Unexpected allergenic 11S globulin (Act d 12) and 2S albumin (Act d 13) from kiwi seeds as novel masked relevant components in kiwifruit allergy

Background: Kiwifruit is a common cause of IgE-mediated food allergy. Component-resolved diagnosis of kiwifruit allergy suggests that relevant allergenic components are still undiscovered. The presence of allergens in kiwi seeds has not been investigated in detail so far. Objective: To increase the...

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Detalhes bibliográficos
Autores: Sirvent, Sofia, Cantó, Belén, Cuesta-Herranz, Javier, Gómez, Francisca, Canto, Gabriela, Blanca, Natalia, Rodríguez, Rosalía, Villalba, Mayte, Palomares Gracia, Óscar
Formato: artículo
Fecha de publicación:2014
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/34859
Acesso em linha:https://hdl.handle.net/20.500.14352/34859
Access Level:acceso abierto
Palavra-chave:577.1
616-056.3
food allergy
kiwifruit allergy
kiwi seeds
allergen
11S globulin
2S albumin
Act d 12
Act d 13
component-resolved diagnosis
cross-reactivity
Alergología
Bioquímica (Medicina)
3207.01 Alergias
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oai_identifier_str oai:docta.ucm.es:20.500.14352/34859
network_acronym_str ES
network_name_str España
repository_id_str
spelling Unexpected allergenic 11S globulin (Act d 12) and 2S albumin (Act d 13) from kiwi seeds as novel masked relevant components in kiwifruit allergySirvent, SofiaCantó, BelénCuesta-Herranz, JavierGómez, FranciscaCanto, GabrielaBlanca, NataliaRodríguez, RosalíaVillalba, MaytePalomares Gracia, Óscar577.1616-056.3food allergykiwifruit allergykiwi seedsallergen11S globulin2S albuminAct d 12Act d 13component-resolved diagnosiscross-reactivityAlergologíaBioquímica (Medicina)3207.01 AlergiasBackground: Kiwifruit is a common cause of IgE-mediated food allergy. Component-resolved diagnosis of kiwifruit allergy suggests that relevant allergenic components are still undiscovered. The presence of allergens in kiwi seeds has not been investigated in detail so far. Objective: To increase the diagnostic sensitivity of kiwifruit allergy by identifying new allergens that might have been masked due to their specific location in seeds. Methods: Fifty-five kiwifruit allergic patients were included in the study. Immunoblotting, proteomic tools and Edman degradation were used to identify and purify IgE-reactive proteins from kiwi seeds. Circular dichroism, mass spectrometry, immunoblotting, enzyme-linked immunosorbent assay, skin prick test and IgE-inhibition experiments were used for structural and immunological characterization of purified allergens. Results: Two novel allergens, an 11S globulin (Act d 12) and a 2S albumin (Act d 13) have been identified and purified from kiwi seeds. Both purified molecules display the typical structural features reported for other members of their corresponding protein families. Act d 12 and Act d 13 retain IgEbinding capacity and are recognized by 70.9 % and 18.2 %, respectively, of the tested kiwifruit allergic patients. Both purified allergens show in vivo allergenicity and display in vitro IgE-cross reactivity with homologous counterparts from peanut and tree nuts. Conclusion: The identifica tion of 11S globulin Act d 12 and 2S albumin Act d 13 as novel relevant allergens from kiwi seeds and their availability as well-defined molecules might contribute to improve the diagnosis of kiwifruit allergy.ElsevierUniversidad Complutense de Madrid20142014-01-0120142014-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/34859reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/348592026-06-02T12:44:21Z
dc.title.none.fl_str_mv Unexpected allergenic 11S globulin (Act d 12) and 2S albumin (Act d 13) from kiwi seeds as novel masked relevant components in kiwifruit allergy
title Unexpected allergenic 11S globulin (Act d 12) and 2S albumin (Act d 13) from kiwi seeds as novel masked relevant components in kiwifruit allergy
spellingShingle Unexpected allergenic 11S globulin (Act d 12) and 2S albumin (Act d 13) from kiwi seeds as novel masked relevant components in kiwifruit allergy
