3-butanediol production rate was 0.034 g2,3-BDO/gX⋅h, while the estimated empirical pseudo-stoichiometric coefficient glycerol/2,3-butanediol was 2.52 gGly/g2,3-BDO. The obtained results showed that R. terrigena resting cells is a promising biocatalyst, which provides new opportunities for developing and scaling-up glycerol biorefineries.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10641/2539
url http://hdl.handle.net/10641/2539
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
instname:Universidad Francisco de Vitoria
instname_str Universidad Francisco de Vitoria
reponame_str DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
collection DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
repository.name.fl_str_mv
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