A highly sensitive microsystem based on nanomechanical biosensors for genomics applications

Microcantilever-based biosensors are a promising tool to detect biomolecular interactions in a direct way with high accuracy. We show the development of a portable biosensor microsystem able to detect nucleic acid hybridization with high sensitivity. The microsystem comprises an array of 20 micromec...

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Autores: Lechuga, Laura M., Tamayo de Miguel, Francisco Javier, Álvarez, Mar, Carrascosa, Laura G., Yúfera García, Alberto, Rueda Rueda, Adoración, Plaza, José Antonio, Zinoviev, Kirill, Domínguez Matas, Carlos, Zaballos, Ángel, Moreno, M., Martínez Alonso, Carlos, Díaz, Vicente, Bernad, Antonio
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2006
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/77477
Acceso en línea:https://hdl.handle.net/11441/77477
https://doi.org/10.1016/j.snb.2006.04.017
Access Level:acceso abierto
Palabra clave:Biosensor
Microsystem
Si technology
Microcantilevers
DNA biochip
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spelling A highly sensitive microsystem based on nanomechanical biosensors for genomics applicationsLechuga, Laura M.Tamayo de Miguel, Francisco JavierÁlvarez, MarCarrascosa, Laura G.Yúfera García, AlbertoRueda Rueda, AdoraciónPlaza, José AntonioZinoviev, KirillDomínguez Matas, CarlosZaballos, ÁngelMoreno, M.Martínez Alonso, CarlosDíaz, VicenteBernad, AntonioBiosensorMicrosystemSi technologyMicrocantileversDNA biochipMicrocantilever-based biosensors are a promising tool to detect biomolecular interactions in a direct way with high accuracy. We show the development of a portable biosensor microsystem able to detect nucleic acid hybridization with high sensitivity. The microsystem comprises an array of 20 micromechanical cantilevers produced in silicon technology, a polymer microfluidic system for delivery of the samples, an array of 20 vertical cavity surface emitting lasers (VCSELs) with collimated beams thanks to an integrated microlens array, an optical coupling element to provide the optical path required, and chips with the photodetectors and the CMOS circuitry for signal acquisition and conditioning, capable of measuring the cantilever deflection with sub-nanometer resolution. Robust immobilization and regeneration procedures have been implemented for the oligonucleotide receptor sequences. In a further innovation, an optical waveguide cantilever transducer has been also developed in order to improve the final performance of the device. This has a number of advantages in terms of a simple optical geometry and improved sensitivity.European Union IST-2001-37239Gobierno de España TIC2002-10473-EElsevierElectrónica y ElectromagnetismoEuropean Union (UE)Gobierno de España2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/77477https://doi.org/10.1016/j.snb.2006.04.017reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésSensors and Actuators B: Chemical, 118 (1-2), 2-10.IST-2001-37239TIC2002-10473-Ehttp://dx.doi.org/10.1016/j.snb.2006.04.017info:eu-repo/semantics/openAccessoai:idus.us.es:11441/774772026-06-17T12:51:07Z
dc.title.none.fl_str_mv A highly sensitive microsystem based on nanomechanical biosensors for genomics applications
title A highly sensitive microsystem based on nanomechanical biosensors for genomics applications
spellingShingle A highly sensitive microsystem based on nanomechanical biosensors for genomics applications
Lechuga, Laura M.
Biosensor
Microsystem
Si technology
Microcantilevers
DNA biochip
title_short A highly sensitive microsystem based on nanomechanical biosensors for genomics applications
title_full A highly sensitive microsystem based on nanomechanical biosensors for genomics applications
title_fullStr A highly sensitive microsystem based on nanomechanical biosensors for genomics applications
title_full_unstemmed A highly sensitive microsystem based on nanomechanical biosensors for genomics applications
title_sort A highly sensitive microsystem based on nanomechanical biosensors for genomics applications
dc.creator.none.fl_str_mv Lechuga, Laura M.
Tamayo de Miguel, Francisco Javier
Álvarez, Mar
Carrascosa, Laura G.
Yúfera García, Alberto
Rueda Rueda, Adoración
Plaza, José Antonio
Zinoviev, Kirill
Domínguez Matas, Carlos
Zaballos, Ángel
Moreno, M.
Martínez Alonso, Carlos
Díaz, Vicente
Bernad, Antonio
author Lechuga, Laura M.
author_facet Lechuga, Laura M.
Tamayo de Miguel, Francisco Javier
Álvarez, Mar
Carrascosa, Laura G.
Yúfera García, Alberto
Rueda Rueda, Adoración
Plaza, José Antonio
Zinoviev, Kirill
Domínguez Matas, Carlos
Zaballos, Ángel
Moreno, M.
Martínez Alonso, Carlos
Díaz, Vicente
Bernad, Antonio
author_role author
author2 Tamayo de Miguel, Francisco Javier
Álvarez, Mar
Carrascosa, Laura G.
Yúfera García, Alberto
Rueda Rueda, Adoración
Plaza, José Antonio
Zinoviev, Kirill
Domínguez Matas, Carlos
Zaballos, Ángel
Moreno, M.
Martínez Alonso, Carlos
Díaz, Vicente
Bernad, Antonio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Electrónica y Electromagnetismo
European Union (UE)
Gobierno de España
dc.subject.none.fl_str_mv Biosensor
Microsystem
Si technology
Microcantilevers
DNA biochip
topic Biosensor
Microsystem
Si technology
Microcantilevers
DNA biochip
description Microcantilever-based biosensors are a promising tool to detect biomolecular interactions in a direct way with high accuracy. We show the development of a portable biosensor microsystem able to detect nucleic acid hybridization with high sensitivity. The microsystem comprises an array of 20 micromechanical cantilevers produced in silicon technology, a polymer microfluidic system for delivery of the samples, an array of 20 vertical cavity surface emitting lasers (VCSELs) with collimated beams thanks to an integrated microlens array, an optical coupling element to provide the optical path required, and chips with the photodetectors and the CMOS circuitry for signal acquisition and conditioning, capable of measuring the cantilever deflection with sub-nanometer resolution. Robust immobilization and regeneration procedures have been implemented for the oligonucleotide receptor sequences. In a further innovation, an optical waveguide cantilever transducer has been also developed in order to improve the final performance of the device. This has a number of advantages in terms of a simple optical geometry and improved sensitivity.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/77477
https://doi.org/10.1016/j.snb.2006.04.017
url https://hdl.handle.net/11441/77477
https://doi.org/10.1016/j.snb.2006.04.017
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sensors and Actuators B: Chemical, 118 (1-2), 2-10.
IST-2001-37239
TIC2002-10473-E
http://dx.doi.org/10.1016/j.snb.2006.04.017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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