Sirvent, Sofia
577.1
616-056.3
food allergy
kiwifruit allergy
kiwi seeds
allergen
11S globulin
2S albumin
Act d 12
Act d 13
component-resolved diagnosis
cross-reactivity
Alergología
Bioquímica (Medicina)
3207.01 Alergias
title_short Unexpected allergenic 11S globulin (Act d 12) and 2S albumin (Act d 13) from kiwi seeds as novel masked relevant components in kiwifruit allergy
title_full Unexpected allergenic 11S globulin (Act d 12) and 2S albumin (Act d 13) from kiwi seeds as novel masked relevant components in kiwifruit allergy
title_fullStr Unexpected allergenic 11S globulin (Act d 12) and 2S albumin (Act d 13) from kiwi seeds as novel masked relevant components in kiwifruit allergy
title_full_unstemmed Unexpected allergenic 11S globulin (Act d 12) and 2S albumin (Act d 13) from kiwi seeds as novel masked relevant components in kiwifruit allergy
title_sort Unexpected allergenic 11S globulin (Act d 12) and 2S albumin (Act d 13) from kiwi seeds as novel masked relevant components in kiwifruit allergy
dc.creator.none.fl_str_mv Sirvent, Sofia
Cantó, Belén
Cuesta-Herranz, Javier
Gómez, Francisca
Canto, Gabriela
Blanca, Natalia
Rodríguez, Rosalía
Villalba, Mayte
Palomares Gracia, Óscar
author Sirvent, Sofia
author_facet Sirvent, Sofia
Cantó, Belén
Cuesta-Herranz, Javier
Gómez, Francisca
Canto, Gabriela
Blanca, Natalia
Rodríguez, Rosalía
Villalba, Mayte
Palomares Gracia, Óscar
author_role author
author2 Cantó, Belén
Cuesta-Herranz, Javier
Gómez, Francisca
Canto, Gabriela
Blanca, Natalia
Rodríguez, Rosalía
Villalba, Mayte
Palomares Gracia, Óscar
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.1
616-056.3
food allergy
kiwifruit allergy
kiwi seeds
allergen
11S globulin
2S albumin
Act d 12
Act d 13
component-resolved diagnosis
cross-reactivity
Alergología
Bioquímica (Medicina)
3207.01 Alergias
topic 577.1
616-056.3
food allergy
kiwifruit allergy
kiwi seeds
allergen
11S globulin
2S albumin
Act d 12
Act d 13
component-resolved diagnosis
cross-reactivity
Alergología
Bioquímica (Medicina)
3207.01 Alergias
description Background: Kiwifruit is a common cause of IgE-mediated food allergy. Component-resolved diagnosis of kiwifruit allergy suggests that relevant allergenic components are still undiscovered. The presence of allergens in kiwi seeds has not been investigated in detail so far. Objective: To increase the diagnostic sensitivity of kiwifruit allergy by identifying new allergens that might have been masked due to their specific location in seeds. Methods: Fifty-five kiwifruit allergic patients were included in the study. Immunoblotting, proteomic tools and Edman degradation were used to identify and purify IgE-reactive proteins from kiwi seeds. Circular dichroism, mass spectrometry, immunoblotting, enzyme-linked immunosorbent assay, skin prick test and IgE-inhibition experiments were used for structural and immunological characterization of purified allergens. Results: Two novel allergens, an 11S globulin (Act d 12) and a 2S albumin (Act d 13) have been identified and purified from kiwi seeds. Both purified molecules display the typical structural features reported for other members of their corresponding protein families. Act d 12 and Act d 13 retain IgEbinding capacity and are recognized by 70.9 % and 18.2 %, respectively, of the tested kiwifruit allergic patients. Both purified allergens show in vivo allergenicity and display in vitro IgE-cross reactivity with homologous counterparts from peanut and tree nuts. Conclusion: The identifica tion of 11S globulin Act d 12 and 2S albumin Act d 13 as novel relevant allergens from kiwi seeds and their availability as well-defined molecules might contribute to improve the diagnosis of kiwifruit allergy.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01
2014
2014-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/34859
url https://hdl.handle.net/20.500.14352/34859
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869409331961659392
score 15.301629

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