Oligomerization enables the selective targeting of an intrinsically disordered region by a small molecule

Intrinsically disordered regions (IDRs) in proteins are increasingly recognized as attractive targets for therapeutic intervention. A number of small molecules interacting with IDRs have been identified, but the lack of persistent secondary and tertiary structure of these regions has led to the prev...

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Detalles Bibliográficos
Autores: Bielskutė, Stasė, Mateos López, Borja, Awawdy, Muhammad, Garcia Cabau, Carla, Niskanen, Henri, Sánchez Zarzalejo, Carolina, Bracaglia, Lorenzo, Pierattelli, Roberta, Felli, Isabella C., Frigolé Vivas, Marta, García Arroyo, Jesús, Riera Escale, Antoni, Hnisz, Denes, Salvatella Giralt, Xavier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/228103
Acceso en línea:https://hdl.handle.net/2445/228103
Access Level:acceso abierto
Palabra clave:Associació molecular
Oligonucleòtids
Conformació de proteïnes
Molecular association
Oligonucleotides
Proteins conformation
Descripción
Sumario:Intrinsically disordered regions (IDRs) in proteins are increasingly recognized as attractive targets for therapeutic intervention. A number of small molecules interacting with IDRs have been identified, but the lack of persistent secondary and tertiary structure of these regions has led to the prevailing view that they cannot be targeted selectively. Here, we show that a small molecule targeting an IDR evaluated in a clinical trial interacts selectively with an oligomeric form of its target, which is more structured than the monomer and is stabilized by interactions involving aromatic residues in partially α- helical regions. The interaction reshapes the conformational ensemble of the target, alters the biophysical properties of its phase- separated condensates in vitro, and attenuates RNA polymerase II recruitment in cells. Our findings provide mechanistic insights into how small molecules can selectively recognize IDRs.