Exofucosylation of adipose mesenchymal stromal cells alters their secretome profile

Mesenchymal stromal cells (MSCs) constitute the cell type more frequently used in many regenerative medicine approaches due to their exclusive immunomodulatory properties, and they have been reported to mediate profound immunomodulatory effects in vivo. Nevertheless, MSCs do not express essential ad...

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Detalles Bibliográficos
Autores: García-Bernal, David, García-Arranz, Mariano, García-Guillén, Ana I., García-Hernández, Ana M., Blanquer, Miguel, García-Olmo, Damián, Sackstein, Robert, Moraleda, José María, Zapata González, Agustín
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/8069
Acceso en línea:https://hdl.handle.net/20.500.14352/8069
Access Level:acceso abierto
Palabra clave:576.3
Mesenchymal stromal cells
HCELL
Adipose tissue
Secretome
Regenerative medicine
Biología celular (Biología)
2407 Biología Celular
id ES_60c2094385b9b4e4ecec06407967f5d6
oai_identifier_str oai:docta.ucm.es:20.500.14352/8069
network_acronym_str ES
network_name_str España
repository_id_str
spelling Exofucosylation of adipose mesenchymal stromal cells alters their secretome profileGarcía-Bernal, DavidGarcía-Arranz, MarianoGarcía-Guillén, Ana I.García-Hernández, Ana M.Blanquer, MiguelGarcía-Olmo, DamiánSackstein, RobertMoraleda, José MaríaZapata González, Agustín576.3Mesenchymal stromal cellsHCELLAdipose tissueSecretomeRegenerative medicineBiología celular (Biología)2407 Biología CelularMesenchymal stromal cells (MSCs) constitute the cell type more frequently used in many regenerative medicine approaches due to their exclusive immunomodulatory properties, and they have been reported to mediate profound immunomodulatory effects in vivo. Nevertheless, MSCs do not express essential adhesion molecules actively involved in cell migration, a phenotypic feature that hampers their ability to home inflamed tissues following intravenous administration. In this study, we investigated whether modification by fucosylation of murine AdMSCs (mAdMSCs) creates Hematopoietic Cell E-/L-selectin Ligand, the E-selectin-binding CD44 glycoform. This cell surface glycan modification of CD44 has previously shown in preclinical studies to favor trafficking of mAdMSCs to inflamed or injured peripheral tissues. We analyzed the impact that exofucosylation could have in other innate phenotypic and functional properties of MSCs. Compared to unmodified counterparts, fucosylated mAdMSCs demonstrated higher in vitro migration, an altered secretome pattern, including increased expression and secretion of anti-inflammatory molecules, and a higher capacity to inhibit mitogenstimulated splenocyte proliferation under standard culture conditions. Together, these findings indicate that exofucosylation could represent a suitable cell engineering strategy, not only to facilitate the in vivo MSC colonization of damaged tissues after systemic administration, but also to convert MSCs in a more potent immunomodulatory/antiinflammatory cell therapy-based product for the treatment of a variety of autoimmune, inflammatory, and degenerative diseases.Frontiers MediaUniversidad Complutense de Madrid20202020-11-2620202020-11-26journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/8069reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/80692026-06-02T12:44:21Z
dc.title.none.fl_str_mv Exofucosylation of adipose mesenchymal stromal cells alters their secretome profile
title Exofucosylation of adipose mesenchymal stromal cells alters their secretome profile
spellingShingle Exofucosylation of adipose mesenchymal stromal cells alters their secretome profile
García-Bernal, David
576.3
Mesenchymal stromal cells
HCELL
Adipose tissue
Secretome
Regenerative medicine
Biología celular (Biología)
2407 Biología Celular
title_short Exofucosylation of adipose mesenchymal stromal cells alters their secretome profile
title_full Exofucosylation of adipose mesenchymal stromal cells alters their secretome profile
title_fullStr Exofucosylation of adipose mesenchymal stromal cells alters their secretome profile
title_full_unstemmed Exofucosylation of adipose mesenchymal stromal cells alters their secretome profile
title_sort Exofucosylation of adipose mesenchymal stromal cells alters their secretome profile
dc.creator.none.fl_str_mv García-Bernal, David
García-Arranz, Mariano
García-Guillén, Ana I.
García-Hernández, Ana M.
Blanquer, Miguel
García-Olmo, Damián
Sackstein, Robert
Moraleda, José María
Zapata González, Agustín
author García-Bernal, David
author_facet García-Bernal, David
García-Arranz, Mariano
García-Guillén, Ana I.
García-Hernández, Ana M.
Blanquer, Miguel
García-Olmo, Damián
Sackstein, Robert
Moraleda, José María
Zapata González, Agustín
author_role author
author2 García-Arranz, Mariano
García-Guillén, Ana I.
García-Hernández, Ana M.
Blanquer, Miguel
García-Olmo, Damián
Sackstein, Robert
Moraleda, José María
Zapata González, Agustín
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 576.3
Mesenchymal stromal cells
HCELL
Adipose tissue
Secretome
Regenerative medicine
Biología celular (Biología)
2407 Biología Celular
topic 576.3
Mesenchymal stromal cells
HCELL
Adipose tissue
Secretome
Regenerative medicine
Biología celular (Biología)
2407 Biología Celular
description Mesenchymal stromal cells (MSCs) constitute the cell type more frequently used in many regenerative medicine approaches due to their exclusive immunomodulatory properties, and they have been reported to mediate profound immunomodulatory effects in vivo. Nevertheless, MSCs do not express essential adhesion molecules actively involved in cell migration, a phenotypic feature that hampers their ability to home inflamed tissues following intravenous administration. In this study, we investigated whether modification by fucosylation of murine AdMSCs (mAdMSCs) creates Hematopoietic Cell E-/L-selectin Ligand, the E-selectin-binding CD44 glycoform. This cell surface glycan modification of CD44 has previously shown in preclinical studies to favor trafficking of mAdMSCs to inflamed or injured peripheral tissues. We analyzed the impact that exofucosylation could have in other innate phenotypic and functional properties of MSCs. Compared to unmodified counterparts, fucosylated mAdMSCs demonstrated higher in vitro migration, an altered secretome pattern, including increased expression and secretion of anti-inflammatory molecules, and a higher capacity to inhibit mitogenstimulated splenocyte proliferation under standard culture conditions. Together, these findings indicate that exofucosylation could represent a suitable cell engineering strategy, not only to facilitate the in vivo MSC colonization of damaged tissues after systemic administration, but also to convert MSCs in a more potent immunomodulatory/antiinflammatory cell therapy-based product for the treatment of a variety of autoimmune, inflammatory, and degenerative diseases.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-11-26
2020
2020-11-26
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/8069
url https://hdl.handle.net/20.500.14352/8069
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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