Arabidopsis Response Regulator 6 (ARR6) Modulates Plant Cell-Wall Composition and Disease Resistance

[EN] The cytokinin signaling pathway, which is mediated by Arabidopsis response regulator (ARR) proteins, has been involved in the modulation of some disease-resistance responses. Here, we describe novel functions of ARR6 in the control of plant disease-resistance and cell-wall composition. Plants i...

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Autores: Bacete, Laura, Mélida Martínez, Hugo, López, Gemma, Dabos, Patrick, Tremousaygue, Dominique, Denancé, Nicolas, Miedes, Eva, Bulone, Vincent, Goffner, Deborah, Molina, Antonio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/20723
Acceso en línea:https://hdl.handle.net/10612/20723
Access Level:acceso abierto
Palabra clave:Ingeniería agrícola
Arabidopsis Response Regulators (arr)
Plectosphaerella Cucumerina
Ralstonia Solanacearum
Cell Wall
Cytokinin
Damage-associated Molecular Patterns (damps)
Disease Resistance
Immunity
3102 Ingeniería Agrícola
2417.19 Fisiología Vegetal
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oai_identifier_str oai:buleria.unileon.es:10612/20723
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spelling Arabidopsis Response Regulator 6 (ARR6) Modulates Plant Cell-Wall Composition and Disease ResistanceBacete, LauraMélida Martínez, HugoLópez, GemmaDabos, PatrickTremousaygue, DominiqueDenancé, NicolasMiedes, EvaBulone, VincentGoffner, DeborahMolina, AntonioIngeniería agrícolaArabidopsis Response Regulators (arr)Plectosphaerella CucumerinaRalstonia SolanacearumCell WallCytokininDamage-associated Molecular Patterns (damps)Disease ResistanceImmunity3102 Ingeniería Agrícola2417.19 Fisiología Vegetal[EN] The cytokinin signaling pathway, which is mediated by Arabidopsis response regulator (ARR) proteins, has been involved in the modulation of some disease-resistance responses. Here, we describe novel functions of ARR6 in the control of plant disease-resistance and cell-wall composition. Plants impaired in ARR6 function (arr6) were more resistant and susceptible, respectively, to the necrotrophic fungus Plectosphaerella cucumerina and to the vascular bacterium Ralstonia solanacearum, whereas Arabidopsis plants that overexpress ARR6 showed the opposite phenotypes, which further support a role of ARR6 in the modulation of disease-resistance responses against these pathogens. Transcriptomics and metabolomics analyses revealed that, in arr6 plants, canonical disease-resistance pathways, like those activated by defensive phytohormones, were not altered, whereas immune responses triggered by microbe-associated molecular patterns were slightly enhanced. Cell-wall composition of arr6 plants was found to be severely altered compared with that of wild-type plants. Remarkably, pectin-enriched cell-wall fractions extracted from arr6 walls triggered more intense immune responses than those activated by similar wall fractions from wild-type plants, suggesting that arr6 pectin fraction is enriched in wall-related damage-associated molecular patterns, which trigger immune responses. This work supports a novel function of ARR6 in the control of cell-wall composition and disease resistance and reinforces the role of the plant cell wall in the modulation of specific immune responses.SIEuropean Commission grant SignWALLINg-624721Ministerio de Economía y Competitividad grant BIO2012-32910Agence Nationale de la Recherche grant WALLTALK ANR-07-GPLA-014Severo Ochoa Programme for Centres of Excellence in Research&Development grant SEV-2016-0672 (2017-2021)MDPIFisiologia VegetalEscuela de Ingeniería Agraria y Forestal2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://hdl.handle.net/10612/20723reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/MINECO/ Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/ BIO2015-64077-Rinfo:eu-repo/grantAgreement/MINECO/ Programa Estatal de Promoción del Talento y su Empleabilidad/ BES-2013-065010info:eu-repo/grantAgreement/AEI/ Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/ RTI2018-096975-B-I00http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/207232026-06-24T12:43:27Z
dc.title.none.fl_str_mv Arabidopsis Response Regulator 6 (ARR6) Modulates Plant Cell-Wall Composition and Disease Resistance
title Arabidopsis Response Regulator 6 (ARR6) Modulates Plant Cell-Wall Composition and Disease Resistance
