Unraveling assemblage, functions and stability of the gut microbiota of Blattella germanica by antibiotic treatment

Symbiosis between prokaryotes and eukaryotes is a widespread phenomenon that has contributed to the evolution of eukaryotes. In cockroaches, two types of symbionts coexist: an endosymbiont in the fat body (Blattabacterium), and a rich gut microbiota. The transmission mode of Blattabacterium is verti...

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Autores: Domínguez-Santos, Rebeca, Pérez-Cobas, Ana Elena, Artacho, Alejandro, Castro, José A., Talón, Irene, Moya, Andrés, García-Ferris, Carlos, Latorre, Amparo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/220954
Acceso en línea:http://hdl.handle.net/10261/220954
Access Level:acceso abierto
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spelling Unraveling assemblage, functions and stability of the gut microbiota of Blattella germanica by antibiotic treatmentDomínguez-Santos, RebecaPérez-Cobas, Ana ElenaArtacho, AlejandroCastro, José A.Talón, IreneMoya, AndrésGarcía-Ferris, CarlosLatorre, AmparoSymbiosis between prokaryotes and eukaryotes is a widespread phenomenon that has contributed to the evolution of eukaryotes. In cockroaches, two types of symbionts coexist: an endosymbiont in the fat body (Blattabacterium), and a rich gut microbiota. The transmission mode of Blattabacterium is vertical, while the gut microbiota of a new generation is mainly formed by bacterial species present in feces. We have carried out a metagenomic analysis of Blattella germanica populations, treated and non-treated with two antibiotics (vancomycin and ampicillin) over two generations to (1) determine the core of bacterial communities and potential functions of the gut microbiota and (2) to gain insights into the mechanisms of resistance and resilience of the gut microbiota. Our results indicate that the composition and functions of the bacteria were affected by treatment, more severely in the case of vancomycin. Further results demonstrated that in an untreated second-generation population that comes from antibiotic-treated first-generation, the microbiota is not yet stabilized at nymphal stages but can fully recover in adults when feces of a control population were added to the diet. This signifies the existence of a stable core in either composition and functions in lab-reared populations. The high microbiota diversity as well as the observed functional redundancy point toward the microbiota of cockroach hindguts as a robust ecosystem that can recover from perturbations, with recovery being faster when feces are added to the diet.This research was funded by the European Regional Development Fund (ERDF) and Ministerio de Economía y Competitividad (Spain) BFU2015-64322-C2-1-R and Ministerio de Ciencia, Innovación y Universidades (Spain) PGC2018-099344-B-I00 to AL and Conselleria d’Educació, Generalitat Valenciana (Spain), grant number PROMETEO/2018/133 to AM.Peer reviewedFrontiers MediaMinisterio de Economía y Competitividad (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)European CommissionGeneralitat ValencianaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/220954reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-64322-C2-1-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-099344-B-I00PGC2018-099344-B-I00/AEI/10.13039/501100011033https://doi.org/10.3389/fmicb.2020.00487Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2209542026-05-22T06:33:51Z
dc.title.none.fl_str_mv Unraveling assemblage, functions and stability of the gut microbiota of Blattella germanica by antibiotic treatment
title Unraveling assemblage, functions and stability of the gut microbiota of Blattella germanica by antibiotic treatment
spellingShingle Unraveling assemblage, functions and stability of the gut microbiota of Blattella germanica by antibiotic treatment
Domínguez-Santos, Rebeca
title_short Unraveling assemblage, functions and stability of the gut microbiota of Blattella germanica by antibiotic treatment
title_full Unraveling assemblage, functions and stability of the gut microbiota of Blattella germanica by antibiotic treatment
title_fullStr Unraveling assemblage, functions and stability of the gut microbiota of Blattella germanica by antibiotic treatment
title_full_unstemmed Unraveling assemblage, functions and stability of the gut microbiota of Blattella germanica by antibiotic treatment
title_sort Unraveling assemblage, functions and stability of the gut microbiota of Blattella germanica by antibiotic treatment
dc.creator.none.fl_str_mv Domínguez-Santos, Rebeca
Pérez-Cobas, Ana Elena
Artacho, Alejandro
Castro, José A.
Talón, Irene
Moya, Andrés
García-Ferris, Carlos
Latorre, Amparo
author Domínguez-Santos, Rebeca
author_facet Domínguez-Santos, Rebeca
Pérez-Cobas, Ana Elena
Artacho, Alejandro
Castro, José A.
Talón, Irene
Moya, Andrés
García-Ferris, Carlos
Latorre, Amparo
author_role author
author2 Pérez-Cobas, Ana Elena
Artacho, Alejandro
Castro, José A.
Talón, Irene
Moya, Andrés
García-Ferris, Carlos
Latorre, Amparo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Generalitat Valenciana
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Symbiosis between prokaryotes and eukaryotes is a widespread phenomenon that has contributed to the evolution of eukaryotes. In cockroaches, two types of symbionts coexist: an endosymbiont in the fat body (Blattabacterium), and a rich gut microbiota. The transmission mode of Blattabacterium is vertical, while the gut microbiota of a new generation is mainly formed by bacterial species present in feces. We have carried out a metagenomic analysis of Blattella germanica populations, treated and non-treated with two antibiotics (vancomycin and ampicillin) over two generations to (1) determine the core of bacterial communities and potential functions of the gut microbiota and (2) to gain insights into the mechanisms of resistance and resilience of the gut microbiota. Our results indicate that the composition and functions of the bacteria were affected by treatment, more severely in the case of vancomycin. Further results demonstrated that in an untreated second-generation population that comes from antibiotic-treated first-generation, the microbiota is not yet stabilized at nymphal stages but can fully recover in adults when feces of a control population were added to the diet. This signifies the existence of a stable core in either composition and functions in lab-reared populations. The high microbiota diversity as well as the observed functional redundancy point toward the microbiota of cockroach hindguts as a robust ecosystem that can recover from perturbations, with recovery being faster when feces are added to the diet.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/220954
url http://hdl.handle.net/10261/220954
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-64322-C2-1-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-099344-B-I00
PGC2018-099344-B-I00/AEI/10.13039/501100011033
https://doi.org/10.3389/fmicb.2020.00487
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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