The Contribution of the Polyamine Spermine to Plant Defense
[eng] Polyamines are essential in plant defense, with putrescine (Put), spermidine (Spd), and spermine (Spm) being the most abundant types. In response to various pathogens, including Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), polyamine levels increase, highlighting their importance in imm...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/215985 |
| Acceso en línea: | https://hdl.handle.net/2445/215985 http://hdl.handle.net/10803/692380 |
| Access Level: | acceso abierto |
| Palabra clave: | Poliamines Immunologia Polyamines Immunology |
| Sumario: | [eng] Polyamines are essential in plant defense, with putrescine (Put), spermidine (Spd), and spermine (Spm) being the most abundant types. In response to various pathogens, including Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), polyamine levels increase, highlighting their importance in immune responses. Our research compared the effects of Put and Spm on pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) responses in Arabidopsis. While Put enhances the production of reactive oxygen species (ROS) triggered by PAMPs like flg22, Spm exhibits an inhibitory effect on this ROS burst depending on RBOHD (RESPIRATORY BURST OXIDASE HOMOLOG D). It also attenuates cytosolic calcium influx stimulated by flg22, suggesting a broader influence on PTI signaling pathways. Genome-Wide Association Studies (GWAS) conducted using 136 Arabidopsis accessions from diverse populations aimed to unravel the genetic determinants behind the Spm inhibitory effect on flg22-induced ROS production. This approach identified specific single nucleotide polymorphisms (SNPs), shedding light on candidate genes involved in the process. Additionally,we also found that Pst DC3000 stimulates Put biosynthesis through coronatine perception and jasmonic acid (JA) signaling, independent of salicylic acid (SA). Conversely, Spm deficiency resulted in heightened JA signaling and compromised SA-mediated defense responses, stimulating disease resistance to Botrytis cinerea. Moreover, Spm deficiency was linked to increased endoplasmic reticulum (ER) stress signaling in response to Pst DC3000, suggesting a role for Spm in buffering ER stress during defense. In summary, this research provides valuable insights into the differential contributions of Spm to PTI and broader plant defense mechanisms. |
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