spellingShingle Arabidopsis Response Regulator 6 (ARR6) Modulates Plant Cell-Wall Composition and Disease Resistance
Bacete, Laura
Ingeniería agrícola
Arabidopsis Response Regulators (arr)
Plectosphaerella Cucumerina
Ralstonia Solanacearum
Cell Wall
Cytokinin
Damage-associated Molecular Patterns (damps)
Disease Resistance
Immunity
3102 Ingeniería Agrícola
2417.19 Fisiología Vegetal
title_short Arabidopsis Response Regulator 6 (ARR6) Modulates Plant Cell-Wall Composition and Disease Resistance
title_full Arabidopsis Response Regulator 6 (ARR6) Modulates Plant Cell-Wall Composition and Disease Resistance
title_fullStr Arabidopsis Response Regulator 6 (ARR6) Modulates Plant Cell-Wall Composition and Disease Resistance
title_full_unstemmed Arabidopsis Response Regulator 6 (ARR6) Modulates Plant Cell-Wall Composition and Disease Resistance
title_sort Arabidopsis Response Regulator 6 (ARR6) Modulates Plant Cell-Wall Composition and Disease Resistance
dc.creator.none.fl_str_mv Bacete, Laura
Mélida Martínez, Hugo
López, Gemma
Dabos, Patrick
Tremousaygue, Dominique
Denancé, Nicolas
Miedes, Eva
Bulone, Vincent
Goffner, Deborah
Molina, Antonio
author Bacete, Laura
author_facet Bacete, Laura
Mélida Martínez, Hugo
López, Gemma
Dabos, Patrick
Tremousaygue, Dominique
Denancé, Nicolas
Miedes, Eva
Bulone, Vincent
Goffner, Deborah
Molina, Antonio
author_role author
author2 Mélida Martínez, Hugo
López, Gemma
Dabos, Patrick
Tremousaygue, Dominique
Denancé, Nicolas
Miedes, Eva
Bulone, Vincent
Goffner, Deborah
Molina, Antonio
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fisiologia Vegetal
Escuela de Ingeniería Agraria y Forestal
dc.subject.none.fl_str_mv Ingeniería agrícola
Arabidopsis Response Regulators (arr)
Plectosphaerella Cucumerina
Ralstonia Solanacearum
Cell Wall
Cytokinin
Damage-associated Molecular Patterns (damps)
Disease Resistance
Immunity
3102 Ingeniería Agrícola
2417.19 Fisiología Vegetal
topic Ingeniería agrícola
Arabidopsis Response Regulators (arr)
Plectosphaerella Cucumerina
Ralstonia Solanacearum
Cell Wall
Cytokinin
Damage-associated Molecular Patterns (damps)
Disease Resistance
Immunity
3102 Ingeniería Agrícola
2417.19 Fisiología Vegetal
description [EN] The cytokinin signaling pathway, which is mediated by Arabidopsis response regulator (ARR) proteins, has been involved in the modulation of some disease-resistance responses. Here, we describe novel functions of ARR6 in the control of plant disease-resistance and cell-wall composition. Plants impaired in ARR6 function (arr6) were more resistant and susceptible, respectively, to the necrotrophic fungus Plectosphaerella cucumerina and to the vascular bacterium Ralstonia solanacearum, whereas Arabidopsis plants that overexpress ARR6 showed the opposite phenotypes, which further support a role of ARR6 in the modulation of disease-resistance responses against these pathogens. Transcriptomics and metabolomics analyses revealed that, in arr6 plants, canonical disease-resistance pathways, like those activated by defensive phytohormones, were not altered, whereas immune responses triggered by microbe-associated molecular patterns were slightly enhanced. Cell-wall composition of arr6 plants was found to be severely altered compared with that of wild-type plants. Remarkably, pectin-enriched cell-wall fractions extracted from arr6 walls triggered more intense immune responses than those activated by similar wall fractions from wild-type plants, suggesting that arr6 pectin fraction is enriched in wall-related damage-associated molecular patterns, which trigger immune responses. This work supports a novel function of ARR6 in the control of cell-wall composition and disease resistance and reinforces the role of the plant cell wall in the modulation of specific immune responses.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/10612/20723
url https://hdl.handle.net/10612/20723
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/ Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/ BIO2015-64077-R
info:eu-repo/grantAgreement/MINECO/ Programa Estatal de Promoción del Talento y su Empleabilidad/ BES-2013-065010
info:eu-repo/grantAgreement/AEI/ Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/ RTI2018-096975-B-I00
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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