Influence of Malignant Pleural Fluid from Lung Adenocarcinoma Patients on Neutrophil Response

Malignant pleural effusion (MPE) is a common severe complication of advanced lung ad-enocarcinoma (LAC). Neutrophils, an essential component of tumor infiltrates, contribute to tumor progression and their counts in MPE have been associated with worse outcome in LAC. This study aimed to evaluate phen...

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Detalles Bibliográficos
Autores: Mulet Gual, Maria|||0000-0003-3639-3291, Osuna Gómez, Rubén|||0000-0003-2875-4405, Zamora, Carlos|||0000-0003-2962-678X, Porcel, José M.|||0000-0002-2734-8061, Nieto Sáchica, Juan Camilo|||0000-0002-3400-1488, Perea, Lidia|||0000-0002-1624-0012, Pajares Ruiz, Virginia|||0000-0002-0916-527X, Muñoz-Fernandez, Ana M., Calvo, Nuria, Sorolla, Maria Alba, Vidal, Silvia|||0000-0002-3909-6682
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:284180
Acceso en línea:https://ddd.uab.cat/record/284180
https://dx.doi.org/urn:doi:10.3390/cancers14102529
Access Level:acceso abierto
Palabra clave:Malignant pleural fluid
Lung adenocarcinoma
Neutrophils
NETosis
Descripción
Sumario:Malignant pleural effusion (MPE) is a common severe complication of advanced lung ad-enocarcinoma (LAC). Neutrophils, an essential component of tumor infiltrates, contribute to tumor progression and their counts in MPE have been associated with worse outcome in LAC. This study aimed to evaluate phenotypical and functional changes of neutrophils induced by MPE to determine the influence of MPE immunomodulatory factors in neutrophil response and to find a possible association between neutrophil functions and clinical outcomes. Pleural fluid samples were col-lected from 47 LAC and 25 heart failure (HF) patients. We measured neutrophil degranulation products by ELISA, oxidative burst capacity and apoptosis by flow cytometry, and NETosis by fluores-cence. The concentration of degranulation products was higher in MPE-LAC than in PE-HF. Func-tionally, neutrophils cultured with MPE-LAC had enhanced survival and neutrophil extracellular trap (NET) formation but had reduced oxidative burst capacity. In MPE, NETosis was positively associated with MMP-9, P-selectin, and sPD-L1 and clinically related to a worse outcome. This is the first study associating NETs with a worse outcome in MPE. Neutrophils likely contribute to tumor progression through the release of NETs, suggesting that they are a potential therapeutic target in LAC.

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